Antibacterial efficacy of ultrasonically activated probiotic endodontic irrigant against Enterococcus faecalis biofilm: an in-vitro study DOI Creative Commons

Mai Hanafy,

Noha Mohamed Kamal,

Hebatallah Atef Fathallah

и другие.

BMC Oral Health, Год журнала: 2025, Номер 25(1)

Опубликована: Май 26, 2025

Abstract Background Enterococcus faecalis ( E. ) is the most frequently retrieved microorganism from teeth with failed endodontic treatment. Sodium hypochlorite (NaOCl) irrigant still poses some drawbacks, such as its cytotoxic effect and reduced effectiveness when applied at lower concentrations. Root canal disinfection by probiotics may yield positive outcomes due to their proven antibacterial anti-inflammatory abilities. This research was intended assess efficacy of a probiotic after ultrasonic activation against in tooth model. Methods Teeth specimens were infected biofilm then randomly divided into five groups according final flush irrigation protocol used; PRO for irrigant, + activated NaOCl saline irrigation. Activation done 1 min using an Ultra X tip. By counting colony-forming units per milliliter, activity quantitatively evaluated each group pre- post-irrigation application; then, bacterial load reduction percentages calculated accordingly. The one-way ANOVA conducted compare mean values all variables, followed post-hoc Tukey test make comparisons significance level set p < 0.05. Results All experimental exerted CFUs/mL increase percentages. lowest observed group, NaOCl, , PRO, respectively. Statistically significant differences among groups, except which did not exhibit any statistically difference. Conclusion Ultrasonically revealed similar conventional can be effectively used fight biofilm.

Язык: Английский

Antibacterial efficacy of ultrasonically activated probiotic endodontic irrigant against Enterococcus faecalis biofilm: an in-vitro study DOI Creative Commons

Mai Hanafy,

Noha Mohamed Kamal,

Hebatallah Atef Fathallah

и другие.

BMC Oral Health, Год журнала: 2025, Номер 25(1)

Опубликована: Май 26, 2025

Abstract Background Enterococcus faecalis ( E. ) is the most frequently retrieved microorganism from teeth with failed endodontic treatment. Sodium hypochlorite (NaOCl) irrigant still poses some drawbacks, such as its cytotoxic effect and reduced effectiveness when applied at lower concentrations. Root canal disinfection by probiotics may yield positive outcomes due to their proven antibacterial anti-inflammatory abilities. This research was intended assess efficacy of a probiotic after ultrasonic activation against in tooth model. Methods Teeth specimens were infected biofilm then randomly divided into five groups according final flush irrigation protocol used; PRO for irrigant, + activated NaOCl saline irrigation. Activation done 1 min using an Ultra X tip. By counting colony-forming units per milliliter, activity quantitatively evaluated each group pre- post-irrigation application; then, bacterial load reduction percentages calculated accordingly. The one-way ANOVA conducted compare mean values all variables, followed post-hoc Tukey test make comparisons significance level set p < 0.05. Results All experimental exerted CFUs/mL increase percentages. lowest observed group, NaOCl, , PRO, respectively. Statistically significant differences among groups, except which did not exhibit any statistically difference. Conclusion Ultrasonically revealed similar conventional can be effectively used fight biofilm.

Язык: Английский

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