The combination of flaxseed lignans and PD-1/ PD-L1 inhibitor inhibits breast cancer growth via modulating gut microbiome and host immunity
Drug Resistance Updates,
Год журнала:
2025,
Номер
80, С. 101222 - 101222
Опубликована: Фев. 28, 2025
Patients
with
breast
cancer
(BC)
who
benefit
from
the
PD-1/PD-L1
inhibitor
(PDi)
is
limited,
necessitating
novel
strategies
to
improve
immunotherapy
efficacy
of
BC.
Here
we
aimed
investigate
inhibitory
effects
flaxseed
lignans
(FL)
on
biological
behaviors
BC
and
evaluate
roles
FL
in
enhancing
anticancer
PDi.
HPLC
was
used
detect
content
enterolactone
(ENL),
bacterial
transformation
product
FL.
Transcript
sequencing
performed
identified
CD38
as
a
downstream
target
gene
ENL.
CD38-overexpressing
cells
were
constructed
cell
proliferation,
colony
formation,
wound
healing
transwell
assays
assess
function
ENL/CD38
axis
vitro.
Multiplexed
immunohistochemistry
(mIHC)
CyTOF
changes
tumor
immune
microenvironment
(TIM).
16S
rDNA
explore
gut
microbiota
mice.
A
series
vivo
experiments
conducted
mechanisms
converted
ENL
by
administration
inhibited
progression
malignant
downregulating
CD38,
key
associated
immunosuppression
blockade
resistance.
The
mIHC
assay
revealed
that
enhanced
CD3+,
CD4+
CD8+
reduced
F4/80+
TIM.
confirmed
regulatory
combination
PDi
(FLcPDi)
In
addition,
analysis
demonstrated
FLcPDi
treatment
significantly
elevated
abundance
Akkermansia
and,
importantly,
response
mice
treated
antibiotics.
FL/ENL/CD38
progression.
modulating
host
immunity.
Язык: Английский
Role of gut microbiome in suppression of cancers
Gut Microbes,
Год журнала:
2025,
Номер
17(1)
Опубликована: Апрель 20, 2025
The
pathogenesis
of
cancer
is
closely
related
to
the
disruption
homeostasis
in
human
body.
gut
microbiome
plays
crucial
roles
maintaining
its
host
throughout
lifespan.
In
recent
years,
a
large
number
studies
have
shown
that
dysbiosis
involved
entire
process
initiation,
development,
and
prognosis
by
influencing
immune
system
metabolism.
Some
specific
intestinal
bacteria
promote
occurrence
development
cancers
under
certain
conditions.
Conversely,
some
other
suppress
oncogenesis
progression
cancers,
including
inhibiting
delaying
boosting
therapeutic
effect
on
cancers.
promoting
effects
been
comprehensively
discussed
previous
review.
This
article
will
review
latest
advances
mechanisms
suppression,
providing
new
perspective
for
developing
strategies
prevention
treatment.
Язык: Английский