Microbiology Spectrum,
Год журнала:
2024,
Номер
12(4)
Опубликована: Март 1, 2024
ABSTRACT
Pasteurella
multocida
is
an
upper
respiratory
tract
commensal
in
several
mammal
and
bird
species
but
can
also
cause
severe
disease
humans
production
animals
such
as
poultry,
cattle,
pigs.
In
this
study,
we
performed
whole-genome
sequencing
of
P.
isolates
recovered
from
a
range
human
infections,
the
mouths
cats,
wounds
on
dogs.
Together
with
publicly
available
genome
sequences,
phylogenetic
comparative
genomic
analyses.
While
cats
dogs
were
spread
across
tree,
infections
caused
almost
exclusively
by
subsp.
septica
strains.
Most
capsule
type
A
LPS
L1
L3;
however,
some
strains
lacked
biosynthesis
locus,
contained
novel
outer-core
distinct
eight
loci
that
currently
be
identified
using
multiplex
PCR.
addition,
isolated
mobile
genetic
elements.
We
compiled
curated
database
known
virulence
factor
antibiotic
resistance
genes
(PastyVRDB)
allowing
for
detailed
characterization
isolates.
The
majority
encoded
reduced
iron
receptors
only
one
filamentous
hemagglutinin
gene.
Finally,
gene-trait
analysis
putative
L-fucose
uptake
utilization
pathway
was
over-represented
may
represent
host
predilection
mechanism
subspecies.
Together,
these
analyses
have
pathogenic
mechanisms
likely
important
zoonotic
infections.
IMPORTANCE
serious
humans,
including
skin
wound
pneumonia,
peritonitis,
meningitis,
bacteraemia.
Cats
are
vectors
pasteurellosis,
transmitting
via
bite
or
contact
animal
saliva.
underpin
pathogenesis
poorly
understood.
With
increasing
identification
antibiotic-resistant
strains,
understanding
vital
developing
treatments
control
strategies
to
combat
infection.
Here,
show
narrow
while
dogs,
common
harbor
wide
present
-specific
database,
quick
newly
sequenced
Antibiotics,
Год журнала:
2024,
Номер
13(2), С. 195 - 195
Опубликована: Фев. 19, 2024
Urinary
tract
infections
(UTIs)
caused
by
resistant
Klebsiella
pneumoniae
can
lead
to
severe
clinical
complications
and
even
death.
An
alternative
treatment
option
for
infected
patients
is
using
bacteriophages.
In
the
present
study,
we
isolated
phage
VB_KPM_KP1LMA
(KP1LMA)
from
sewage
water
a
K.
strain
as
host.
Whole-genome
analysis
indicated
that
genome
was
double-stranded
linear
176,096-bp
long
DNA
molecule
with
41.8%
GC
content
did
not
contain
virulence
or
antibiotic
resistance
genes.
The
inactivation
potential
of
KP1LMA
assessed
in
broth
at
an
MOI
1
10,
maximum
4.9
5.4
log
CFU/mL,
respectively,
observed
after
9
h.
efficacy
10
also
urine
evaluate
phage’s
performance
acidic
environment.
A
3.8
CFU/mL
results
suggest
could
potentially
control
UTI
this
pneumoniae,
indicating
same
procedure
be
used
UTIs
other
strains
if
new
specific
phages
are
isolated.
Although
has
narrow
host
range,
future,
efforts
made
expand
its
spectrum
activity
combine
others,
enabling
use
against
involved
UTIs.
Viruses,
the
majority
of
which
are
uncultivated,
among
most
abundant
biological
entities
on
Earth.
From
altering
microbial
physiology
to
driving
community
dynamics,
viruses
fundamental
members
microbiomes.
While
number
studies
leveraging
viral
metagenomics
(viromics)
for
studying
uncultivated
is
growing,
standards
viromics
research
lacking.
Viromics
can
utilize
computational
discovery
from
total
metagenomes
all
(hereafter
metagenomes)
or
use
physical
separation
virus-specific
fractions
viromes).
However,
differences
in
recovery
and
interpretation
viromes
obtained
same
samples
remain
understudied.
MetaCerberus
is
a
massively
parallel,
fast,
low
memory,
scalable
annotation
tool
for
inference
gene
function
across
genomes
to
metacommunities.
provides
an
elusive
HMM/HMMER-based
at
rapid
scale
with
memory.
It
offers
elucidation
major
public
databases,
including
KEGG
(KO),
COGs,
CAZy,
FOAM,
and
specific
databases
viruses,
VOGs
PHROGs,
from
single
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Май 15, 2024
is
a
bacterium
that
can
cause
food-borne
infections
and
responsible
for
the
most
common
gastrointestinal
illnesses.
The
emergence
of
multi-drug
resistant
(MDR)
strains
worldwide
major
threat,
representing
challenge
in
public
health.
To
reduce
its
incidence,
One
Health
approach
required,
development
new
biocontrol
protocols
will
help
prevent
or
eliminate
spread
Microbiology Spectrum,
Год журнала:
2024,
Номер
12(4)
Опубликована: Март 1, 2024
ABSTRACT
Pasteurella
multocida
is
an
upper
respiratory
tract
commensal
in
several
mammal
and
bird
species
but
can
also
cause
severe
disease
humans
production
animals
such
as
poultry,
cattle,
pigs.
In
this
study,
we
performed
whole-genome
sequencing
of
P.
isolates
recovered
from
a
range
human
infections,
the
mouths
cats,
wounds
on
dogs.
Together
with
publicly
available
genome
sequences,
phylogenetic
comparative
genomic
analyses.
While
cats
dogs
were
spread
across
tree,
infections
caused
almost
exclusively
by
subsp.
septica
strains.
Most
capsule
type
A
LPS
L1
L3;
however,
some
strains
lacked
biosynthesis
locus,
contained
novel
outer-core
distinct
eight
loci
that
currently
be
identified
using
multiplex
PCR.
addition,
isolated
mobile
genetic
elements.
We
compiled
curated
database
known
virulence
factor
antibiotic
resistance
genes
(PastyVRDB)
allowing
for
detailed
characterization
isolates.
The
majority
encoded
reduced
iron
receptors
only
one
filamentous
hemagglutinin
gene.
Finally,
gene-trait
analysis
putative
L-fucose
uptake
utilization
pathway
was
over-represented
may
represent
host
predilection
mechanism
subspecies.
Together,
these
analyses
have
pathogenic
mechanisms
likely
important
zoonotic
infections.
IMPORTANCE
serious
humans,
including
skin
wound
pneumonia,
peritonitis,
meningitis,
bacteraemia.
Cats
are
vectors
pasteurellosis,
transmitting
via
bite
or
contact
animal
saliva.
underpin
pathogenesis
poorly
understood.
With
increasing
identification
antibiotic-resistant
strains,
understanding
vital
developing
treatments
control
strategies
to
combat
infection.
Here,
show
narrow
while
dogs,
common
harbor
wide
present
-specific
database,
quick
newly
sequenced
