Nanoscale DNA tracing reveals the self-organization mechanism of mitotic chromosomes DOI Creative Commons
Kai Sandvold Beckwith, Andreas Brunner, Natalia Rosalía Morero

и другие.

Cell, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

How genomic DNA is folded during cell division to form the characteristic rod-shaped mitotic chromosomes essential for faithful genome inheritance a long-standing open question in biology. Here, we use nanoscale tracing single dividing cells directly visualize how 3D fold of changes mitosis at scales from loops entire chromosomes. Our structural analysis reveals scaling minimum 6-8 megabases mitosis. Combined with data-driven modeling and molecular perturbations, can show that very large strongly overlapping formed by condensins are fundamental structuring principle These compact locally globally limit set chromatin self-repulsion. The length, density, increasingly structure observe fully explain chromosome emerges self-organization division.

Язык: Английский

Nanoscale DNA tracing reveals the self-organization mechanism of mitotic chromosomes DOI Creative Commons
Kai Sandvold Beckwith, Andreas Brunner, Natalia Rosalía Morero

и другие.

Cell, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

How genomic DNA is folded during cell division to form the characteristic rod-shaped mitotic chromosomes essential for faithful genome inheritance a long-standing open question in biology. Here, we use nanoscale tracing single dividing cells directly visualize how 3D fold of changes mitosis at scales from loops entire chromosomes. Our structural analysis reveals scaling minimum 6-8 megabases mitosis. Combined with data-driven modeling and molecular perturbations, can show that very large strongly overlapping formed by condensins are fundamental structuring principle These compact locally globally limit set chromatin self-repulsion. The length, density, increasingly structure observe fully explain chromosome emerges self-organization division.

Язык: Английский

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