A mechanism for MEX-5-driven disassembly of PGL-3/RNA condensates in vitro DOI Creative Commons

Natasha S. Lewis,

Silja Zedlitz, Hannes Ausserwöger

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(20)

Опубликована: Май 12, 2025

MEX-5 regulates the formation and dissolution of P granules in Caenorhabditis elegans embryos, yet thermodynamic basis its activity remains unclear. Here, using a time-resolved vitro reconstitution system, we show that dissolves preassembled liquid-like PGL-3/RNA condensates by altering RNA availability shifting phase boundary. We develop microfluidic assay to systematically analyze how influences separation. By measuring contribution PGL-3 separation, reduces free energy PGL-3, equilibrium toward dissolution. Our findings provide quantitative framework for understanding RNA-binding proteins modulate condensate stability demonstrate power microfluidics precisely mapping transitions.

Язык: Английский

Novel Reporter Constructs to Accelerate Antiviral and Therapeutic Discovery for Enterovirus-A71 DOI Creative Commons
William Bakhache,

Ann Q. Shen,

Walker E. Orr

и другие.

Antiviral Research, Год журнала: 2025, Номер 235, С. 106094 - 106094

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Emerging frontiers in protein structure prediction following the AlphaFold revolution DOI Creative Commons
Martin L. Rennie, Michael R. Oliver

Journal of The Royal Society Interface, Год журнала: 2025, Номер 22(225)

Опубликована: Апрель 1, 2025

Models of protein structures enable molecular understanding biological processes. Current structure prediction tools lie at the interface biology, chemistry and computer science. Millions models have been generated in a very short space time through revolution driven by deep learning, led AlphaFold. This has provided wealth new structural information. Interpreting these predictions is critical to determining where when this information useful. But proteins are not static nor do they act alone, interacting with other biomolecules complete their function level. review focuses on application state-of-the-art advanced applications. We also suggest set guidelines for reporting AlphaFold predictions.

Язык: Английский

Процитировано

0
A mechanism for MEX-5-driven disassembly of PGL-3/RNA condensates in vitro DOI Creative Commons

Natasha S. Lewis,

Silja Zedlitz, Hannes Ausserwöger

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(20)

Опубликована: Май 12, 2025

MEX-5 regulates the formation and dissolution of P granules in Caenorhabditis elegans embryos, yet thermodynamic basis its activity remains unclear. Here, using a time-resolved vitro reconstitution system, we show that dissolves preassembled liquid-like PGL-3/RNA condensates by altering RNA availability shifting phase boundary. We develop microfluidic assay to systematically analyze how influences separation. By measuring contribution PGL-3 separation, reduces free energy PGL-3, equilibrium toward dissolution. Our findings provide quantitative framework for understanding RNA-binding proteins modulate condensate stability demonstrate power microfluidics precisely mapping transitions.

Язык: Английский

Процитировано

0