The mechanical influence of densification on epithelial architecture
PLoS Computational Biology,
Год журнала:
2024,
Номер
20(4), С. e1012001 - e1012001
Опубликована: Апрель 1, 2024
Epithelial
tissues
are
the
most
abundant
tissue
type
in
animals,
lining
body
cavities
and
generating
compartment
barriers.
The
function
of
a
monolayered
epithelial
tissue–whether
protective,
secretory,
absorptive,
or
filtrative–relies
on
side-by-side
arrangement
its
component
cells.
mechanical
parameters
that
determine
shape
cells
apical-basal
plane
not
well-understood.
architecture
culture
is
intimately
connected
to
cell
density,
cultured
layers
transition
between
architectures
as
they
proliferate.
This
prompted
us
ask
what
extent
emerges
from
two
considerations:
A)
constraints
densification
B)
cell-cell
adhesion,
hallmark
feature
To
address
these
questions,
we
developed
novel
polyline
cell-based
computational
model
used
it
make
theoretical
predictions
about
upon
changes
density
adhesion.
We
tested
using
experiments.
Our
results
show
appearance
extended
lateral
borders
arises
consequence
crowding–independent
However,
cadherin-mediated
adhesion
associated
with
architectural
transition.
suggest
this
represents
initial
distinctive
architecture.
Together
our
work
reveals
distinct
roles
layer
formation
provides
framework
understand
less
well-studied
tissues.
Язык: Английский
Study Models for Chlamydia trachomatis Infection of the Female Reproductive Tract
Microorganisms,
Год журнала:
2025,
Номер
13(3), С. 553 - 553
Опубликована: Фев. 28, 2025
Chlamydia
trachomatis
(Ct)
is
a
leading
cause
of
sexually
transmitted
infections
globally,
often
resulting
in
inflammatory
disorders,
ectopic
pregnancies,
and
infertility.
Studying
Ct’s
pathogenesis
remains
challenging
due
to
its
unique
life
cycle
host-specific
interactions,
which
require
diverse
experimental
models.
Animal
studies
using
mouse,
guinea
pig,
non-human
primate
models
provide
valuable
insights
into
immune
responses,
hormonal
influences,
disease
progression.
However,
they
face
limitations
terms
translational
relevance
physiological
differences,
as
well
ethical
concerns.
Complementing
these,
vitro
systems,
ranging
from
simple
monolayer
advanced
three-dimensional
models,
exhibit
improved
by
replicating
the
human
tissue
architecture.
This
includes
detailed
investigation
epithelial
barrier
disruptions,
epithelium–stroma
responses
at
cellular
level.
Nonetheless,
fall
short
mimicking
intricate
structures
found
vivo
and,
therefore,
cannot
faithfully
replicate
host–pathogen
interactions
or
infection
dynamics
observed
living
organisms.
review
presents
comprehensive
overview
employed
over
past
few
decades
investigate
Ct
pathogenesis,
addressing
their
strengths
limitations.
Furthermore,
we
explore
emerging
technologies,
including
organ-on-chip
silico
promising
tools
overcome
existing
challenges
refine
our
understanding
infections.
Язык: Английский
A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Май 29, 2024
Epithelial
tissue
forms
and
maintains
a
critical
barrier
function
in
the
body.
A
novel
culture
design
aimed
at
promoting
uniform
maturation
of
epithelial
cells
using
liquid
materials
is
described.
Culturing
Madin-Darby
canine
kidney
(MDCK)
liquid-liquid
interface
yielded
reduced
migration
stimulated
active
cell
growth.
Similar
to
solid-liquid
interfaces,
cultured
on
fibronectin-coated
exhibited
growth,
ultimately
reaching
confluent
state.
These
stress
fiber
formation
adopted
cobblestone-like
shape,
which
led
their
even
distribution
vessel.
To
inhibit
apoptosis,
exposure
interfaces
Y27632,
specific
inhibitor
Rho-associated
protein
kinase
(ROCK),
facilitated
tight
junction
(frequency
ZO-2-positive
cells,
FZ
=
0.73).
In
Y27632-exposed
interface,
value
obtained
by
subtracting
standard
deviation
ratio
nucleus
densities
each
region
that
compartmentalized
vessel
from
1,
denoted
as
HLN,
was
0.93
±
0.01,
indicated
t
72
h.
The
behavior
contributes
promotion
maturation.
Язык: Английский
A novel strategy to facilitate uniform epithelial cell maturation on liquid-liquid interfaces
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 8, 2024
Abstract
Background:
Liquid-liquid
interfaces,
such
as
lacrimal
fluid
of
the
eye,
are
pervasive
in
living
organism.
While
culturing
cells
an
environment
that
closely
mimics
vivo
conditions
enhances
our
understanding
cell
characteristics
and
unveils
underlying
mechanisms,
practical
considerations
have
led
to
predominant
use
solid
surfaces,
polystyrene
or
glass,
for
culture.
In
this
study,
we
introduce
a
novel
culture
design
aimed
at
promoting
uniform
maturation
epithelial
using
liquid
materials.
Results:
Culturing
Madin–Darby
canine
kidney
(MDCK)
liquid-liquid
interface
yielded
reduced
migration
stimulated
active
growth.
Similar
solid-liquid
cultured
on
fibronectin-coated
exhibited
growth,
ultimately
reaching
confluent
state.
These
stress
fiber
formation
adopted
cobblestone-like
shape,
which
their
even
distribution
vessel.
Analysis
staining
images
tight
junction
(TJ)
protein
ZO-2
revealed
frequency
ZO-2-positive
cells,
F
Z
,
cultures
was
2.2-fold
higher
than
non-coated
t
=
72
h.
To
inhibit
apoptosis,
exposure
Y27632,
specific
inhibitor
Rho-associated
kinase
(ROCK),
facilitated
(
0.73).
Additionally,
similar
trend
observed
0.80)
Y27632-exposed
interface,
value
obtained
by
subtracting
standard
deviation
ratio
nucleus
densities
each
region
compartmentalized
vessel
from
1,
denoted
H
LN
0.93
0.01,
indicating
Across
all
conditions,
values
close
correlation
R
2
0.93),
suggesting
is
related
TJs
late
phase
maturation.
Conclusions:
Our
findings
suggest
behaviors
interfaces
contribute
promotion
Язык: Английский
A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 28, 2024
Abstract
Epithelial
tissue
forms
and
maintains
a
critical
barrier
function
in
the
body.
A
novel
culture
design
aimed
at
promoting
uniform
maturation
of
epithelial
cells
using
liquid
materials
is
described.
Culturing
Madin–Darby
canine
kidney
(MDCK)
liquid–liquid
interface
yielded
reduced
migration
stimulated
active
cell
growth.
Similar
to
solid–liquid
interfaces,
cultured
on
fibronectin-coated
exhibited
growth,
ultimately
reaching
confluent
state.
These
stress
fiber
formation
adopted
cobblestone-like
shape,
which
led
their
even
distribution
vessel.
To
inhibit
apoptosis,
exposure
interfaces
Y27632,
specific
inhibitor
Rho-associated
protein
kinase
(ROCK),
facilitated
tight
junction
(frequency
ZO-2-positive
cells,
F
Z
=
0.73).
In
Y27632-exposed
interface,
value
obtained
by
subtracting
standard
deviation
ratio
nucleus
densities
each
region
that
compartmentalized
vessel
from
1,
denoted
as
H
LN
,
was
0.93
±
0.01,
indicated
t
72
h.
The
behavior
contributes
promotion
maturation.
Язык: Английский
A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 5, 2024
Abstract
Epithelial
tissue
forms
and
maintains
a
critical
barrier
function
in
the
body.
A
novel
culture
design
aimed
at
promoting
uniform
maturation
of
epithelial
cells
using
liquid
materials
is
described.
Culturing
Madin–Darby
canine
kidney
(MDCK)
liquid–liquid
interface
yielded
reduced
migration
stimulated
active
cell
growth.
Similar
to
solid–liquid
interfaces,
cultured
on
fibronectin-coated
exhibited
growth,
ultimately
reaching
confluent
state.
These
stress
fiber
formation
adopted
cobblestone-like
shape,
which
led
their
even
distribution
vessel.
To
inhibit
apoptosis,
exposure
interfaces
Y27632,
specific
inhibitor
Rho-associated
protein
kinase
(ROCK),
facilitated
tight
junction
(frequency
ZO-2-positive
cells,
F
Z
=
0.73).
In
Y27632-exposed
interface,
value
obtained
by
subtracting
standard
deviation
ratio
nucleus
densities
each
region
that
compartmentalized
vessel
from
1,
denoted
as
H
LN
,
was
0.93
0.01,
indicated
t
72
h.
The
behavior
contributes
promotion
maturation.
Язык: Английский
A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 6, 2024
Abstract
Epithelial
tissue
forms
and
maintains
a
critical
barrier
function
in
the
body.
A
novel
culture
design
aimed
at
promoting
uniform
maturation
of
epithelial
cells
using
liquid
materials
is
described.
Culturing
Madin–Darby
canine
kidney
(MDCK)
liquid–liquid
interface
yielded
reduced
migration
stimulated
active
cell
growth.
Similar
to
solid–liquid
interfaces,
cultured
on
fibronectin-coated
exhibited
growth,
ultimately
reaching
confluent
state.
These
stress
fiber
formation
adopted
cobblestone-like
shape,
which
led
their
even
distribution
vessel.
To
inhibit
apoptosis,
exposure
interfaces
Y27632,
specific
inhibitor
Rho-associated
protein
kinase
(ROCK),
facilitated
tight
junction
(frequency
ZO-2-positive
cells,
F
Z
=
0.73).
In
Y27632-exposed
interface,
value
obtained
by
subtracting
standard
deviation
ratio
nucleus
densities
each
region
that
compartmentalized
vessel
from
1,
denoted
as
H
LN
,
was
0.93
0.01,
indicated
t
72
h.
The
behavior
contributes
promotion
maturation.
Язык: Английский
Automated Layer Analysis (ALAn): An Image Analysis Tool for the Unbiased Characterization of Mammalian Epithelial Architecture in Culture
BIO-PROTOCOL,
Год журнала:
2024,
Номер
14(1343)
Опубликована: Янв. 1, 2024
Cultured
mammalian
cells
are
a
common
model
system
for
the
study
of
epithelial
biology
and
mechanics.
Epithelia
often
considered
as
pseudo-two
dimensional
thus
imaged
analyzed
with
respect
to
apical
tissue
surface.
We
found
that
three-dimensional
architecture
monolayers
can
vary
widely
even
within
small
culture
wells,
layers
appear
organized
in
plane
show
gross
disorganization
apical-basal
plane.
Epithelial
cell
shapes
should
be
3D
understand
maturity
cultured
accurately
compare
between
experiments.
Here,
we
present
detailed
protocol
use
our
image
analysis
pipeline,
Automated
Layer
Analysis
(ALAn),
developed
quantitatively
characterize
layers.
ALAn
is
based
on
set
rules
applied
spatial
distributions
DNA
actin
signals
(depth)
dimension
obtained
from
imaging
using
confocal
microscope.
facilitates
reproducibility
across
experiments,
investigations,
labs,
providing
users
quantitative,
unbiased
characterization
maturity.
Key
features
•
This
was
spatially
analyze
an
automated
fashion.
requires
two
inputs:
nuclei
fluorescence
microscopy.
code
written
Python3
Jupyter
Notebook
interactive
format.
Optimized
Marbin-Darby
Canine
Kidney
(MDCK)
successfully
human
MCF-7
mammary
gland-derived
Caco-2
colon
carcinoma
cells.
utilizes
Imaris
software
segment
but
may
adapted
alternative
method.
centroid
coordinates
volume
nuclei.
Язык: Английский
The Mechanical Influence of Densification on Initial Epithelial Architecture
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Май 9, 2023
Summary
Epithelial
tissues
are
the
most
abundant
tissue
type
in
animals,
lining
body
cavities
and
generating
compartment
barriers.
The
function
of
a
monolayer
epithelium
–
whether
protective,
secretory,
absorptive,
or
filtrative
–relies
on
regular
architecture
with
respect
to
apical-basal
axis.
Using
an
unbiased
3D
analysis
pipeline
developed
our
lab,
we
previously
showed
that
epithelial
architectures
culture
can
be
divided
into
distinct
developmental
categories,
these
intimately
connected
cell
density:
at
sparse
densities,
cultured
layers
have
squamous
morphology
(Immature);
intermediate
develop
lateral
cell-cell
borders
rounded
apices
(Intermediate);
cells
highest
densities
reach
their
full
height
demonstrate
flattened
(Mature).
These
observations
prompted
us
ask
emerges
from
mechanical
constraints
densification,
what
extent
hallmark
feature
cells,
namely
adhesion,
contributes.
In
other
words,
is
shape
layer
simple
matter
sticky,
deformable
objects
squeezing
together?
We
addressed
this
problem
using
combination
computational
modeling
experimental
manipulations.
Our
results
show
first
morphological
transition,
Immature
Intermediate,
explained
simply
by
crowding.
Additionally,
identify
new
division
(and
thus
transition)
within
Intermediate
category,
find
second
relies
adhesion.
Язык: Английский