Dietary amino acids promote glucagon-like hormone release to generate global calcium waves in adipose tissues in Drosophila

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 2, 2025

Propagation of intercellular calcium waves through tissues has been found to coordinate different multicellular responses. Nevertheless, our understanding how operate remains limited. In this study, we explore the real-time dynamics in Drosophila adipose tissues. We identify Adipokinetic Hormone (AKH), fly functional homolog glucagon, as key factor driving Ca2+ activities tissue. find that AKH, which is released into hemolymph from AKH-producing neurosecretory cells, stimulates larval fat by a previously unrecognized gap-junction-independent mechanism promote lipolysis. adult body, however, gap-junction-dependent are triggered presumably uniformly diffused AKH. Additionally, discover amino acids activate leading increased intracellular and AKH secretion. Altogether, show dietary regulate release cells brain, subsequently tissue, enhancing lipid metabolism. Intercellular (ICWs) responses, although precise mechanisms less understood. Here, authors nutrient-triggered hormone brain ICWs

Язык: Английский

Calcium dynamics of skin-resident macrophages during homeostasis and tissue injury

Molecular Biology of the Cell, Год журнала: 2024, Номер 35(12)

Опубликована: Ноя. 13, 2024

Immune cells depend on rapid changes in intracellular calcium activity to modulate cell function. Skin contains diverse immune types and is critically dependent signaling for homeostasis repair, yet the dynamics functions of skin remain poorly understood. Here, we characterize Langerhans cells, skin-resident macrophages responsible surveillance clearance cellular debris after tissue damage. reside epidermis extend dynamic dendrites close proximity adjacent keratinocytes somatosensory peripheral axons. We find that homeostatic exhibit spontaneous transient activity, with flux occurring primarily body rarely dendrites. Triggering axon degeneration increases frequency By contrast, show a sustained increase following engulfment damaged keratinocytes. Altering leads decrease efficiency keratinocyte debris. Our findings demonstrate context-specific highlight utility as an accessible model imaging tissue-resident macrophages. [Media: see text] text].

Язык: Английский

Calcium dynamics of skin-resident macrophages during homeostasis and tissue injury

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 26, 2024

ABSTRACT Immune cells depend on rapid changes in intracellular calcium activity to modulate cell function. Skin contains diverse immune types and is critically dependent signaling for homeostasis repair, yet the dynamics functions of skin remain poorly understood. Here, we characterize Langerhans cells, skin-resident macrophages responsible surveillance clearance cellular debris after tissue damage. reside epidermis extend dynamic dendrites close proximity adjacent keratinocytes somatosensory peripheral axons. We find that homeostatic exhibit spontaneous transient activity, with flux occurring primarily body rarely dendrites. Triggering axon degeneration increases frequency By contrast, show a sustained increase following engulfment damaged keratinocytes. Altering leads decrease efficiency keratinocyte debris. Our findings demonstrate context-specific highlight utility as an accessible model imaging tissue-resident macrophages. SIGNIFICANCE STATEMENT Calcium thought regulate function, but studies focusing are limited. Skin-resident known conditions, which can change depending type injury inflicted. Pharmacological perturbation engulfment. These suggest important by cells.

Язык: Английский