Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions

Biotechnology Advances, Год журнала: 2019, Номер 37(5), С. 746 - 770

Опубликована: Апрель 8, 2019

Язык: Английский

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Computer-aided engineering of lipases solvent tolerance enhanced their applications in sugar esters synthesis: State of the art

Trends in Food Science & Technology, Год журнала: 2024, Номер 144, С. 104323 - 104323

Опубликована: Янв. 2, 2024

Язык: Английский

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Employment of polysaccharides in enzyme immobilization

Reactive and Functional Polymers, Год журнала: 2021, Номер 167, С. 105005 - 105005

Опубликована: Авг. 3, 2021

Язык: Английский

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Evaluation of Strategies to Produce Highly Porous Cross-Linked Aggregates of Porcine Pancreas Lipase with Magnetic Properties

Molecules, Год журнала: 2018, Номер 23(11), С. 2993 - 2993

Опубликована: Ноя. 16, 2018

The preparation of highly porous magnetic crosslinked aggregates (pm-CLEA) porcine pancreas lipase (PPL) is reported. Some strategies to improve the volumetric activity immobilized biocatalyst were evaluated, such as treatment PPL with enzyme surface-modifying agents (polyethyleneimine or dodecyl aldehyde), co-aggregation protein co-feeders (bovine serum albumin and/or soy protein), use silica nanoparticles functionalized amino groups (SMNPs) separation aid, and starch pore-making agent. combination surface modification aldehyde, SMNPs protein, in presence 0.8% (followed by hydrolysis α-amylase), yielded CLEAs expressing high (immobilization yield around 100% recovered 80%), effectiveness factor (approximately 65% equivalent free activity) stability at 40 °C pH 8.0, i.e., co-aggregated SMNPs/bovine SMNPs/soy retained 80% 50% after 10 h incubation, respectively, while was fully inactivated 2 h. Besides, (pm-SP-CLEAs) showed good performance reusability tributyrin for five 4h-batches.

Язык: Английский

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A review on the production and recovery of sugars from lignocellulosics for use in the synthesis of bioproducts

Industrial Crops and Products, Год журнала: 2022, Номер 186, С. 115213 - 115213

Опубликована: Июнь 27, 2022

Язык: Английский

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Performance of Different Immobilized Lipases in the Syntheses of Short- and Long-Chain Carboxylic Acid Esters by Esterification Reactions in Organic Media

Molecules, Год журнала: 2018, Номер 23(4), С. 766 - 766

Опубликована: Март 27, 2018

Short-chain alkyl esters and sugar are widely used in the food, pharmaceutical cosmetic industries due to their flavor emulsifying characteristics, respectively. Both compounds can be synthesized via biocatalysis using lipases. This work aims compare performance of commercial lipases covalently attached dry acrylic beads functionalized with oxirane groups (lipases from Candida antarctica type B-IMMCALB-T2-350, Pseudomonas fluorescens-IMMAPF-T2-150, Thermomyces lanuginosus-IMMTLL-T2-150) a home-made biocatalyst (lipase fluorescens adsorbed onto silica coated octyl groups, named PFL-octyl-silica) syntheses short- long-chain carboxylic acid esters. Esters properties were synthetized by esterification acetic butyl acids several alcohols (e.g., ethanol, 1-butanol, 1-hexanol, isoamyl alcohol), oleic lauric fructose lactose. All biocatalysts showed similar short-chain esters, conversions ranging 88.9 98.4%. However, PFL-octyl-silica was almost always lower than IMMCALB-T2-350, whose conversion up 96% synthesis oleate. high operational stability organic media, thus having great potential for biotransformations.

Язык: Английский

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Challenges and opportunities for production of C5 sugar fatty acid esters (SFAEs) from renewable resources

Industrial Crops and Products, Год журнала: 2022, Номер 193, С. 116170 - 116170

Опубликована: Дек. 23, 2022

Язык: Английский

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Enzymatic production of xylose esters using degummed soybean oil fatty acids following a hydroesterification strategy

Process Biochemistry, Год журнала: 2024, Номер 142, С. 104 - 112

Опубликована: Апрель 14, 2024

The enzymatic synthesis of xylose fatty acid esters was carried out in two steps: hydrolysis degummed soybean oil (DSO) and esterification the produced purified free acids (FFA) with xylose. Different lipases immobilized on a hydrophobic support were evaluated DSO an experimental design used to optimize reaction. Eversa® Transform 2.0 (EV-Purolite) yielded 93% conversion after 6 h using biocatalyst load 6.53 wt.%, water/oil mass ratio 5.5 temperature 48.4 °C. High operational stability for EV-Purolite found sequential batch strategy possibility concentrating glycerol. In stage, lipase from Thermomyces lanuginosus (TLL-Purolite) B Candida antarctica showed better performance than porcine pancreas EV-Purolite. About 17% FFAs (0.85 molecules FAA per molecule) consumed enzyme 5 wt.% TLL-Purolite FFA/xylose molar 5. Sequential batches low TTL-Purolite due desorption TLL support. final mixture reaction medium containing emulsifying properties similar those commercial surfactant.

Язык: Английский

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Reviewing research on the synthesis of CALB-catalyzed sugar esters incorporating systematic mapping principles

Critical Reviews in Biotechnology, Год журнала: 2021, Номер 41(6), С. 865 - 878

Опубликована: Фев. 28, 2021

Rigorous evidence reviews must follow specific guidelines designed to improve transparency, reproducibility, and minimize biases which traditional are susceptible. While synthesis methods, such as systematic maps, have been used in several research fields, the majority of published realm chemical engineering nonsystematic. In this study, we incorporated principles mapping conduct a literature review covering on sugar fatty acid esters (SFAE) with Candida antarctica lipase B (CALB). Our results showed that simple monosaccharides were most cited sugars among studies conducted. The direct use renewable raw materials frequently available resources produce alternative (SE) was scarcely reported our data set. We found free acids (FFA) commonly acyl donors amongst all publications, lauric, oleic, palmitic accounting for ∼43% occurrences. Tertiary alcohols (ter-butyl alcohol (T-but) 2-methyl-2-butanol (2M2B)) ionic liquids solvents synthesize SE. co-occurence analysis keywords involving solvent terms papers evaluated different reaction media (mostly form bisolvent system), also investigating impact their choice ester productivities. Given potential informing us decisions, article reveals trends spaces across CALB-catalyzed SE research, addition introducing new methodological perspective developing field engineering.

Язык: Английский

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Enzymatic Synthesis of Glucose Fatty Acid Esters Using SCOs as Acyl Group-Donors and Their Biological Activities

Applied Sciences, Год журнала: 2021, Номер 11(6), С. 2700 - 2700

Опубликована: Март 17, 2021

Sugar fatty acid esters, especially glucose esters (GEs), have broad applications in food, cosmetic and pharmaceutical industries. In this research, the moieties derived from polyunsaturated acids containing single-cell oils (SCOs) (i.e., those produced Cunninghamella echinulata, Umbelopsis isabellina Nannochloropsis gaditana, as well olive oil an eicosapentaenoic (EPA) concentrate) were converted into GEs by enzymatic synthesis, using lipases biocatalysts. The GE synthesis was monitored thin-layer chromatography, FTIR situ NMR. It found that carried out immobilized Candida antarctica B lipase very effective, reaching total conversion of reactants. shown EPA-GEs effective against several pathogenic bacteria their activity can be attributed to high EPA content. Furthermore, C. echinulata-GEs more pathogens compared with U. isabellina-GEs, probably due presence gamma linolenic (GLA) lipids which is known for its antimicrobial activity, higher concentrations. also showed strong insecticidal Aedes aegypti larvae, followed EPA-GEs, oil-GEs N. gaditana-GEs. All synthesized induced apoptosis SKOV-3 ovarian cancer cell line, apoptotic rate increasing significantly after 48 h. A percentage observed cells treated echinulata-GEs, isabellina-GEs oil-GEs. We conclude SCOs used interesting biological properties.

Язык: Английский

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