Subcellular dynamics in unicellular parasites

Trends in Parasitology, Год журнала: 2025, Номер 41(3), С. 222 - 234

Опубликована: Фев. 10, 2025

Bioimaging has made tremendous advances in this century. Innovations high- and super-resolution microscopy are well established, live-cell imaging is extensively used to gain an overview of dynamic processes. But the combination high spatial temporal resolution necessary capture intracellular dynamics rarely achieved. Further, efficient software pipelines - that can handle recorded data allow comprehensive analyses being developed but lag behind other technical innovations applicability for broad groups researchers. Especially parasites, which offer great potential studying subcellular dynamics, possibilities have only begun be probed. In all cases, complete description molecular movement 3D space remains a challenging necessity.

Язык: Английский

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Intraflagellar transport trains can switch rails and move along multiple microtubules in intact primary cilia

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(16)

Опубликована: Апрель 18, 2025

Structural homeostasis and proper distributions of signaling molecules in cilia require a constant flow cargoes carried by intraflagellar transport (IFT) trains both anterograde retrograde directions within the thin, long ciliary shafts. In motile cilium framework, nine microtubule doublets same length serve as transportation rails, preferential association to two subtubules prevents collisions among IFT that move opposite directions. However, this mechanism is incompatible with primary structure, where most terminate shafts—only several them reach tip only singlet form. Here, we demonstrate interact without apparent preference. They can switch microtubules, they may simultaneously multiple microtubules facilitate their movement. This architecture makes inevitable, live-cell recordings reveal tend pause when come into direct contact. We also find velocity train’s movement often changes after pause. Thus, motion behaviors are distinctive from those cilia, our data offer an essential foundation for understanding molecule cilia.

Язык: Английский

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Discriminating motilities: Coordinating IFT with flagellar beating patterns

The Journal of Cell Biology, Год журнала: 2024, Номер 223(9)

Опубликована: Авг. 7, 2024

Intraflagellar transport has traditionally been studied in immobilized flagella. In this issue, Gray et al. (https://doi.org/10.1083/jcb.202401154) introduced a novel methodology for fast imaging free-swimming Leishmania, revealing the impacts of flagellum immobilization on intraflagellar and its inverse correlation with cell swimming speed.

Язык: Английский

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