Epigallocatechin -3- gallate mitigates diazinon neurotoxicity via suppression of pro-inflammatory genes and upregulation of antioxidant pathways

BMC Neuroscience, Год журнала: 2025, Номер 26(1)

Опубликована: Март 10, 2025

Diazinon is a commonly used organophosphate (OP) insecticide especially in developing countries for the control of insect pests, however, exposure to its toxic impact humans and other non-target species remains an important public health concern. The study aimed investigate effect epigallocatechin -3- gallate (EGCG), abundant green tea plants on neurobehavioural, biochemical, pathological changes brain male Wistar rats following diazinon toxicity. Sixty adult were acclimatized seven days subsequently randomly assigned into six treatment groups as follows: Group I: Control group (0.2 mL distilled water); II: at 3 mg/kg (1% LD50); III: (3 mg/kg) + EGCG (50 mg/kg, ~ 2% IV: (100 5% V: VI: mg/kg). All treatments administered orally once daily 14 days. Neurobehavioural studies, biomarkers oxidative stress, histology, immunohistochemistry, quantitative polymerase chain reaction (RT qPCR) performed. alone impaired recognition memory, increased stress markers altered antioxidant defense brain. It upregulated TNF-α IL-6 genes repressed GPx 4 gene expressions. was also associated with GFAP, Tau, α-SN immunoreactivity. Microscopic examination revealed loss Purkinje hippocampal cells Co-treatment however improved cognition, lowered markers, status suppressed IL-6. In conclusion, findings from this demonstrated that offered protection against diazinon-induced neurotoxicity. Hence, natural sources such fruits vegetables could offer immense benefits by protecting inflammation neurodegenerative disease conditions. Clinical trial number Not applicable.

Язык: Английский

Epigallocatechin -3- gallate mitigates diazinon neurotoxicity via suppression of pro-inflammatory genes and upregulation of antioxidant pathways

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 8, 2024

Diazinon is a commonly used organophosphate (OP) insecticide especially in developing countries for the control of insect pests, however, exposure to its toxic impact humans and other non-target species remains an important public health concern. The study aimed investigate effect epigallocatechin − 3- gallate (EGCG), abundant green tea plants on neurobehavioural, biochemical, pathological changes brain male Wistar rats following diazinon toxicity. Sixty adult were acclimatized seven days subsequently randomly assigned into six treatment groups as follows: Group I: Control group (0.2 mL distilled water); II: at 3 mg/kg (1% LD50) diazinon; III: (3mg/kg) + EGCG (50 mg/kg, ~ 2% LD50); IV: (100 5% V: (50mg/kg) VI: mg/kg). All treatments administered orally once daily 14 days. Neurobehavioural studies, biomarkers oxidative stress, histology, immunohistochemistry, quantitative polymerase chain reaction (RT qPCR) performed. alone impaired recognition memory, increased stress markers altered antioxidant defense brain. It upregulated TNF-α IL-6 genes repressed GPx 4 gene expressions. was also associated with GFAP, Tau, α-SN immunoreactivity. Microscopic examination revealed loss purkinje hippocampal cells Co-treatment however improved cognition, lowered markers, status suppressed IL-6. In conclusion, findings from this demonstrated that offered protection against diazinon-induced neurotoxicity.

Язык: Английский

Neuroprotective effect of Launaea taraxacifolia against neuroinflammation, memory loss and neurobehavioral deficit in a rat model of hypertension: biochemical and immunohistochemical approaches

Journal of Herbmed Pharmacology, Год журнала: 2024, Номер 13(3), С. 390 - 398

Опубликована: Июнь 27, 2024

Introduction: Alterations of antioxidant defense, neuroinflammation, and neurodegeneration are common pathological occurrences associated with neurodegenerative diseases. This study evaluated the neuroprotective effect Launaea taraxacifolia (LT), popularly known as African Wild lettuce, against memory loss, neurobehavioral deficit. Methods: Adult Wistar rats were used following random assignment into groups 1 to 5. Group one was normal control. Groups four five received 40 mg/kg Nω-nitro-l-arginine methyl ester (L-NAME). In addition L-NAME exposure, three 100 200 LT, whereas group 10 lisinopril. The experiment lasted for weeks. Markers oxidative stress, neurobehavioural studies, histology, immunohistochemistry glial fibrillary acidic protein (GFAP), ionised calcium-binding adaptor molecule (Iba-1), well anti-calbindin staining astrocytes, microglia, Purkinje cells determined. Results: Malondialdehyde (MDA) carbonyl in alone heightened compared those treated LT. However, treatment LT significantly reduced neuronal changes. Quantitative analysis immunohistochemical revealed indicated by astrogliosis, microgliosis, cell degeneration untreated rats. Moreover, observed ultrastructural anarchy induced restored (P<0.05). Conclusion: Together, leaf extract can be effective a drug candidate.

Язык: Английский