Cynarin alleviates intervertebral disc degeneration via protecting nucleus pulposus cells from ferroptosis

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 165, С. 115252 - 115252

Опубликована: Авг. 2, 2023

Intervertebral disc degeneration (IVDD) leads to a series of degenerative spine diseases. Clinical treatment IVDD is mainly surgery, lacking effective drugs alleviate intervertebral degeneration. In this study, we analysed the mRNA sequencing dataset human tissues and revealed participation ferroptosis in IVDD. Furthermore, confirmed that TNF-α, an important cytokine IVDD, induces nucleus pulposus cells. Subsequently, inhibitors screening strategy using multiple indicators was developed. Through screen various natural compounds, cynarin, product enriched Artichoke, discovered inhibit Cynarin can dose-dependently catabolism cells, increase expression key ferroptosis-inhibiting genes (GPX4 NRF2), increment cellular Fe2+, lipid peroxides, reactive oxygen species. It also prevent mitochondria shrinkage, reduce cristae density ferroptosis, rat model. conclusion, cynarin potential candidate for drug development

Язык: Английский

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Drug discovery inspired by bioactive small molecules from nature

Animal Cells and Systems, Год журнала: 2022, Номер 26(6), С. 254 - 265

Опубликована: Ноя. 2, 2022

Natural products (NPs) have greatly contributed to the development of novel treatments for human diseases such as cancer, metabolic disorders, and infections. Compared synthetic chemical compounds, primary secondary metabolites from medicinal plants, fungi, microorganisms, our bodies are promising resources with immense diversity favorable properties drug development. In addition well-validated significance metabolites, endogenous small molecules derived central metabolism signaling events shown great potential candidates due their unique metabolite-protein interactions. this short review, we highlight values NPs, discuss recent scientific technological advances including metabolomics tools, chemoproteomics approaches, artificial intelligence-based computation platforms, explore strategies overcome current challenges in NP-driven discovery.

Язык: Английский

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Research Progress on Anti-Inflammatory Mechanism of Inula cappa

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1911 - 1911

Опубликована: Фев. 23, 2025

The incidence of various inflammatory diseases has remained high. Inula cappa is a kind Chinese herbal medicine with wide range pharmacological uses and application value. It anti-inflammatory, antibacterial, antioxidant, hepatoprotective other activities. monomeric compounds that have been confirmed to anti-inflammatory effects are luteolin, chrysoerilol, artemetin, chlorogenic acid, neochlorogenic cryptchlorogenic isochlorogenic acid A, B, C 1,3-O-dicaffeoylquinic acid. This article introduces the relationship between inflammation, components I. cappa, modulation each component on transduction signal pathway, TLR2/MyD88/NF-KB signaling providing theoretical basis for research clinical medication using cappa.

Язык: Английский

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Artichoke water extract protects against Lead-induced hepatotoxicity by activating Nrf2 signaling and inhibiting NLRP3/caspase-1/GSDMD-mediated pyroptosis

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119654 - 119654

Опубликована: Март 1, 2025

Язык: Английский

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Combined microbiome and metabolomics analysis of yupingfeng san fermented by Bacillus coagulans: insights into probiotic and herbal interactions

PeerJ, Год журнала: 2025, Номер 13, С. e19206 - e19206

Опубликована: Апрель 7, 2025

Background Yupingfeng san is a traditional Chinese medicine formula composed of siler, atractylodes, and astragalus. The herbal fermentation process relies on the role probiotics. Bacillus coagulans probiotic commonly used to ferment food drugs. It produces variety beneficial metabolites during fermentation. However, study interaction between B. yupingfeng still blank. Methods During solid-state san, we metabolomics technology 16S rDNA sequencing analyze differential microbial flora at 0, 3, 7, 11, 15 d, which corresponded groups A0, B3, B7, B11, B15, respectively. This research explored correlation microorganisms in fermented compound medicine. Results results revealed significant difference species β diversity group A0 B ( P < 0.01). At phylum level, Cyanobacteria relative abundance decreased by 6.69%, 9.09%, 5.74%, 2.24%, respectively 0.05). Firmicutes increased 39.73%, 35.65%, 49.09%, 68.66% 0.05), Proteobacteria 39.86% 26.70%, respectively, B11 B15 Actinobacteria initially with extended time, then gradually after reaching its peak B7. genus compared highest level 21.12% B3 9.51% time d. Leuconostoc was significantly higher than (20.93%, 20.73%, 21.00%, Pediococcus also (4.20%, 2.35%, 18.84%, 52.01%, Both Leuconostoc, belong lactic acid bacteria, fivefold, accounting for total 62%. After fermentation, using nontargeted metabolomics, identified 315 metabolites. showed decrease content alkene an increase contents acids, lipids, ketones, amino acids. In addition, quercetin, paeoniflorin-3-O-glucoside, netin, iristin, anthocyanin, caffeic acid, rosmarinic liquiritin, isoliquiritin were upregulated. Conclusion this study, composition metabolic profile studied, it found that rich diversity, Weisseria significantly, while opportunistic pathogens such as Pseudomonas aeruginosa Enterobacter significantly. analysis products lipids ketones increased, small molecular compounds antibacterial effects, these changes worked together inhibit growth maintain intestinal health. not only helps elucidate assembly mechanism functional expression but provides solid scientific basis development efficient safe micro-ecological feed additives.

Язык: Английский

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Exploring the Cardiovascular Potential of Artichoke—A Comprehensive Review

Biology, Год журнала: 2025, Номер 14(4), С. 397 - 397

Опубликована: Апрель 10, 2025

Cardiovascular disease remains a leading cause of morbidity and mortality worldwide, requiring both pharmacological lifestyle-based preventive strategies. Artichoke (Cynara cardunculus L. var. scolymus) has gained attention for its health benefits, including choleretic lipid-lowering activities. However, cardiovascular effects remain underdiscussed. This paper provides critical review the current literature on artichoke, with focus underlying mechanisms action clinical efficacy. Experimental studies assessing artichoke’s endothelial function, vascular smooth muscle relaxation, modulation renin–angiotensin–aldosterone axis were assessed. Additionally, studies, systematic reviews, meta-analyses investigating antihypertensive reviewed. bioactive components, particularly flavonoids caffeoylquinic acids, enhance endothelial-dependent -independent vasorelaxation inhibit angiotensin-converting enzyme activity. Although indicate improvements in flow-mediated dilation, they report only modest reductions blood pressure, high variability formulations, dosages, patient populations. While artichoke supplementation may support pressure regulation health, evidence suggests it should be considered an adjunct rather than replacement conventional therapy. Standardized formulations well-controlled will required to clarify therapeutic role.

Язык: Английский

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Synergistic effects of Lespedeza and artichoke extracts in the therapy of chronic kidney disease: mechanisms and perspectives

KIDNEYS, Год журнала: 2025, Номер 14(1), С. 63 - 70

Опубликована: Март 25, 2025

Chronic kidney disease (CKD) is a major global health issue associated with oxidative stress, inflammation, and fibrosis. Traditional pharmacological approaches have limitations due to side effects insufficient efficacy in the late stages of disease. This study explores potential combining Lespedeza (Lespedeza spp.) artichoke (Cynara scolymus) extracts CKD treatment. The nephroprotective effect extract manifested reduction albuminuria, nitrogen, creatinine levels urine. achieved through both direct antioxidant action, protecting membrane structures, DNA, mitochondria, indirect anti-inflammatory reducing NF-κB inflammasome activity, as well release pro-inflammatory cytokines chemokines. primarily attributed inhibition Wnt/β-catenin signaling pathway, which reduces fibrosis, suppression (IL-1β, IL-2, IL-6, TNF-α, NF-κB, TGF-β1), preventing progression chronic inflammation kidneys. Additionally, inhi­bits angiotensin-converting enzyme, renal hypertension tissue from damage. synergistic may provide multifactorial nephron protection, minimizing slowing fibrosis progression. A li­terature review confirms promise this approach highlights necessity further preclinical clinical stu­dies determine optimal dosing regimens for therapy.

Язык: Английский

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Cynarin, a caffeoylquinic acid derivative in artichoke, inhibits exocytotic glutamate release from rat cortical nerve terminals (synaptosomes)

Neurochemistry International, Год журнала: 2023, Номер 167, С. 105537 - 105537

Опубликована: Май 8, 2023

Язык: Английский

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Fatsia japonica extract exerts antioxidant and anti-neuroinflammatory effects on neuronal cells and a zebrafish model

Journal of Ethnopharmacology, Год журнала: 2024, Номер unknown, С. 117813 - 117813

Опубликована: Янв. 1, 2024

Язык: Английский

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Nrf‐2/ROS/NF‐κB pathway is modulated by cynarin in human mesenchymal stem cells in vitro from ankylosing spondylitis

Clinical and Translational Science, Год журнала: 2024, Номер 17(3)

Опубликована: Март 1, 2024

Ankylosing spondylitis (AS) is an immune chronic inflammatory disease, resulting in back pain, stiffness, and thoracolumbar kyphotic deformity. Based on the reported anti-inflammatory antioxidant capacities of cynarin (Cyn), this study explored its protective role molecular mechanisms mesenchymal stem cells (MSCs) from AS. The target pathways genes were verified using Western blotting, quantitative real-time polymerase chain reaction, immunofluorescent staining, while docking analysis was conducted. In AS-MSCs, we found that expression levels p-NF-κB, IL-6, IL-1β, TNF-α higher IκB-α, Nrf-2, HO-1 lower compared with healthy control (HC)-MSCs. With analysis, biding affinities between Cyn Keap1-Nrf-2 p65-IκB-α predicted. mRNA protein reactive oxygen species (ROS) generation downregulated following administration. Meanwhile, level significantly increased after pretreatment. results suggested AS-MSCs based enhancing antioxidation suppression excessive responses via Nrf-2/ROS/NF-κB axis. Our findings demonstrate a potential candidate for alleviating inflammation

Язык: Английский

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