Dietary probiotic-derived extracellular vesicles as delivery systems of bioactive compounds to maintain intestinal homeostasis

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 159546 - 159546

Опубликована: Янв. 1, 2025

Язык: Английский

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A Simplified Method for the Isolation of Extracellular Vesicles from Probiotic Bacteria and Their Characterization

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 1058 - 1058

Опубликована: Янв. 26, 2025

Probiotic bacteria are normal inhabitants of a healthy human gut, conferring multiple beneficial effects on the gut and beyond. Under various disease states, abundance diversity significantly decreased, process called dysbiosis. Among intra- extracellular components probiotics, vesicles (EVs) secreted by them have recently garnered significant attention as potential mediators probiotics’ host health. Further, these nanosized particles that encapsulate wide range bioactive molecules (proteins, lipids, RNA, DNA) standing out key factors could mediate microbiota–host communication confer ameliorating in experimental inflammatory, metabolic, cardiovascular models. However, standard protocol EV isolation from probiotic bacteria, not varying lab to lab, must be established achieve consistency results pre-clinical Our current study compared two commonly used methods for biological samples, ultracentrifugation precipitation, develop isolating EVs probiotics Lactobacillus acidophilus (LA), Gram-positive bacterium, Escherichia coli Nissle (EcN), Gram-negative bacterium. The method gave ~1.5-fold higher yield both LA EcN precipitation method. released level LA. were quantified characterized nanoparticle-tracking analysis (NTA) measuring specific surface biomarkers using Western blot. Here, we describe our standardized step-by-step their characterization.

Язык: Английский

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Surface-displaying protein from Lacticaseibacillus paracasei–derived extracellular vesicles: Identification and utilization in the fabrication of an endolysin-displaying platform against Staphylococcus aureus

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162196 - 162196

Опубликована: Апрель 1, 2025

Язык: Английский

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The potential use of bacteria and their derivatives as delivery systems for nanoparticles in the treatment of cancer

Journal of drug targeting, Год журнала: 2025, Номер unknown, С. 1 - 54

Опубликована: Апрель 5, 2025

Cancer is a leading cause of mortality and morbidity worldwide. Nanomaterials, unique optical, magnetic, electrical properties at the nanoscale (1-100 nm), have been engineered to improve drug capacity, bioavailability, specificity in cancer treatment. These advancements address toxicity lack selectivity conventional therapies, enabling precise targeting cells, tumor microenvironment, immune system. Among emerging approaches, bacterial treatment shows promise due its natural ability target diverse therapeutic mechanisms, which nanotechnology can further enhance. Bacteria-based delivery systems leverage bacteria's adaptability survival strategies within human body. Bacterial derivatives, such as ghosts (BGs), extracellular vesicles (BEVs), dietary toxins, are recognized effective biological nanomaterials capable carrying nanoparticles (NPs). attracted increasing attention for their potential targeted NP This study explores use various bacteria byproducts vehicles, highlighting treating different types cancer. By combining strengths therapy, these innovative approaches aim revolutionize with improved precision efficacy.

Язык: Английский

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Assessment of Antimicrobial Potential of Lactiplantibacillus plantarum and Their Derived Extracellular Vesicles

Molecular Nutrition & Food Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

ABSTRACT Scope Probiotic‐derived antimicrobial substances offer a promising treatment approach with less resistance risk. This study evaluated the activity of 20 Lactiplantibacillus plantarum strains human gut origin and their derived extracellular vesicles (EVs) against pathogens Staphylococcus aureus MTCC 96 Propionibacterium acnes 1950. Methods results The cell‐free supernatants (CFSs) all Lactobacillus isolates were initially screened for potential selected pathogens. CFSs further concentrated subjected to SDS PAGE, bands excised analysis activity. Additionally, EVs isolated characterized from LpJ5 LpJ15 Results indicated that out isolates, CFS (pH 6.5) both demonstrated highest zones inhibition S. (22.9 ± 1.00 mm) P. (15.66 1.52 mm), respectively. showed 18.5 0.2 mm 0.3 . Conclusion Findings this highlight two potent LpJ15, exhibit substantial activity, suggest application as biotherapeutic agents

Язык: Английский

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Maternal Lactobacillus amylovorus-derived extracellular vesicles modulate M2 macrophage polarization to promote early-life gut development in offspring

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162704 - 162704

Опубликована: Апрель 1, 2025

Язык: Английский

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