A complete collagen IV fluorophore knock-in toolkit reveals α-chain diversity in basement membrane DOI
Sandhya Srinivasan,

Willam Ramos-Lewis,

Mychel Morais

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Abstract The type IV collagen triple helix, composed of three ⍺-chains, is a core basement membrane (BM) component that assembles into network within BMs. Endogenous tagging all ⍺-chains with genetically encoded fluorophores has remained elusive, limiting our understanding this crucial BM component. Through genome editing, we show the C-termini C. elegans EMB-9 and LET-2 can be fused to variety create strain toolkit wild-type health. Using quantitative imaging, results suggest preference for LET-2-LET-2-EMB-9 trimer construction, but also tissue-specific flexibility in trimers assembled driven by differences ⍺-chain expression levels. By emb-9 let-2 mutants model human Gould Syndrome, complex multi-tissue disorder, further discover defects extracellular accumulation turnover might help explain disease pathology. Together, findings identify permissive site will allow diverse studies on regulation function animals. Summary Srinivasan et al., construct fluorophore knock-in using newly identified editing reveal α-chain diversity modeling COL4A1/A2 (Gould) syndrome.

Язык: Английский

A collagen IV fluorophore knock-in toolkit reveals trimer diversity in C. elegans basement membranes DOI
Sandhya Srinivasan, William Ramos-Lewis, Mychel Morais

и другие.

The Journal of Cell Biology, Год журнала: 2025, Номер 224(6)

Опубликована: Март 1, 2025

The type IV collagen triple helix, composed of three ⍺-chains, is a core basement membrane (BM) component that assembles into network within BMs. Endogenous tagging all ⍺-chains with genetically encoded fluorophores has remained elusive, limiting our understanding this crucial BM component. Through genome editing, we show the C termini C. elegans EMB-9 and LET-2 can be fused to variety create strain toolkit wild-type health. Using quantitative imaging, results suggest preference for LET-2-LET-2-EMB-9 trimer construction, but also tissue-specific flexibility in trimers assembled driven by differences ⍺-chain expression levels. By emb-9 let-2 mutants model human Gould syndrome, complex multitissue disorder, further discover defects extracellular accumulation turnover might help explain disease pathology. Together, findings identify permissive site will allow diverse studies on regulation function animals.

Язык: Английский

Процитировано

0
Lipid synthesis leads the way for invasive migration DOI
Laura M. Machesky

The Journal of Cell Biology, Год журнала: 2024, Номер 223(10)

Опубликована: Сен. 10, 2024

Invasive migration requires cells to break through extracellular matrix barriers, which is an energy-expensive process. In this issue, Park et al. (https://doi.org/10.1083/jcb.202402035) highlight the importance of biosynthesis fatty acids, phospholipids, and isoprenoids in driving invasive Caenorhabditis elegans anchor cell a basement membrane barrier during development.

Язык: Английский

Процитировано

0
A complete collagen IV fluorophore knock-in toolkit reveals α-chain diversity in basement membrane DOI
Sandhya Srinivasan,

Willam Ramos-Lewis,

Mychel Morais

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Abstract The type IV collagen triple helix, composed of three ⍺-chains, is a core basement membrane (BM) component that assembles into network within BMs. Endogenous tagging all ⍺-chains with genetically encoded fluorophores has remained elusive, limiting our understanding this crucial BM component. Through genome editing, we show the C-termini C. elegans EMB-9 and LET-2 can be fused to variety create strain toolkit wild-type health. Using quantitative imaging, results suggest preference for LET-2-LET-2-EMB-9 trimer construction, but also tissue-specific flexibility in trimers assembled driven by differences ⍺-chain expression levels. By emb-9 let-2 mutants model human Gould Syndrome, complex multi-tissue disorder, further discover defects extracellular accumulation turnover might help explain disease pathology. Together, findings identify permissive site will allow diverse studies on regulation function animals. Summary Srinivasan et al., construct fluorophore knock-in using newly identified editing reveal α-chain diversity modeling COL4A1/A2 (Gould) syndrome.

Язык: Английский

Процитировано

0