Human Wound and Its Burden: Updated 2020 Compendium of Estimates

Advances in Wound Care, Год журнала: 2021, Номер 10(5), С. 281 - 292

Опубликована: Март 18, 2021

Significance: Chronic wounds impact the quality of life (QoL) nearly 2.5% total population in United States and management has a significant economic on health care. Given aging population, continued threat diabetes obesity worldwide, persistent problem infection, it is expected that chronic will continue to be substantial clinical, social, challenge. In 2020, coronavirus disease (COVID) pandemic dramatically disrupted care including wound A nonhealing (CNHW) typically correlated with comorbidities such as diabetes, vascular deficits, hypertension, kidney disease. These risk factors make persons CNHW at high for severe, sometimes lethal outcomes if infected severe acute respiratory syndrome 2 (pathogen causing COVID-19). The COVID-19 impacted several aspects continuum, compliance visits, prompting alternative approaches (use telemedicine creation videos help dressing changes among others), encouraging do-it-yourself protocol use homemade remedies/substitutions. Recent Advances: There developing interest understanding how social determinants QoL patients. Furthermore, addressing light highlighted importance options continuum Future Directions: economic, continues rise requires appropriate investment structured approach care, education, related research.

Язык: Английский

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Mesenchymal Stem/Stromal Cell-Derived Exosomes for Immunomodulatory Therapeutics and Skin Regeneration

Cells, Год журнала: 2020, Номер 9(5), С. 1157 - 1157

Опубликована: Май 7, 2020

Exosomes are nano-sized vesicles that serve as mediators for cell-to-cell communication. With their unique nucleic acids, proteins, and lipids cargo compositions reflect the characteristics of producer cells, exosomes can be utilized cell-free therapeutics. Among derived from various cellular origins, mesenchymal stem cell-derived (MSC-exosomes) have gained great attention due to immunomodulatory regenerative functions. Indeed, many studies shown anti-inflammatory, anti-aging wound healing effects MSC-exosomes in vitro vivo models. In addition, recent advances field exosome biology enabled development specific guidelines quality control methods, which will ultimately lead clinical application exosomes. This review highlights investigate therapeutic potential relevant mode actions skin diseases, well measures required exosome-derived

Язык: Английский

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Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing

Frontiers in Bioengineering and Biotechnology, Год журнала: 2020, Номер 8

Опубликована: Март 3, 2020

The cells secrete microvesicles that may have an endosomal origin, or from evaginations of the plasma membrane. former are referred to as exosomes, with sizes ranging 50 100 nm. These contain a lipid bilayer associated membrane proteins. Molecules such nucleic acids (DNA, mRNA, miRNA, lncRNA, etc) and proteins stored inside. exosome composition depends on producer cell-type its physiological conditions. Through them, modify their microenvironment behavior neighboring cells. That is accomplished by transferring factors modulate different metabolic signaling pathways. Due properties, exosomes can be applied diagnostic therapeutic tool in medicine. MSC haveimmunomodulatory properties high regenerative capacity. features linked paracrine activity microvesicle secretion. Therefore, research produced has been intensified for use cell-free In this area, treatment chronic skin ulcers (CSU) proposed. Such sores occur when normal healing does not resolve properly. usually due excessive prolongation inflammatory phase. aging diseases, diabetes, so prevalence increasing one latter disease, mainly developed countries. This very important socio-economic repercussions. review, we show application MSC-derived CSU positive effects, including accelerating decreasing scar formation. because immunosuppressive immunomodulatory properties. Likewise, they ability activate angiogenesis, proliferation, migration differentiation main cell-types involved regeneration. They include endothelial cells, fibroblasts keratinocytes. Most studies carried out far preclinical. there need advance more knowledge about conditions production, isolation action mechanisms exosomes. Interestingly, potential opens door design new highly effective strategies.

Язык: Английский

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Single cell transcriptomic landscape of diabetic foot ulcers

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Янв. 10, 2022

Abstract Diabetic foot ulceration (DFU) is a devastating complication of diabetes whose pathogenesis remains incompletely understood. Here, we profile 174,962 single cells from the foot, forearm, and peripheral blood mononuclear using single-cell RNA sequencing. Our analysis shows enrichment unique population fibroblasts overexpressing MMP1, MMP3, MMP11, HIF1A, CHI3L1 , TNFAIP6 increased M1 macrophage polarization in DFU patients with healing wounds. Further, spatially separated samples same patient spatial transcriptomics reveal preferential localization these associated toward wound bed as compared to edge or unwounded skin. Spatial also validates our findings higher abundance macrophages healers M2 non-healers. provides deep insights into microenvironment, identifying cell types that could be critical promoting healing, may inform novel therapeutic approaches for treatment.

Язык: Английский

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Electrospun Medicated Nanofibers for Wound Healing: Review

Membranes, Год журнала: 2021, Номер 11(10), С. 770 - 770

Опубликована: Окт. 9, 2021

With the increasing demand for wound care and treatment worldwide, traditional dressings have been unable to meet needs of existing market due their limited antibacterial properties other defects. Electrospinning technology has attracted more researchers’ attention as a simple versatile manufacturing method. The electrospun nanofiber membrane unique structure biological function similar extracellular matrix (ECM), is considered an advanced dressing. They significant potential in encapsulating delivering active substances that promote healing. This article first discusses common types dressing, then summarizes development fiber preparation technology. Finally, polymers biologically used electrospinning are summarized, portable equipment also discussed. Additionally, future research put forward.

Язык: Английский

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Fully automatic wound segmentation with deep convolutional neural networks

Scientific Reports, Год журнала: 2020, Номер 10(1)

Опубликована: Дек. 14, 2020

Acute and chronic wounds have varying etiologies are an economic burden to healthcare systems around the world. The advanced wound care market is expected exceed $22 billion by 2024. Wound professionals rely heavily on images image documentation for proper diagnosis treatment. Unfortunately lack of expertise can lead improper etiology inaccurate management documentation. Fully automatic segmentation areas in natural important part protocol since it crucial measure area provide quantitative parameters Various deep learning models gained success analysis including semantic segmentation. This manuscript proposes a novel convolutional framework based MobileNetV2 connected component labelling segment regions from images. advantage this model its lightweight less compute-intensive architecture. performance not compromised comparable deeper neural networks. We build annotated dataset consisting 1109 foot ulcer 889 patients train test models. demonstrate effectiveness mobility our method conducting comprehensive experiments analyses various full implementation available at https://github.com/uwm-bigdata/wound-segmentation .

Язык: Английский

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Dissolvable Microneedles Coupled with Nanofiber Dressings Eradicate Biofilms via Effectively Delivering a Database-Designed Antimicrobial Peptide

ACS Nano, Год журнала: 2020, Номер 14(9), С. 11775 - 11786

Опубликована: Авг. 25, 2020

Biofilms in chronic wounds, including diabetic foot ulcers, pressure and venous leg pose a major challenge to wound management. Herein, we report Janus-type antimicrobial dressing for eradication of biofilms wounds. The consists electrospun nanofiber membranes coupled with dissolvable microneedle arrays enable effective delivery database-designed peptide both inside outside biofilms. This exhibited high efficacy against broad spectrum resistant pathogens vitro. Importantly, such was able eradicate methicillin-resistant Staphylococcus aureus (MRSA) an ex vivo human skin infection model type II mouse after daily treatment without applying surgical debridement. Most importantly, the can also completely remove Pseudomonas aeruginosa MRSA, dual-species biofilm model. In addition, our computational simulations suggested that microneedles were more peptides than free drugs. Our results indicate dressings may provide management polymicrobial infections.

Язык: Английский

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Cutaneous Wound Healing: An Update from Physiopathology to Current Therapies

Life, Год журнала: 2021, Номер 11(7), С. 665 - 665

Опубликована: Июль 7, 2021

The skin is the biggest organ of human body which acts as a protective barrier against deleterious agents. When this damaged, organism promotes healing process with several molecular and cellular mechanisms, in order to restore physiological structure skin. control wound depends on correct balance among its different mechanisms. Any disruption these mechanisms can lead problems delay healing. impairment linked underlying factors well aging, nutrition, hypoxia, stress, infections, drugs, genetics, chronic diseases. Over years, numerous studies have been conducted discover approach best therapies for healing, including surgical procedures non-surgical treatments such topical formulations, dressings, or substitutes. Thus, general necessary facilitate direction further studies. This work provides updated concepts that interfere, used

Язык: Английский

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Engineering Platelet‐Rich Plasma Based Dual‐Network Hydrogel as a Bioactive Wound Dressing with Potential Clinical Translational Value

Advanced Functional Materials, Год журнала: 2020, Номер 31(8)

Опубликована: Ноя. 30, 2020

Abstract Delayed wound healing has a profound impact on patients, healthcare, and society. Platelet‐rich plasma (PRP) gel, as preparation for regenerative medicine, proven to be of clinical value in various treatments. Nevertheless, its weak mechanical properties consequent burst release effect have restricted application efficacy. Here, an engineered PRP dual‐network hydrogel (named DN gel) based sodium alginate is constructed through simple “one‐step” activation process. Its improved gelling property sustained growth factors may beneficial use. Evaluations rats indicate that gel promote terms rapid re‐epithelialization, up‐regulated factor levels early transitions the angiogenesis stages. As proof concept, also exhibits superior efficiency porcine model. These results demonstrate great potential transforming this into next generation PRP‐based bioactive dressing.

Язык: Английский

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Challenges in Healing Wound: Role of Complementary and Alternative Medicine

Frontiers in Nutrition, Год журнала: 2022, Номер 8

Опубликована: Янв. 20, 2022

Although the word wound sounds like a simple injury to tissue, individual's health status and other inherent factors may make it very complicated. Hence, healing has gained major attention in healthcare. The biology is precise highly programmed, through phases of hemostasis, inflammation, proliferation remodeling. Current options for which includes, use anti-microbial agents, promoters along with application herbal natural products. However, there no efficient evidence-based therapy available specific chronic wounds that can result definitive clinical outcomes. Under co-morbid conditions, would poses numerous challenges. Use Complementary Alternative Medicines (CAMs) care sector increasing its applications management remains "separate diamonds from ore." Attempts have been made understand at molecular level, mainly analysis signature genes influence several synthetic molecules on these. We outlined review challenges role CAMs management. main focus limitations currently treatment non-healing best possible alternates consider. This information generates broader knowledge healing, be further addressed using multidisciplinary approach combination therapies.

Язык: Английский

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