NCAM1-SHIP2 axis upon recognizing microbes inhibits the expressions of inflammatory factors through P38-H3K4me and P38-NF-κB pathways in oyster
Cell Communication and Signaling,
Год журнала:
2025,
Номер
23(1)
Опубликована: Фев. 20, 2025
Neural
cell
adhesion
molecule
1
(NCAM1/CD56)
as
a
well-known
surface
marker
for
natural
killer
(NK)
cells
plays
important
roles
in
migration,
adhesion,
and
inflammation.
In
the
present
study,
NCAM1
homolog
containingthree
immunoglobulin
domains,
one
fibronectin
type
3
domain,
transmembrane
region
cytoplasmic
tail
with
two
intracellular
immunoreceptor
tyrosine-based
inhibitory
motifs
(ITIMs)
was
identified
from
Pacific
oyster,
Crassostrea
gigas
(defined
CgNCAM1).
The
mRNA
transcripts
of
CgNCAM1
were
highly
expressed
haemocytes.
expressions
haemocytes
increased
significantly
after
Vibrio
splendidus
stimulation.
positive
green
signals
SH2-containing
inositol
5-phosphatase
(CgSHIP2)
could
translocate
onto
haemocyte
membrane
V.
recombinant
extracellular
domains
exhibited
binding
activity
towards
various
pathogen-associated
molecular
patterns
(PAMPs)
microbes.
Upon
to
its
ligands,
recruited
CgSHIP2
transduce
inhibitor
reduce
phosphorylation
CgP38.
inhibition
CgP38
reduced
methylation
histone
H3K4
nuclear
translocation
NF-κB,
which
eventually
inhibited
inflammatory
factors
(CgIL17-2/3/6
CgTNF-2)
suppress
These
results
suggested
that
function
an
immune
checkpoint
sense
different
PAMPs
microbes
inflammation
through
inhibiting
P38-epigenetic
P38-NF-κB
pathways
oysters.
Язык: Английский
The single-cell atlas of short-chain fatty acid receptors in human and mice hearts
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 16, 2025
The
gut
microbiota
metabolite,
short-chain
fatty
acids
(SCFAs),
can
protect
against
multiple
cardiovascular
diseases,
while
the
molecular
targets
and
underlying
mechanisms
need
to
be
elucidated.
One
of
primary
SCFA
benefits
was
direct
activation
a
group
G-protein-coupled
receptors
(GPCRs),
termed
free
acid
(FFARs),
FFAR2
(GPR43),
FFAR3
(GPR41).
At
present,
distribution
FFAR2/3
in
cardiac
cells
has
not
been
entirely
clarified.
Using
18
public
single-cell
RNA-seq
single-nuclear
data
human
mouse
hearts,
we
illustrate
entire
atlas
different
regions
cell
types
normal
infarcted
hearts.
We
present
whole
body,
hearts
at
resolution.
also
illustrated
normal/ischemic
newborn
adult
mice
by
combining
newly
built
sc/snRNA-seq
datasets.
These
findings
provide
valuable
information
on
possible
effect
SCFAs
via
heart
references
for
future
studies.
Язык: Английский
CD56 on intratumoral NK cells: orchestrating NK cell-mediated anti-tumor effects in bladder cancer
Neoplasia,
Год журнала:
2025,
Номер
66, С. 101187 - 101187
Опубликована: Май 28, 2025
Язык: Английский
CD56 does not contribute to the anti-tumor, tissue homing, and glycolytic capacity of human NK cells
Journal of Leukocyte Biology,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 25, 2024
Abstract
Natural
killer
(NK)
cells
are
critical
innate
immune
involved
in
the
clearance
of
virally
infected
and
malignant
cells.
Human
NK
distinguished
by
their
surface
expression
CD56
a
lack
CD3.
While
cell
density
has
long
been
used
as
prototypic
marker
to
characterize
primary
human
functional
subsets,
exact
role
is
still
not
fully
understood.
Here,
we
eliminated
ex
vivo
expanded
(CD56bright)
using
CRISPR/Cas9
order
assess
function
this
highly
activated
cytotoxic
population.
We
show
that
no
effect
on
proliferative
capacity
or
various
activation
inhibitory
markers.
Further,
does
contribute
cell–mediated
cytotoxicity,
inflammatory
cytokine
production,
ability
control
tumor
engraftment
vivo.
also
found
while
deletion
did
impact
glycolytic
metabolism,
it
increase
reliance
oxidative
phosphorylation.
Last,
alter
tissue
trafficking.
Our
results
indicate
could
be
hyperfunctional
state
cells,
directly
influence
antitumor
functions
Язык: Английский
Extracellular vesicles epitopes as potential biomarker candidates in patients with traumatic spinal cord injury
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 27, 2024
Background
Extracellular
vesicles
(EVs),
a
heterogeneous
group
of
cell-derived,
membrane-enclosed
bearing
cell-specific
epitopes,
have
been
demonstrated
to
play
crucial
role
in
neuronal-glial
communication
and
the
orchestration
neuroinflammatory
processes.
However,
existing
evidence
regarding
their
function
as
biomarkers
pathobiology
traumatic
spinal
cord
injuries
(tSCI),
particularly
humans,
is
scarce.
Objective
The
primary
goal
this
study
was
investigate
whether
distinct
pattern
EV
surface
epitopes
detected
plasma
individuals
suffering
from
injury
indicative
tSCI.
Methods
includes
patients
with
isolated
tSCI
(n=8),
polytrauma
without
(PT;
ISS
≥16,
n=8),
healthy
volunteers
(HV;
n=8).
Plasma
samples
PT
were
collected
right
after
admission
emergency
room
(ER),
24
hours
(24h),
48h
trauma.
EVs
via
size
exclusion
chromatography,
EVs’
quantified
MACSPlex
Kit
Neuro
(prototype
product,
Miltenyi
Biotec)
compared
among
groups.
Additionally,
results
correlated
clinical
parameters.
Results
In
total,
19
differed
significantly
between
HV
Out
these
19,
four
(CD47,
CD56,
CD68,
ADAM17)
found
differ
expression
CD47
epitope
correlate
positively
American
Spinal
Injury
Association
(ASIA)
impairment
scale.
Conclusion
We
identified
potential
EV-based
(CD47+,
CD56+,
CD68+,
ADAM17+
EVs)
that
tSCI,
CD47+
showing
strong
correlation
neurological
Thus,
future
studies
might
further
specify
relevance
tSCI-specific
underlying
mechanisms
Язык: Английский