An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium DOI Creative Commons
Yu Jin Kim,

So-Hyeon Baek,

Elena Volynchikova

и другие.

Mycobiology, Год журнала: 2025, Номер 53(1), С. 120 - 127

Опубликована: Янв. 2, 2025

An improved sporulation method for Pythium aphanidermatum on 10% V8-juice agar was developed to generate substantial quantities of zoospores. We conducted experiments using a 4 × factorial design evaluate the effects temperature (20, 25, 30, and 35 °C) incubation time (2, 3, 4, 5 days) zoospore production from isolates HSv05 KACC 48066. The results indicated that 30 °C consistently yielded highest numbers zoospores across all periods. Applying same (30 periods other (CCp03, CCp04, CCp05, HSv10, 40156) also resulted in higher regardless times compared with under control condition (25 2 days). Further virulence tests isolate revealed high concentrations (5 102 - 105 zoospores/ml) induced severe rot symptoms; however, low concentration zoospores/ml produced only considerably weak symptoms inoculated potato tubers. No appeared tubers 0 (uninoculated control) or 101 zoospores/ml. These findings suggest our can help obtain adequate various basic applied studies, including pathogen identification, assessment, resistance resource screening, strategy development.

Язык: Английский

An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium DOI Creative Commons
Yu Jin Kim,

So-Hyeon Baek,

Elena Volynchikova

и другие.

Mycobiology, Год журнала: 2025, Номер 53(1), С. 120 - 127

Опубликована: Янв. 2, 2025

An improved sporulation method for Pythium aphanidermatum on 10% V8-juice agar was developed to generate substantial quantities of zoospores. We conducted experiments using a 4 × factorial design evaluate the effects temperature (20, 25, 30, and 35 °C) incubation time (2, 3, 4, 5 days) zoospore production from isolates HSv05 KACC 48066. The results indicated that 30 °C consistently yielded highest numbers zoospores across all periods. Applying same (30 periods other (CCp03, CCp04, CCp05, HSv10, 40156) also resulted in higher regardless times compared with under control condition (25 2 days). Further virulence tests isolate revealed high concentrations (5 102 - 105 zoospores/ml) induced severe rot symptoms; however, low concentration zoospores/ml produced only considerably weak symptoms inoculated potato tubers. No appeared tubers 0 (uninoculated control) or 101 zoospores/ml. These findings suggest our can help obtain adequate various basic applied studies, including pathogen identification, assessment, resistance resource screening, strategy development.

Язык: Английский

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