mSystems, Год журнала: 2024, Номер 9(3)

Опубликована: Фев. 20, 2024

ABSTRACT Bacterial plasmids play a major role in the spread of antibiotic resistance genes. However, their characterization via DNA sequencing suffers from low abundance plasmid those samples. Although sample preparation methods can enrich proportion before sequencing, these are expensive and laborious, they might introduce bias by enriching only for specific sequences. Nanopore adaptive sampling could overcome issues rejecting uninteresting molecules during process. In this study, we assess application enrichment low-abundant known bacterial isolates using two different tools. We show that significant be achieved even on expired flow cells. By applying sampling, also improve quality de novo assemblies reduce time. our experiments highlight with if target non-target sequences span similar regions. IMPORTANCE Antimicrobial causes millions deaths every year. Mobile genetic elements like key drivers dissemination antimicrobial This makes an important tool clinical microbiologists. Since often underrepresented samples, challenging laborious. To accelerate process, evaluate nanopore as silico method plasmids. Our results potential cost-efficient future research but indicate arise reference

Язык: Английский