Filamented Hydrogels as Tunable Conduits for Guiding Neurite Outgrowth DOI Creative Commons
Hao Liu, Anna Puiggalí‐Jou, Parth Chansoria

и другие.

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101471 - 101471

Опубликована: Янв. 11, 2025

Anisotropic scaffolds with unidirectionally aligned fibers present an optimal solution for nerve tissue engineering and graft repair. This study investigates the application of filamented light (FLight) biofabrication to create hydrogel matrices featuring highly microfilaments, facilitating neurite guidance outgrowth from encapsulated chicken dorsal root ganglion (DRG) cells. FLight employs optical modulation instability (OMI) rapidly safely (<5 s) fabricate constructs precise microfilament alignment. The tunability was demonstrated by adjusting four key parameters: stiffness, porosity, growth factor release, incorporation biological cues. Matrix stiffness fine-tuned varying projection dose, yielding ranging 0.6 5.7 kPa. Optimal occurred at a kPa, achieving 2.5 mm over 4 days. porosity modified using diffraction gratings in setup. While significant differences alignment were observed between bulk gels, further increases 40 % 70 enhanced cell migration axon bundling without significantly affecting maximal outgrowth. protein microcrystals containing (NGF) into photoresin enabled sustained need additional NGF media. Finally, laminin added resin enhance bioactivity biomaterial, resulting increase maximum 3.5 after days culture softer matrices. Overall, varied matrix properties achieved through outgrowth, highlighting importance adaptable scaffold characteristics guiding development. demonstrates potential as versatile platform creating ideal clinical applications repair engineering.

Язык: Английский

Filamented Hydrogels as Tunable Conduits for Guiding Neurite Outgrowth DOI Creative Commons
Hao Liu, Anna Puiggalí‐Jou, Parth Chansoria

и другие.

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101471 - 101471

Опубликована: Янв. 11, 2025

Anisotropic scaffolds with unidirectionally aligned fibers present an optimal solution for nerve tissue engineering and graft repair. This study investigates the application of filamented light (FLight) biofabrication to create hydrogel matrices featuring highly microfilaments, facilitating neurite guidance outgrowth from encapsulated chicken dorsal root ganglion (DRG) cells. FLight employs optical modulation instability (OMI) rapidly safely (<5 s) fabricate constructs precise microfilament alignment. The tunability was demonstrated by adjusting four key parameters: stiffness, porosity, growth factor release, incorporation biological cues. Matrix stiffness fine-tuned varying projection dose, yielding ranging 0.6 5.7 kPa. Optimal occurred at a kPa, achieving 2.5 mm over 4 days. porosity modified using diffraction gratings in setup. While significant differences alignment were observed between bulk gels, further increases 40 % 70 enhanced cell migration axon bundling without significantly affecting maximal outgrowth. protein microcrystals containing (NGF) into photoresin enabled sustained need additional NGF media. Finally, laminin added resin enhance bioactivity biomaterial, resulting increase maximum 3.5 after days culture softer matrices. Overall, varied matrix properties achieved through outgrowth, highlighting importance adaptable scaffold characteristics guiding development. demonstrates potential as versatile platform creating ideal clinical applications repair engineering.

Язык: Английский

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