bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 18, 2024

Abstract Lumpy skin disease (LSD) is a severe infectious, emerging transboundary of cattle, caused by Pox family DNA virus. virus (LSDV) infection associated with febrile response followed emergence widespread dermal nodules. In addition to the skin, LSDV resides in multiple internal organs and can be isolated from blood infected cattle. suggested mechanically transmitted biting arthropods. Live attenuated vaccines are commonly used control its spread. We have characterized tropism, replication, dissemination field isolate an vaccine strain using vitro systems. To follow living cells, we generated recombinant viruses expressing green fluorescent protein (GFP) under synthetic viral promoter. Recombinant, GFP-expressing, LSDVs demonstrated similar replication kinetics their corresponding parental strains bovine kidney cell line (MDBK). further that LSDV-GFP productively replicated macrophage primary foreskin cells no apparent differences between strain. When peripheral mononuclear (PBMCs) were either strain, observed specific driven GFP fluorescence as well significant gene expression. However, PBMCs failed support substantial release infectious progeny. Subsequent analysis anti-viral revealed heat treated (HT) induced expression interferon- stimulated genes (ISGs) PBMCs, but this was suppressed viruses. Finally, show despite recipient co-cultured MDBK produced foci, suggesting potential role dissemination. Highlights Virulent lines fibroblasts. Adherent white susceptible infection. showed active transcription yet genome or production heat-treated not fully upregulated ISGs’ RNA. disseminated contacting permissive cells.

Язык: Английский