Study on the mechanism of KIF18B affecting the malignant progression of glioblastoma cells

Frontiers in Genetics, Год журнала: 2025, Номер 16

Опубликована: Март 5, 2025

Member of the driver protein family 18B (KIF18B) is a potential prognostic marker and highly expressed in variety cancers. However, its function glioblastoma (GBM) remains unclear. The expression data KIF18B were obtained by accessing TCGA, CGGA GEPIA databases, verified Western blot assay immunohistochemistry. Glioma RNA sequencing clinical information downloaded from TCGA Kaplan-plotter survival analysis Multivariable COX regression performed to plot ROC curves at 1, 3 5 years cBioPortal MethSurv used carefully examine value methylation. CBioPortal database UALCAN obtain co-expressed genes for GO KEGG enrichment analysis, gene set (GSEA) software was explore signaling pathway regulation GBM. Finally, correlation between GBM infiltration studied using TIMER dataset. various cancers including GBM, positively correlated with glioma grade negatively prognosis. curve showed that one independent risk factors methylation expression, overall rate patients hypomethylation lower than hypermethylation. A total 124 selected database. mainly involved malignant progression through P53 other pathways. GSEA high group enriched E2F, G2M results immunoassay immune tumor microenvironment. key factor affecting prognosis patients, targeting may provide new therapeutic method patients.

Язык: Английский

Decoding the Role of CDCA Genes in Breast Cancer Progression: Insights From in Silico and Functional Assay

Asia-Pacific Journal of Clinical Oncology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

ABSTRACT Background The cell division cycle‐associated (CDCA) genes regulate key cellular processes like cycle progression and division. This study evaluates the diagnostic clinical relevance of CDCA in breast cancer. Methodology Breast cancer normal lines were cultured analyzed for gene expression using RT‐qPCR further validated public databases. Functional assays, including proliferation, colony formation, wound healing, performed following siRNA‐mediated knockdown CDCA2 CDCA3. Mutational, CNV, methylation, survival analyses, along with miRNA regulation PPI network construction, conducted to explore role progression. Results Our findings revealed significant upregulation compared controls, all these exhibiting highest potential based on AUC values ROC analysis. Pathological stage analysis indicated that CDCA5 CDCA7 significantly varied across different stages. Mutational showed had mutation rate, missense mutations being most common. CNV amplification events several genes, particularly CDCA2, CDCA3, CDCA4, CDCA7. Promoter methylation hypomethylation cancer, which correlated negatively their expression. Survival demonstrated high CDCA5, CDCA7, CDCA8 was associated worse overall survival, highlighting prognostic significance. Furthermore, immune infiltration correlations between types, suggesting a modulation. identified specific miRNAs targeting showing as biomarkers. Lastly, CDCA3 cells reduced migration, indicating critical roles tumor growth metastasis. Conclusion highlights promising biomarkers Their correlates poor impairs growth, emphasizing therapeutic targets. These suggest could be integrated into practice improved management. Clinical trial number Not applicable.

Язык: Английский

Luteolin and glycyrrhetinic exert cooperative effect on liver cancer by selfassembling into carrier-free nanostructures

Chinese Chemical Letters, Год журнала: 2025, Номер unknown, С. 111325 - 111325

Опубликована: Май 1, 2025

Язык: Английский