Proteome Analysis of the Nitric Oxide Donor Effect on Pisum Sativum L. Roots DOI
A. M. Egorova,

I. A. Tarchevsky

Applied Biochemistry and Microbiology, Год журнала: 2024, Номер 60(6), С. 1292 - 1300

Опубликована: Дек. 1, 2024

Язык: Английский

Antifungal Activity and Putative Mechanism of HWY-289, a Semisynthetic Protoberberine Derivative, against Botrytis cinerea DOI

Jun‐Xia An,

Bao‐Qi Zhang,

Hongjie Liang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(14), С. 7716 - 7726

Опубликована: Март 27, 2024

The emergence of resistant pathogens has increased the demand for alternative fungicides. use natural products as chemical scaffolds is a potential method developing HWY-289, semisynthetic protoberberine derivative, demonstrated broad-spectrum and potent activities against phytopathogenic fungi, particularly Botrytis cinerea (with EC50 values 1.34 μg/mL). SEM TEM imaging indicated that HWY-289 altered morphology mycelium internal structure cells. Transcriptomics revealed it could break down cellular walls through amino acid sugar nucleotide metabolism. In addition, substantially decreased chitinase activity chitin synthase gene (BcCHSV) expression by 53.03 82.18% at 1.5 μg/mL, respectively. Moreover, this impacted permeability integrity cell membranes. Finally, also hindered energy metabolism, resulting in significant reduction ATP content, ATPase activities, key enzyme TCA cycle. Therefore, may be candidate development plant

Язык: Английский

Процитировано

6
Deciphering the Proteome and Phosphoproteome of Peanut (Arachis hypogaea L.) Pegs Penetrating into the Soil DOI Open Access
Sha Yang,

Mei He,

Zhaohui Tang

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 634 - 634

Опубликована: Янв. 14, 2025

Peanut (Arachis hypogaea L.) is one of the most important crops for oil and protein production. The unique characteristic peanut geocarpy, which means that it blooms aerially gynophores (pegs) penetrate into soil, driving fruit underground. In order to fully understand this phenomenon, we investigated dynamic proteomic phosphoproteomic profiling pegs underground in study. A total 6859 proteins 4142 phosphoproteins with 10,070 phosphosites were identified. data validated quantified using samples randomly selected from arial (APs) (UPs) by parallel reaction monitoring (PRM). Function analyses differentially abundant (DAPs) regulated (DRPPs) exhibited they mainly related stress response, photosynthesis, substance metabolism. Once successfully entered disease-resistant response proteins, such as glutathione S-transferase, peroxidase, cytochrome P450, significantly increased UP adapt new soil environment. abundance photosynthesis-associated provided more photosynthetic products, preparation subsequent pod development. Phosphoproteomics reveals regulatory network synthesis nutrients starch, protein, fatty acid (FA). These results provide insights mechanism, indicating after are inserted phosphorylation involved rapid elongation pegs, accompanied supplying energy development preparing metabolites during following mechanical stimulation darkness.

Язык: Английский

Процитировано

0
The function and regulatory network of sugarcane chitinase gene ScChiIV1 in response to pathogen stress DOI
Yanling Chen, Taotao Huang,

Chuihuai You

и другие.

Plant Physiology and Biochemistry, Год журнала: 2025, Номер unknown, С. 109630 - 109630

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Endophytic chitinase and antifungal metabolites-producing actinobacteria for biological control of cucumber damping off disease DOI

Eman A. El-Akshar,

Rasha M. El-Meihy,

Taha A. Tewfike

и другие.

Journal of Plant Pathology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 14, 2024

Язык: Английский

Процитировано

2
Transcriptome analysis of genes involved in the pathogenesis mechanism of potato virus Y in potato cultivar YouJin DOI Creative Commons
Tianqi Yang,

Xingyue Zhao,

Jinjiang Bai

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Март 6, 2024

Potatoes (

Язык: Английский

Процитировано

1
Development of a rapid and efficient system for CR genes identification based on hairy root transformation in Brassicaceae DOI Creative Commons

Wenlin Yu,

Yang Lu,

Yuanyuan Xiang

и другие.

Horticultural Plant Journal, Год журнала: 2024, Номер 10(4), С. 1049 - 1060

Опубликована: Июль 1, 2024

Many economically important crops and vegetables belonging to the cruciferous family are heavily endangered by clubroot disease caused Plasmodiophora brassicae infection. Breeding of resistant cultivars based on mapping cloning genes is commonly regarded as most cost-effective efficient way fight against this disease. The traditional R gene functional validation requires stable transformation that both time- labor-consuming. In study, a rapid hairy-root transgenic protocol mediated Agrobacterium rhizogenes was developed. positive rate over 80% in B. napus showed GUS reporter only took 1/6 time compared with transformation. system applicable different varieties other including rapa oleracea. particular, two known CR genes, CRA3.7.1 CRA8.2.4 were used respectively, example show works well for study combined subsequent P. infection napus. Most importantly, it over-expression led resistance, RNAi which susceptible phenotype. Therefore, can be batch-wise identification also offered possibility manipulating key within genome could improve our knowledge host-pathogen interaction.

Язык: Английский

Процитировано

1
Chitinase Gene FoChi20 in Fusarium oxysporum Reduces Its Pathogenicity and Improves Disease Resistance in Cotton DOI Open Access

Hui Lou,

Jincheng Zhu,

Zengqiang Zhao

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(15), С. 8517 - 8517

Опубликована: Авг. 4, 2024

Chitinase genes, as a class of cell wall hydrolases, are essential for the development and pathogenesis

Язык: Английский

Процитировано

1
Bioinformatics and functional analysis of EDS1 genes in Brassica napus in response to Plasmodiophora brassicae infection DOI

Jalal Eldeen Chol Atem,

Longcai Gan,

Wenlin Yu

и другие.

Plant Science, Год журнала: 2024, Номер 347, С. 112175 - 112175

Опубликована: Июль 8, 2024

Язык: Английский

Процитировано

0
Carvacrol Effectively Inhibits Pseudomonas tolaasii In Vitro and Induces Resistance to Brown Blotch Disease in Postharvest Agaricus bisporus DOI Creative Commons
Lei Zhang, Rui Song,

Zixuan Shi

и другие.

Foods, Год журнала: 2024, Номер 13(22), С. 3689 - 3689

Опубликована: Ноя. 19, 2024

Carvacrol (CAR), a naturally occurring phenolic monoterpene compound, has recently received attention for its potential use in food preservation. However, whether it is effective controlling brown blotch disease caused by

Язык: Английский

Процитировано

0
Proteome Analysis of the Nitric Oxide Donor Effect on Pisum Sativum L. Roots DOI
A. M. Egorova,

I. A. Tarchevsky

Applied Biochemistry and Microbiology, Год журнала: 2024, Номер 60(6), С. 1292 - 1300

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

0