The Endobiota-estrobolome Study in Reproductive aged Women with Ovarian Endometriosis
Abstract
Background
The
human
body
harbors
greater
than
10
trillion
symbiotic,
microbial
cells
that
contribute
to
our
bodily
functions.
Emerging
evidences
suggest
dysbiosis,
especially
of
the
gut
or
urogenital
system,
may
induce
various
pathological
conditions
altered
estrobolome
and
associate
with
certain
estrogen-dependent
diseases
like
endometriosis.
present
case-control
study
analyzed
enzymatic
expressions,
bacterial
compositions,
variations
estrogen
metabolites
in
fecal,
vaginal,
urinary
samples
patients
without
ovarian
Methods
A
total
38
women
reproductive
age,
24
pathologically-proven
endometriosis
14
(control),
were
analyzed.
Recruited
provided
stool,
urine,
vaginal
before
undergoing
surgeries
for
other
benign
tumors.
Gut
assays
β-glucuronidase
β-glucosidase
conducted
using
an
ELISA
spectrophotometer.
Advanced
liquid
chromatography
mass
spectrometry
samples.
microbiota
detected
16S
ribosomal-RNA
gene
sequencing
further
classified
Institute
Genome
Sciences
bioinformatics
pipeline.
Analyses
species
composition,
diversity,
abundance
compared
between
control
groups.
Statistical
significance
was
determined
t-tests
Wilcoxon
tests
(p
<
0.05).
Results
While
similar
activities,
observed
fecal
both
groups,
group
showed
higher
prevalence
Rothia
genus
whereas
genera
such
as
Megamonas ,
[Eubacterium ]
coprostanoligenes_group ,
Allisonella ,
Ruminiclostridium_5 ,
hallii_group ,
Negativibacillus
significantly
more
abundant
group.
Meanwhile,
besides
statistically
lower
folds
4-methoxyestrone
=
0.046),
2-methoxyestrone
0.043),
2-hydroxyestrone-3-methyl
ether
0.006),
also
revealed
abundance,
richness,
evenness.
Conclusions
current
findings
did
not
demonstrate
obvious
dysbiosis
endometriosis,
families
bacteria
two
groups
could
indicate
metabolism
disturbed
gastrointestinal
microbiota.
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Окт. 17, 2024
Язык: Английский