m6A-modified LINC02418 induces transcriptional and post-transcriptional modification of CTNNB1 via interacting with YBX1 and IGF2BP1 in colorectal cancer DOI Creative Commons
Hao Zhang, Ye Han, Chengwei Wu

и другие.

Cell Death Discovery, Год журнала: 2025, Номер 11(1)

Опубликована: Март 13, 2025

Abstract Colorectal cancer (CRC) represents a significant menace to human health, but its molecular pathogenesis remains unclear. Herein, we explored the functional role of LINC02418 in CRC progression. The function was determined through vitro and vivo experiments. mechanism by quantitative real-time PCR (qPCR) analyses, western blot, luciferase reporter assay, methylated RNA immunoprecipitation (MeRIP) pull-down, (RIP) assay chromatin (ChIP) assay. results revealed that expression upregulated tissues high related unfavorable survival patients. Besides, knockdown resulted inhibition proliferation metastasis cells vivo. Mechanistically, found METTL3-mediated m6A modification induced aberrant CRC. could interact with YBX1 enhance DNA-binding ability CTNNB1 promoter, resulting transcriptional activation CTNNB1. In post-transcriptional stage, also stability promoting interaction between IGF2BP1 protein mRNA. What is more, be transcriptionally enhanced protein. Collectively, this study unveils novel oncogenic for might therapeutic target treatment.

Язык: Английский

m6A-modified LINC02418 induces transcriptional and post-transcriptional modification of CTNNB1 via interacting with YBX1 and IGF2BP1 in colorectal cancer DOI Creative Commons
Hao Zhang, Ye Han, Chengwei Wu

и другие.

Cell Death Discovery, Год журнала: 2025, Номер 11(1)

Опубликована: Март 13, 2025

Abstract Colorectal cancer (CRC) represents a significant menace to human health, but its molecular pathogenesis remains unclear. Herein, we explored the functional role of LINC02418 in CRC progression. The function was determined through vitro and vivo experiments. mechanism by quantitative real-time PCR (qPCR) analyses, western blot, luciferase reporter assay, methylated RNA immunoprecipitation (MeRIP) pull-down, (RIP) assay chromatin (ChIP) assay. results revealed that expression upregulated tissues high related unfavorable survival patients. Besides, knockdown resulted inhibition proliferation metastasis cells vivo. Mechanistically, found METTL3-mediated m6A modification induced aberrant CRC. could interact with YBX1 enhance DNA-binding ability CTNNB1 promoter, resulting transcriptional activation CTNNB1. In post-transcriptional stage, also stability promoting interaction between IGF2BP1 protein mRNA. What is more, be transcriptionally enhanced protein. Collectively, this study unveils novel oncogenic for might therapeutic target treatment.

Язык: Английский

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