Integrating Microarray Data and Single-Cell RNA-Seq Reveals Key Gene Involved in Spermatogonia Stem Cell Aging

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(21), С. 11653 - 11653

Опубликована: Окт. 30, 2024

The in vitro generation of spermatogonial stem cells (SSCs) from embryonic (ESCs) offers a viable approach for addressing male infertility. A multitude molecules participate this intricate process, which requires additional elucidation. Despite the decline SSCs aged testes, are deemed immortal since they can multiply three years with repeated transplantation. Nonetheless, examination aging is challenging due to limited quantity and absence precise indicators. Using microarray, we assessed genome-wide transcripts (about 55,000 transcripts) fibroblasts SSCs. WGCNA was then used look SSC-specific transcription factors (TFs) hub genes based on ATAC-seq, DNase-seq, RNA-seq, microarray data GEO databases as well gene expression (RNA-seq data). analysis human cases different revealed that 6 were upregulated, 23 downregulated compared normal case relation genes. To reach these results, online assessments Enrich Shiny GO, STRING, Cytoscape forecast molecular functional connections proteins before identifying master routes. biological process function keywords cell-matrix adhesion, telomerase activity, telomere cap complex shown be significantly altered upregulated differentially expressed (DEGs) by enrichment analysis. According our preliminary research, cell-specific TFs TF-mediated GRNs involved creation In order maximize induction efficiency ESC differentiation into vitro, important identified, sophisticated network regulation proposed. interact may able shine light pathophysiologies infertility aberrant germ cells.

Язык: Английский

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Withaferin-A induced Vimentin S56 phosphorylation dissociates NEDD9 signaling loop to regress progressive metastatic melanoma into lung adenocarcinoma.

Chemico-Biological Interactions, Год журнала: 2024, Номер unknown, С. 111319 - 111319

Опубликована: Ноя. 1, 2024

Язык: Английский

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Candidate oncogene placenta specific 8 affect cell growth and cell migration in non- small cell lung cancers

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Фев. 2, 2023

Abstract Background As a candidate oncogene, PLAC8 participate in genesis and progression of various tumors. However, the role lung cancer (LC) especially non-small cell (NSCLC) is still limited. Methods We performed Tissue microarray analysis (TMA) Real-Time PCR (RT-PCR) to detect expression levels LC tissues lines, respectively. Then series cellular experiments focusing on proliferation, cycle, motility were conducted identified NSCLC-derived lines H1299 A549. Results TMA RT-PCR showed that played complicated even opposite roles different LCs. Further confirmed could promote viability, alter accelerate mobility via regulation cyclins or cadherins, Conclusions Our study indicated might NSCLC progression. also shed new light potential as therapeutic biomarker NSCLC.

Язык: Английский

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Investigation of ZBTB16 Gene Expression by Bioinformatics Analysis and Immunocytochemistry and Immunohistochemistry Methods in Embryonic Cells Derived From Spermatogonial Stem Cells of Mouse Testis: A Laboratory Study

Journal of Rafsanjan University of Medical Sciences, Год журнала: 2023, Номер 22(9), С. 947 - 962

Опубликована: Дек. 1, 2023

Investigation of ZBTB16 Gene Expression by Bioinformatics Analysis and Immunocytochemistry Immunohistochemistry Methods in Embryonic Cells Derived From Spermatogonial Stem Mouse Testis: A Laboratory Study

Язык: Английский

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Candidate oncogene placenta specific 8 affects cell growth and cell migration in non-small cell lung cancers

Biocell, Год журнала: 2024, Номер 48(2), С. 239 - 252

Опубликована: Янв. 1, 2024

Язык: Английский

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