Review on Biphasic Blood Drying Method for Rapid Pathogen Detection in Bloodstream Infections

SLAS TECHNOLOGY, Год журнала: 2025, Номер unknown, С. 100276 - 100276

Опубликована: Март 1, 2025

Rapid and accurate detection of pathogenic microorganisms in blood is critical for diagnosing life-threatening conditions such as bloodstream infections (BSIs). Current methods the identification bacteria from large volumes (5 mL) involve culture steps followed by DNA extraction/purification/concentration Polymerase Chain Reaction (PCR)-based nucleic acid amplification. extraction amplification directly samples hampered complexity matrix, resulting time-consuming labor-intensive processes. This review delves into recent advancements molecular diagnostics based on drying, coined 'biphasic reaction', highlights this new technique that attempts to overcome limitations traditional sample preparation The biphasic drying method, combination with isothermal loop-mediated (LAMP) or recombinase polymerase (RPA), has recently been shown improve sensitivity bacterial, viral, fungal pathogens ∼1 mL whole blood, while minimizing loss avoiding use kits. Furthermore, approach LAMP be a culture-free method capable detecting clinical CFU/mL ∼2.5 hours. represents significant reduction time compared current procedures bacterial prior PCR paper aims guide identify opportunities future applications technology.

Язык: Английский

Direct Multiplex Detection of Full Length Viral Nucleic Acids Across Multi-Demics

Опубликована: Янв. 1, 2025

Язык: Английский

Mpox diagnosis at POC

Trends in biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский