Host Specificity of Snodgrassella in Eastern and Western Honeybees and Its Effects on Naturally Occurring Deformed Wing Virus Titers DOI Creative Commons

Nihong Zhou,

Shuo Yang, Ruike Wei

и другие.

Insects, Год журнала: 2025, Номер 16(5), С. 478 - 478

Опубликована: Май 1, 2025

Honeybee gut microbiota undergoes long-term adaptive evolution with the host, resulting in host-specific genomic and functional characteristics. However, specific role of host-specialized strains shaping host physiological functions remains understudied. This study investigates specificity core symbiont Snodgrassella A. cerana mellifera, exploring its effects on immune response natural virus suppression through analysis colonization experiments. Genomic revealed that strain from exhibited a larger genome greater gene content compared to cerana. Competitive experiments showed although different origins had similar efficiency displayed clear home-field advantage competitive process. Moreover, Inoculation mellifera native significantly reduced Deformed Wing Virus (DWV) titers, whereas non-native no effect. In contrast, neither altered DWV or Sacbrood (SBV) levels Immune only upregulated defensin 2 while significant changes occurred either strain. These results suggest exhibits at level, influencing both suppression, showing efficacy these roles, especially mellifera.

Язык: Английский

Proteogenomic annotation of T6SS components identified in Bacteroides fragilis secretome DOI Creative Commons
Natalya B. Zakharzhevskaya, Olga Yu. Shagaleeva,

Daria A. Kashatnikova

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 11, 2025

Introduction Bacteroides fragilis (Bf)’s T6SS locus has been characterized and shown to have functional activity in competition experiments. It demonstrated that symbiont nontoxigenic Bf strains a more effective “weapon” effect on pathogenic Bf, which is realized through the of effector-immune (E-I) protein pairs. Intensive study structure led an understanding certain issues related its activity, but exact regulatory mechanisms E-I pair remain unclear. Proteomic annotation components detailed descriptions all immune-effector pairs are currently available. In this research, we performed proteogenomic analysis subsequent proteomic toxigenic BOB25. Material methods Fractionated cells, cultivated media vesicles were prepared for proteome by HPLC-MS/MS. Proteogenomic comparative genomic loci BOB25 carried out comparison with reference genomes following strains: JIM10, NCTC 9343 638R. Results According data obtained, represented types analysed samples. The identified culture cells: ClpV (TssH), TssK, TssC, TssB, Hcp (TssD), TetR. predicted effector AKA51715.1 (VU15_08315) was also detected media. greatest amount proteins, including protein, vesicle samples, observed TEM. Potential effectors, (VU15_08315), AKA51716.1 (VU15_08320), AKA51728.1 (VU15_08385) immune AKA51727.1 (VU15_08380), vesicles. Discussion presence proteins secretome indicates high without bacterial competition. possible used bacteria regulate population size altering different repertoires

Язык: Английский

Процитировано

1
Harnessing the human gut microbiota: an emerging frontier in combatting multidrug-resistant bacteria DOI Creative Commons
Wenwen Ding, Yiwen Cheng, Xia Liu

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 17, 2025

Antimicrobial resistance (AMR) has become a major and escalating global health threat, undermining the effectiveness of current antibiotic antimicrobial therapies. The rise multidrug-resistant bacteria led to increasingly difficult-to-treat infections, resulting in higher morbidity, mortality, healthcare costs. Tackling this crisis requires development novel agents, optimization therapeutic strategies, initiatives infection surveillance control. Recent studies highlight crucial role human gut microbiota defending against AMR pathogens. A balanced protects body through mechanisms such as colonization resistance, positioning it key ally fight AMR. In contrast, dysbiosis disrupts defense, thereby facilitating persistence, colonization, dissemination resistant This review will explore how influence drug-resistant bacterial its involvement various types AMR-related potential for microbiota-targeted therapies, fecal transplantation, prebiotics, probiotics, phage therapy. Elucidating interactions between pathogens provide critical insights developing strategies prevent treat infections. While previous reviews have focused on general impact health, specifically look at latest research evolution spread AMR, highlighting strategies.

Язык: Английский

Процитировано

0
Origin and function of beneficial bacterial symbioses in insects DOI
Martin Kaltenpoth, Laura V. Flórez, Aurélien Vigneron

и другие.

Nature Reviews Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

Язык: Английский

Процитировано

0
Comparative Genomic Profiles of Salmonella Typhimurium and Salmonella Dublin Bovine Isolates from the U.S. Indicate Possible Factors Associated with the Host Adaptation of Salmonella Dublin in the Region DOI Creative Commons
Kingsley E. Bentum,

Emmanuel Kuufire,

Rejoice Nyarku

и другие.

Microorganisms, Год журнала: 2025, Номер 13(4), С. 886 - 886

Опубликована: Апрель 12, 2025

Salmonella Dublin (S. Dublin) and Typhimurium Typhimurium) are commonly linked to bovine salmonellosis. S. is, however, considered a bovine-adapted serovar for primarily infecting thriving in cattle. Using (a generalist serovar) as benchmark, this study investigates genomic factors contributing Dublin’s adaptation cattle hosts the U.S. A total of 1337 787 whole-genome sequences from sources were analyzed with CARD (version 4.0.0), ARG-NOTT 6), AMRfinderPlus 4.0.3) antimicrobial resistance (AMR) genes; VFDB virulence AMRFinderPlus stress Plasmidfinder plasmids. Existing clonal groups among isolates two serovars also investigated using Hierarchical Clustering Core Genome Multi-Locus Sequence Typing (HierCC-cgMLST) model. The results revealed minimal variation isolates. Comparatively, IncX1 plasmid was somewhat exclusively identified each carried an average four plasmids (p-value < 0.05). Furthermore, exhibited higher prevalence AMR genes against key antimicrobials, including aminoglycosides, beta-lactams, tetracyclines, sulfonamides, used production. Additionally, Type VI secretion system tssJKLM hcp2/tssD2, essential colonization, found over 50% these possessing that confer heavy metal stressors, like mercury. These findings suggest is supported by conserved genetic makeup enriched genes, factors, stress-related enabling it colonize persist gut.

Язык: Английский

Процитировано

0
Host Specificity of Snodgrassella in Eastern and Western Honeybees and Its Effects on Naturally Occurring Deformed Wing Virus Titers DOI Creative Commons

Nihong Zhou,

Shuo Yang, Ruike Wei

и другие.

Insects, Год журнала: 2025, Номер 16(5), С. 478 - 478

Опубликована: Май 1, 2025

Honeybee gut microbiota undergoes long-term adaptive evolution with the host, resulting in host-specific genomic and functional characteristics. However, specific role of host-specialized strains shaping host physiological functions remains understudied. This study investigates specificity core symbiont Snodgrassella A. cerana mellifera, exploring its effects on immune response natural virus suppression through analysis colonization experiments. Genomic revealed that strain from exhibited a larger genome greater gene content compared to cerana. Competitive experiments showed although different origins had similar efficiency displayed clear home-field advantage competitive process. Moreover, Inoculation mellifera native significantly reduced Deformed Wing Virus (DWV) titers, whereas non-native no effect. In contrast, neither altered DWV or Sacbrood (SBV) levels Immune only upregulated defensin 2 while significant changes occurred either strain. These results suggest exhibits at level, influencing both suppression, showing efficacy these roles, especially mellifera.

Язык: Английский

Процитировано

0