Journal of Engineering and Science in Medical Diagnostics and Therapy,
Год журнала:
2023,
Номер
7(1)
Опубликована: Июнь 10, 2023
Abstract
To
investigate
the
effect
of
shear
stress
direction
on
cell
activity
in
a
confluent
layer,
migration
and
deformation
cells
oriented
parallel
or
perpendicular
to
flow
field
were
optically
tracked
vitro.
A
Couette-type
between
walls
was
formed
lower
stationary
culture
dish
upper
rotating
disk.
Shear
(<2
Pa)
set
by
adjusting
rotational
speed
Myoblasts
(C2C12:
mouse
myoblast
line)
cultured
an
incubator
equipped
with
inverted
phase-contrast
microscope
under
continuous
for
7
days
until
confluency.
Deformation
each
time-lapse
images.
Analysis
these
images
showed
that
deform
migrate
along
their
major
axis
even
at
confluency
(whether
is
field).
As
result,
orientation
remains
field.
This
observation
may
be
used
improve
development
engineered
muscle
tissue.
Disease Models & Mechanisms,
Год журнала:
2023,
Номер
16(10)
Опубликована: Окт. 1, 2023
ABSTRACT
The
endothelium,
the
monolayer
of
endothelial
cells
that
line
blood
vessels,
is
exposed
to
a
number
mechanical
forces,
including
frictional
shear
flow,
pulsatile
stretching
and
changes
in
stiffness
influenced
by
extracellular
matrix
composition.
These
forces
are
sensed
mechanosensors
facilitate
their
transduction
drive
appropriate
adaptation
endothelium
maintain
vascular
homeostasis.
In
aorta,
unique
architecture
vessel
gives
rise
fluid
dynamics,
which,
turn,
shape
cellular
morphology,
nuclear
architecture,
chromatin
dynamics
gene
regulation.
this
Review,
we
discuss
recent
work
focusing
on
how
differential
exerted
transduced
influence
form
function
giving
spatial
variation
aorta.
We
will
also
developments
understanding
mechanosensing
implicated
diseases
Silk
fibroin
(SF),
which
is
extensively
utilized
in
tissue
engineering
and
vascular
grafts
for
enhancing
regeneration,
has
not
been
thoroughly
investigated
its
epigenetic
effects
on
endothelial
cells
(EC).
This
study
employed
RNA
sequencing
analysis
to
evaluate
the
activation
of
histone
modification
regulatory
genes
EC
treated
with
SF.
Subsequent
investigations
revealed
elevated
H3K9me3
levels
SF-treated
EC,
as
evidenced
by
immunofluorescence
western
blot
analysis.
The
H2B-eGFP
demonstrate
that
SF
treatment
results
accumulation
H2B-marked
chromatin
nuclear
inner
cavities
EC.
Inhibition
a
deacetylase
inhibitor
TSA
decreased
cell
proliferation.
Furthermore,
MAPK
signaling
pathway
using
chromium
picolinate
proliferative
activity
level
also
appeared
enhance
growth
proliferation
modulating
reorganizing
chromatin,
particularly
after
oxidative
stress
induced
H
Frontiers in Cardiovascular Medicine,
Год журнала:
2022,
Номер
9
Опубликована: Дек. 7, 2022
Endothelial-mesenchymal
transition
(EndMT)
is
a
differentiation
process
in
which
endothelial
cells
lose
their
own
characteristics
and
acquire
mesenchymal-like
characteristics,
contributes
to
the
formation
development
of
atherosclerotic
plaques.
Until
now,
there
still
lack
effective
measures
treat
atherosclerosis
(AS),
so
an
urgent
need
understand
underlying
mechanisms
AS.
In
addition,
although
various
studies
have
shown
that
EndMT
involved
pathological
stages
cardiovascular
diseases,
such
as
myocardial
fibrosis,
hypertrophy,
hypertension,
specific
molecular
driving
are
exploratory
stage.
this
review,
we
review
role
histone
modifications
(methylation,
demethylation
acetylation,
deacetylation)
on
disease,
aiming
target
histone-modifying
enzymes
guide
disease
therapy.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 28, 2023
Abstract
Early
atherosclerotic
lesions
often
develop
in
areas
of
disturbed
flow
within
arterial
curvatures
and
bifurcations.
Complex
flows
alter
the
signaling
inflammatory
other
molecules
that
may
exacerbate
disease
phenotype.
Microfluidic
platforms
to
assess
changes
endothelial
mechanobiology
generally
employ
laminar
unidirectional
or
oscillatory
generated
straight
channels;
such
do
not
however
mimic
complex
time-varying
bi-directional
shear
patterns
on
walls.
We
fabricated
an
endothelium-on-chip
device
generate
“controlled”
flows,
characterized
using
a
wall
rosette,
as
those
reported
aneurysmal
vessels
regions
with
plaques.
cultured
human
aortic
cells
(HAEC)
subjected
monolayer
circular
rosette
which
represents
bidirectional
stresses.
Immunofluorescence
results
show
large
cuboidal
significantly
higher
nuclear
areas,
localization
VE-Cadherin,
elevated
actin
NF-kB
expressions
compared
cell
monolayers
flow,
No-flow
conditions.
The
creation
dysfunctional
due
also
correlated
dramatic
lamin
A/C
distribution
heterochromatin
organization
nucleus
have
been
earlier.
Such
studies
are
potentially
useful
novel
therapeutics,
mitigate
effects
vascular
for
personalized
medicine,
study
thrombus
vicinity
inflamed
monolayers,
reduce
ourreliance
animal
trials.
Our
analytical
method
represent
first
successful
demonstration
generating
controlled
microfluidic
devices
Frontiers in Physiology,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 22, 2024
Hutchinson-Gilford
Progeria
Syndrome
(HGPS)
is
a
premature
aging
disorder
that
causes
severe
cardiovascular
disease,
resulting
in
the
death
of
patients
their
teenage
years.
The
disease
pathology
caused
by
accumulation
progerin,
mutated
form
nuclear
lamina
protein,
lamin
A.
Progerin
binds
to
inner
membrane,
disrupting
integrity,
and
abnormalities
changes
gene
expression.
This
results
increased
cellular
inflammation,
senescence,
overall
dysfunction.
molecular
mechanisms
which
progerin
induces
are
not
fully
understood.
Progerin’s
detrimental
impact
on
mechanics
role
nucleus
as
mechanosensor
suggests
dysfunctional
mechanotransduction
could
play
HGPS.
especially
relevant
cells
exposed
dynamic,
continuous
mechanical
stimuli,
like
those
vasculature.
endothelial
(ECs)
smooth
muscle
(SMCs)
within
arteries
rely
physical
forces
produced
blood
flow
maintain
function
homeostasis.
Certain
regions
produce
disturbed
flow,
leading
an
impaired
transduction
signals,
reduction
function,
also
occurs
In
this
review,
we
discuss
mechanotransduction,
how
disrupted
HGPS,
what
effect
has
cell
health
function.
We
address
healthy
responses
ECs
SMCs
physiological
stimuli
these
accumulation.
Journal of Engineering and Science in Medical Diagnostics and Therapy,
Год журнала:
2023,
Номер
7(1)
Опубликована: Июнь 10, 2023
Abstract
To
investigate
the
effect
of
shear
stress
direction
on
cell
activity
in
a
confluent
layer,
migration
and
deformation
cells
oriented
parallel
or
perpendicular
to
flow
field
were
optically
tracked
vitro.
A
Couette-type
between
walls
was
formed
lower
stationary
culture
dish
upper
rotating
disk.
Shear
(<2
Pa)
set
by
adjusting
rotational
speed
Myoblasts
(C2C12:
mouse
myoblast
line)
cultured
an
incubator
equipped
with
inverted
phase-contrast
microscope
under
continuous
for
7
days
until
confluency.
Deformation
each
time-lapse
images.
Analysis
these
images
showed
that
deform
migrate
along
their
major
axis
even
at
confluency
(whether
is
field).
As
result,
orientation
remains
field.
This
observation
may
be
used
improve
development
engineered
muscle
tissue.