Characterization of human Aquaporin ion channels in a yeast expression system as a tool for novel ion channel discovery DOI Open Access
Saeed Nourmohammadi, Sam W. Henderson, Sunita A. Ramesh

и другие.

Bioscience Reports, Год журнала: 2024, Номер 44(8)

Опубликована: Июль 29, 2024

Abstract Aquaporin (AQP) channels found in all domains of life are transmembrane proteins which mediate passive transport water, glycerol, signaling molecules, metabolites, and charged solutes. Discovery new classes ion-conducting AQP has been slow, likely reflecting time- labor-intensive methods required for traditional electrophysiology. Work here defines a sensitive mass-throughput system detecting ion channels, identified by rescue cell growth the K+-transport-defective yeast strain CY162 following genetic complementation with heterologously expressed cation-permeable using well characterized human AQP1 channel proof concept. Results showed conferred permeability to cations rescued survival yeast. Comprehensive testing that response properties fully recapitulated pharmacological agonist antagonist profiles activation, inhibition, dose-dependence, structure–function relationships, demonstrating validity screening tool identification drug discovery efforts. This method also provided information on divalent cation blockers AQP1, pH sensitivity antagonists, AQP6. Site-directed mutagenesis regulatory confirmed was mediated introduced channels. Optical monitoring lithium-sensitive photoswitchable probe living cells independently demonstrated monovalent plasma membrane. Ion were consistent those Xenopus laevis oocyte K+-transport defective Escherichia coli. Outcomes establish powerful approach efficient phylogenetically diverse AQPs yet untested functions as

Язык: Английский

Multi- and poly-pharmacology of carbonic anhydrase inhibitors DOI
Claudiu T. Supuran

Pharmacological Reviews, Год журнала: 2024, Номер 77(1), С. 100004 - 100004

Опубликована: Сен. 26, 2024

Eight genetically distinct families of the enzyme carbonic anhydrase (CA, EC 4.2.1.1) have been described in organisms overall phylogenetic tree. They catalyze hydration CO2 to bicarbonate and protons are involved pH regulation, chemosensing, metabolism. The 15 α-CA isoforms present humans pharmacological drug targets known for decades, their inhibitors being used as diuretics, antiglaucoma, antiepileptic, or antiobesity drugs, well management acute mountain sickness, idiopathic intracranial hypertension, recently, antitumor theragnostic agents. Other potential applications include use CA (CAIs) inflammatory conditions, cerebral ischemia, neuropathic pain, Alzheimer/Parkinson disease management. CAs from pathogenic bacteria, fungi, protozoans, nematodes started be considered recent years, with notable advances registered. CAIs a complex multipharmacology probably unique this enzyme, which has exploited intensely but may lead other relevant future due emergence design approaches that afforded highly isoform-selective compounds most α-CAs date. belong multitude chemical classes (sulfonamides isosteres, [iso]coumarins related compounds, mono- dithiocarbamates, selenols, ninhydrines, boronic acids, benzoxaboroles, etc). polypharmacology will also discussed because drugs originally discovered treatment non-CA conditions (topiramate, zonisamide, celecoxib, pazopanib, thiazide, high-ceiling diuretics) show effective inhibition against many CAs, led repurposing diverse applications. SIGNIFICANCE STATEMENT: multiple pharmacologic applications, such antiobesity, antiacute anti-idiopathic drugs. Their neurodegenerations investigated recently. Parasite anhydrases anti-infectives novel mechanisms action can bypass resistance commonly Drugs effectively inhibit these enzymes exert interesting polypharmacologic effects.

Язык: Английский

Процитировано

10

Unveiling the antiurolithiatic potentiality of two benzene sulfonamide derivatives against ethylene glycol-induced renal calculi DOI Creative Commons

Ahmed El-Gendy,

Mohamed S. Nafie,

Zohour I. Nabil

и другие.

Nefrología (English Edition), Год журнала: 2025, Номер 45(2), С. 167 - 181

Опубликована: Фев. 1, 2025

Oxidative stress and inflammation play crucial roles in the onset of kidney injury crystal formation caused by hyperoxaluria. Indapamide is a potent medication for treating renal calculi, but it has severe side effects such as hypokalemia, hypercalcemia, hyperuricemia. Therefore, advisable to explore alternative treatments that do not have these effects. The study aimed reveal antiurolithiatic potential two benzene sulfonamide derivatives (SBCl SBF; A B, respectively) against ethylene glycol-induced stones. rats were divided into main groups: first group consisted 20 with induced stones, second included 15 control rats. This division enabled comparative analysis between stones those without, offering insights stone induction on various physiological parameters biochemical markers. effectiveness (compounds B) was assessed treatment given orally gavage 21 days, administered every 48h after inducing 0.12ml 5% glycol (EG). influence compounds B electrolytes, biochemical, antioxidant, inflammatory reactions kidneys underscores their therapeutic advantages alleviating advancement disease related complications. Both found possess equal inhibiting complications formation. However, SBCl-EG showed superior antioxidant compared SBF-EG. Our study's findings underscore benefits nephrolithiasis oxidative disorders, highlighting responses standard treatments.

Язык: Английский

Процитировано

0

Effectiveness of Topiramate Versus Acetazolamide in the Management of Idiopathic Intracranial Hypertension: ASystematic Review and Meta-Analysis DOI Creative Commons
Abdullah Almaqhawi,

Alia Alokley,

Raghad Saad Alamri

и другие.

Medicina, Год журнала: 2025, Номер 61(3), С. 450 - 450

Опубликована: Март 4, 2025

Background and Objectives: Primary pseudotumor cerebri syndrome, another name for idiopathic intracranial hypertension (IIH), is a neurological condition marked by elevated pressure (ICP) that can result in papilledema without known etiology. The purpose of this study to compare the efficacy topiramate acetazolamide as medical treatments IIH evaluate long-term outcomes both medications. Materials Methods: This systematic review meta-analysis followed PRISMA guidelines was approved International Prospective Register Systematic Reviews (PROSPERO). included randomized clinical trials, retrospective prospective cohort studies, patients with (IIH). Data extraction performed using Rayyan application, risk bias assessed Critical Appraisal Skills Program (CASP). Results: findings revealed statistically significant 67% increase likelihood improvement at 6 months compared baseline administration topiramate. After six drug administration, there 3.6 times decrease visual obscuration baseline. A advantage added benefit weight loss, since obesity modifiable factor. However, remains conventional treatment. Conclusions: found are effective therapies improving metrics decreasing cerebrospinal fluid pressure. Topiramate aids reduction, while recommended its ability lower CSF alleviate changes. combination treatment better results.

Язык: Английский

Процитировано

0

Development of novel amino-benzenesulfonamide derivatives and their analogues as carbonic anhydrase inhibitors: Design, synthesis, anticancer activity assessment, and pharmacokinetic studies using UPLC-MS/MS DOI
Khaled Elsayad, Ghada F. Elmasry, Sally Tarek Mahmoud

и другие.

Bioorganic Chemistry, Год журнала: 2025, Номер 159, С. 108335 - 108335

Опубликована: Март 6, 2025

Язык: Английский

Процитировано

0

Comprehensive Insights into Carbonic Anhydrase Inhibition: A Triad of In vitro, In silico, and In vivo Perspectives DOI
Ahmed A. Allam, Hassan A. Rudayni, Noha Ahmed

и другие.

Enzyme and Microbial Technology, Год журнала: 2025, Номер 189, С. 110657 - 110657

Опубликована: Апрель 17, 2025

Язык: Английский

Процитировано

0

Bacterial ι-CAs DOI
Clemente Capasso, Claudiu T. Supuran

˜The œEnzymes, Год журнала: 2024, Номер unknown, С. 121 - 142

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0

Characterization of human Aquaporin ion channels in a yeast expression system as a tool for novel ion channel discovery DOI Open Access
Saeed Nourmohammadi, Sam W. Henderson, Sunita A. Ramesh

и другие.

Bioscience Reports, Год журнала: 2024, Номер 44(8)

Опубликована: Июль 29, 2024

Abstract Aquaporin (AQP) channels found in all domains of life are transmembrane proteins which mediate passive transport water, glycerol, signaling molecules, metabolites, and charged solutes. Discovery new classes ion-conducting AQP has been slow, likely reflecting time- labor-intensive methods required for traditional electrophysiology. Work here defines a sensitive mass-throughput system detecting ion channels, identified by rescue cell growth the K+-transport-defective yeast strain CY162 following genetic complementation with heterologously expressed cation-permeable using well characterized human AQP1 channel proof concept. Results showed conferred permeability to cations rescued survival yeast. Comprehensive testing that response properties fully recapitulated pharmacological agonist antagonist profiles activation, inhibition, dose-dependence, structure–function relationships, demonstrating validity screening tool identification drug discovery efforts. This method also provided information on divalent cation blockers AQP1, pH sensitivity antagonists, AQP6. Site-directed mutagenesis regulatory confirmed was mediated introduced channels. Optical monitoring lithium-sensitive photoswitchable probe living cells independently demonstrated monovalent plasma membrane. Ion were consistent those Xenopus laevis oocyte K+-transport defective Escherichia coli. Outcomes establish powerful approach efficient phylogenetically diverse AQPs yet untested functions as

Язык: Английский

Процитировано

0