Reduction of flavonoid content in honeysuckle via Erysiphe lonicerae-mediated inhibition of three essential genes in flavonoid biosynthesis pathways
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Апрель 16, 2024
Honeysuckle,
valued
for
its
wide-ranging
uses
in
medicine,
cuisine,
and
aesthetics,
faces
a
significant
challenge
cultivation
due
to
powdery
mildew,
primarily
caused
by
the
Erysiphe
lonicerae
pathogen.
The
interaction
between
honeysuckle
E.
,
especially
concerning
disease
progression,
remains
insufficiently
understood.
Our
study,
conducted
three
different
locations,
found
that
naturally
infected
with
showed
notable
decreases
total
flavonoid
content,
reductions
of
34.7%,
53.5%,
53.8%
observed
each
respective
site.
Controlled
experiments
supported
these
findings,
indicating
artificial
inoculation
led
20.9%
reduction
levels
over
21
days,
worsening
54.8%
decrease
day
42.
Additionally,
there
was
drop
plant’s
antioxidant
capacity,
reaching
an
81.7%
56
days
after
inoculation.
Metabolomic
analysis
also
revealed
substantial
essential
medicinal
components
such
as
chlorogenic
acid,
luteolin,
quercetin,
isoquercetin,
rutin.
Investigating
gene
expression
marked
relative
LjPAL1
gene,
starting
early
7
post-inoculation
falling
minimal
level
(fold
change
=
0.29)
35.
This
trend
mirrored
consistent
phenylalanine
ammonia-lyase
activity
through
entire
process,
which
decreased
72.3%
56.
Further
sustained
repression
downstream
genes
LjFNHO1
LjFNGT1
closely
linked
.
We
identified
mechanism
inhibits
this
pathway
suggest
may
strategically
weaken
honeysuckle’s
resistance
targeting
key
biosynthetic
pathways,
thereby
facilitating
further
pathogen
invasion.
Based
on
our
we
recommend
two
primary
strategies:
first,
monitoring
constituent
from
-affected
areas
ensure
therapeutic
effectiveness;
second,
emphasizing
prevention
control
measures
against
mildew
persistent
decline
crucial
active
compounds.
Язык: Английский
Population structure and markers analysis associated with powdery mildew resistance in pumpkin, Cucurbita pepo L.
Physiological and Molecular Plant Pathology,
Год журнала:
2024,
Номер
134, С. 102469 - 102469
Опубликована: Ноя. 1, 2024
Язык: Английский
Improvement of crop production in controlled environment agriculture through breeding
Frontiers in Plant Science,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 27, 2025
Controlled
environment
agriculture
(CEA)
represents
one
of
the
fastest-growing
sectors
horticulture.
Production
in
controlled
environments
ranges
from
highly
indoor
with
100%
artificial
lighting
(vertical
farms
or
plant
factories)
to
high-tech
greenhouses
without
supplemental
lighting,
simpler
and
high
tunnels.
Although
food
production
occurs
soil
inside
tunnels,
most
CEA
operations
use
various
hydroponic
systems
meet
crop
irrigation
fertility
needs.
The
expansion
offers
promise
as
a
tool
for
increasing
near
urban
these
do
not
rely
on
arable
agricultural
land.
In
addition,
resilience
climate
instability
by
growing
protective
structures.
Products
harvested
tend
be
quality,
both
internal
external,
are
sought
after
consumers.
Currently,
producers
cultivars
bred
open-field
agriculture.
Because
energy
other
costs
CEA,
only
limited
number
crops
have
proven
themselves
profitable
produce.
One
factor
contributing
this
situation
may
lack
optimized
cultivars.
Indoor
offer
opportunities
breeding
that
ideal
systems.
To
facilitate
specialized
cultivars,
wide
range
tools
available
breeders
help
speed
process
increase
its
efficiency.
This
review
aims
cover
needs
horticultural
either
already
being
produced
potential
production.
It
also
reviews
many
including
genomics-informed
breeding,
marker-assisted
selection,
precision
high-throughput
phenotyping,
sources
germplasm
suitable
breeding.
availability
published
genomes
trait-linked
molecular
markers
should
enable
rapid
progress
CEA-specific
will
drive
growth
industry.
Язык: Английский
Non-Sikkim Cucumber Accessions Resistant to Downy Mildew (Pseudoperonospora cubensis)
Seeds,
Год журнала:
2025,
Номер
4(1), С. 8 - 8
Опубликована: Фев. 6, 2025
Downy
mildew
caused
by
the
oomycete
Pseudoperonospora
cubensis
is
a
devastating
disease
of
cucurbits.
Cucumis
species
are
attacked
pathotype
3
(clade
2)
pathogen,
while
Cucurbita
6
1).
The
Sikkim-type
cucumbers
PI
197088
and
330628
express
high
levels
resistance
against
both
pathotypes
(clades)
pathogen
but
no
green-fruit
cucumber
cultivars
resistant
to
available
on
market.
Here
we
report
several
non-Sikkim
accessions
that
show
downy
in
four
consecutive
seasons.
Mean
%
foliage
with
susceptible
controls
Ilan
SMR-18
was
93%
71%,
respectively,
as
0.2%
1.8%
330628,
respectively.
Twenty-four
green
fruit
were
significantly
more
than
controls.
Five
showed
less
10%
infected
leaf
area
follows:
432870—5%,
390266—7.5%,
418964—8.5%,
390258—8.8%,
G12—10%.
390258
390266
race
1
powdery
2,
whereas
418964
races.
These
may
be
used
breeding
programs
accelerate
production
green-fruit,
disease-resistant
cucumbers.
Язык: Английский
Unlocking the potential of ‘Egusi’ melon (Colocynthis citrullus L.) as a crop for biotechnological improvement
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Март 20, 2025
‘Egusi’
melon
(
Colocynthis
citrullus
L.)
plays
a
critical
role
in
food
security
and
potential
biofuel
production
West
Africa.
Its
seeds
are
valued
for
both
their
nutritional
industrial
applications,
especially
biodiesel
production.
However,
the
crop
faces
significant
challenges,
including
impacts
of
climate
change,
water
scarcity,
declining
arable
land,
increased
pressure
from
pests
diseases.
These
challenges
threaten
stability
may
hinder
its
ability
to
meet
future
demand.
To
address
these
issues,
there
is
growing
need
complement
conventional
breeding
methods
with
biotechnological
approaches.
Molecular
approaches;
genomics,
transcriptomics,
proteomics,
metabolomics;
have
been
utilized
improvement
several
cucurbit
species.
information
on
molecular
very
limited.
The
current
review
focuses
melon,
biology,
uses,
factors
affecting
improvement,
highlights
knowledge
gaps
‘Egusi’.
also
examines
omics
technologies
outlines
importance
genetic
transformation
genome
editing
such
as
CRISPR
that
could
drive
development
more
resilient
high-yielding
‘Egusi’varieties
will
contribute
sustainability
profitability
farming.
Язык: Английский
Transcriptome-oxidoreductases analysis associated with resistance to pumpkin powdery mildew
Scientia Horticulturae,
Год журнала:
2025,
Номер
345, С. 114122 - 114122
Опубликована: Апрель 1, 2025
Язык: Английский
Pathotypes and races of Pseudoperonospora cubensis: Two concepts of virulence differentiation
Plant Pathology,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 4, 2024
Abstract
Pseudoperonospora
cubensis
,
causal
agent
of
cucurbit
downy
mildew,
is
a
common
and
highly
variable
pathogen
vegetables.
Pathogen
virulence
can
be
considered
on
two
different
levels,
that
is,
pathotypes
races.
Pathotypes
characterize
the
interactions
between
isolates
set
genotypes
represented
by
species
and/or
genera
host
plant
family.
Races
are
characterized
(e.g.,
cultivars,
lines,
accessions)
one
(or
very
closely
related
species).
Differential
sets
for
pathotype
race
determination
composed
distinct
(different)
genotypes.
Until
recently,
only
differentiation
was
used
to
P
.
Most
recent
research
variation
in
populations
Czech
Republic
working
with
Cucumis
melo
differential
demonstrated
existence
large
number
races
this
pathogen.
The
capacity
primarily
created
denomination
phenotypes/races
powdery
mildew
(
Golovinomyces
orontii
Podosphaera
xanthii
).
However,
also
efficient
differentiating
phenotypes
This
means
C
independent
plant–pathogen
systems:
mildew.
Results
showed
developing
identical
reaction
patterns
at
level
pathotype,
develop
vice
versa.
It
explained
categories
assessment
variation,
which
should
clearly
defined,
recognized
within
international
scientific
breeding
communities.
Язык: Английский
Research Progress on the Genetic and Molecular Mechanisms of Cucumber (Cucumis sativus L.) Powdery Mildew Resistance
Horticulturae,
Год журнала:
2024,
Номер
10(11), С. 1166 - 1166
Опубликована: Ноя. 3, 2024
As
a
common
fungal
disease,
powdery
mildew
(PM)
is
one
of
the
main
diseases
that
harm
growth
and
development
cucumbers.
Understanding
types
pathogenic
fungus
analysis
genetic
molecular
mechanisms
cucumber
resistance
to
PM
at
level
are
important
when
breeding
disease-resistant
varieties.
The
present
review
summarizes
hazards,
prevention,
control
PM,
it
discusses
inheritance
rules,
markers,
quantitative
trait
locus
(QTL)
mapping,
gene
cloning,
omics,
editing
technology,
providing
research
insights
on
varieties
resistant
PM.
Язык: Английский
Identification of Powdery Mildew Resistance-Related Genes in Butternut Squash (Cucurbita moschata)
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(20), С. 10896 - 10896
Опубликована: Окт. 10, 2024
Powdery
mildew
infection
is
a
significant
challenge
in
butternut
squash
(Cucurbita
moschata)
production
during
winter
Hainan,
China.
The
tropical
climate
of
Hainan
promotes
powdery
infection,
resulting
substantial
yield
losses.
By
utilizing
transcriptome
and
genome
sequencing
data,
SNPs
potential
genes
associated
with
resistance
were
identified.
analysis
differentially
expressed
(DEGs)
following
revealed
several
involved
resistance,
particular
focus
on
(R)
gene
cluster
that
may
be
linked
to
the
observed
resistance.
Two
MLO
clade
V
from
Cucurbita
moschata
not
directly
two
genotypes
studied.
These
findings
are
expected
contribute
development
genetic
tools
for
improving
crops,
thereby
reducing
losses
enhancing
sustainability
other
regions.
Язык: Английский