Food Research International, Год журнала: 2024, Номер 197, С. 115171 - 115171
Опубликована: Сен. 30, 2024
Язык: Английский
ACS Nano, Год журнала: 2024, Номер unknown
Опубликована: Авг. 31, 2024
Metal–organic framework nanoparticles (MOF NPs) have received much attention for their potential use in nanopesticides. However, little is known about the health and environmental risks associated with these materials. In this study, toxicological responses of zebrafish exposed to five MOF NPs short long periods time were evaluated. The acute toxicity results showed that embryos adult was order Cu-MOF > ZIF-90 ZIF-8 Fe-MOF Zr-MOF. Histopathological analysis revealed ZIF-8, ZIF-90, caused liver swelling vacuolization zebrafish. cellular ultrastructure severely damaged mitochondrial structure intestinal epithelial cells cells. 16S rDNA sequencing data all significantly altered dominant microorganisms intestine. microbial markers inflammation, Proteobacteria (Aeromonas, Plesiomonas, Legionella), increased Fe-MOF, Zr-MOF, treatment groups. Metabolomics indicated levels inflammatory promoting factors (Leukotriene E4, 20-hydroxyeicosatetraenoic acid) arachidonic acid metabolism decreased, suppressing (8,9-epoxyeicosatrienoic increased. Metabolites related oxidative stress, such as glutamine, pyridoxamine, l-glutamic vitamin B6 other signaling pathways, reduced. Overall, suggest different had widely varying zebrafish, further should be paid real environment.
Язык: Английский
Процитировано
10
Cell Proliferation, Год журнала: 2023, Номер 57(1)
Опубликована: Июль 11, 2023
Abstract Cardiovascular diseases (CVDs) are the primary drivers of growing public health epidemic and leading cause premature mortality economic burden worldwide. With decades research, CVDs have been proven to be associated with dysregulation inflammatory response, macrophages playing imperative roles in influencing prognosis CVDs. Autophagy is a conserved pathway that maintains cellular functions. Emerging evidence has revealed an intrinsic connection between autophagy macrophage This review focuses on role underlying mechanisms autophagy‐mediated regulation plasticity polarization, inflammasome activation, cytokine secretion, metabolism, phagocytosis, number macrophages. In addition, shown connect heart cells. It attributed specific substrate degradation or signalling activation by autophagy‐related proteins. Referring latest reports, applications targeting discussed CVDs, such as atherosclerosis, myocardial infarction, failure, myocarditis. describes novel approach for future CVD therapies.
Язык: Английский
Процитировано
21
Frontiers in Physiology, Год журнала: 2024, Номер 15
Опубликована: Март 21, 2024
Cardiac fibrosis is a major and complex pathophysiological process that ultimately culminates in cardiac dysfunction heart failure. This phenomenon includes not only the replacement of damaged tissue by fibrotic scar produced activated fibroblasts/myofibroblasts but also spatiotemporal alteration structural, biochemical, biomechanical parameters ventricular wall, eliciting reactive remodeling process. Though mechanical stress, post-infarct homeostatic imbalances, neurohormonal activation are classically attributed to fibrosis, emerging evidence supports roles immune system modulation, inflammation, metabolic dysregulation initiation progression fibrogenesis has been reported. Adaptive changes, cell phenoconversions, shifts nonmyocyte population provide initial protection, persistent altered demand eventually contributes adverse heart. Altered energy metabolism, mitochondrial dysfunction, various cells, mediators, cross-talks between cells cardiomyocytes play crucial orchestrating transdifferentiation fibroblasts ensuing Manipulation plasticity, fibroblast–myofibroblast transition, modulation response may hold promise for favorably modulating following different cardiovascular pathological processes. Although immunologic perspectives being reported literature, they lack comprehensive sketch bridging these two arenas illustrating synchrony them. review aims overview intricate relationship pathways as well summarizes current understanding involvement immune–metabolic attempts identify some previously unaddressed questions require further investigation. Moreover, potential therapeutic strategies pharmacological interventions, including modulators, show preventing or attenuating restoring function will be discussed.
Язык: Английский
Процитировано
8
Ageing Research Reviews, Год журнала: 2024, Номер 104, С. 102629 - 102629
Опубликована: Дек. 5, 2024
Язык: Английский
Процитировано
6
Small Methods, Год журнала: 2024, Номер unknown
Опубликована: Июль 4, 2024
Abstract Lubricin, secreted primarily by chondrocytes, plays a critical role in maintaining the function of cartilage lubrication system. However, both external factors such as friction and internal like oxidative stress can disrupt this system, leading to osteoarthritis. Inspired lubricin, lubricating nanozyme, that is, Poly‐2‐acrylamide‐2‐methylpropanesulfonic acid sodium salt‐grafted aminofullerene, is developed restore system using an “In‐Out” strategy. The “Out” aspect involves reducing through combination hydration ball‐bearing lubrication. Simultaneously, “In” aims mitigate free radical, increasing autophagy, improving mitochondrial respiratory chain. This results reduced chondrocyte senescence increased lubricin production, enhancing natural ability cartilage. Transcriptome sequencing Western blot demonstrate it enhances functionality chain complexes I, III, V, thereby chondrocytes. In vitro vivo experiments show nanozymes reduce wear, improve senescence, damage, mitigating progression These findings provide novel insights into treating diseases associated with frictional osteoarthritis, set stage for future research development therapeutic interventions.
Язык: Английский
Процитировано
4
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Март 28, 2025
The intimate correlation between cardiovascular diseases and other organ pathologies, such as metabolic kidney diseases, underscores the intricate interactions among these organs. Understanding inter-organ communications is crucial for developing more precise drugs effective treatments systemic diseases. While animal models have traditionally been pivotal in studying interactions, human-induced pluripotent stem cells (hiPSCs) offer distinct advantages when constructing vitro models. Beyond conventional two-dimensional co-culture model, hiPSC-derived humanoid organoids emerged a substantial advancement, capable of replicating essential structural functional attributes internal organs vitro. This breakthrough has spurred development multilineage organoids, assembloids, organoids-on-a-chip technologies, which allow enhanced physiological relevance. These technologies shown great potential mimicking coordinated organogenesis, exploring disease pathogenesis, facilitating drug discovery. As central system, heart serves focal point an extensively studied network interactions. review focuses on advancements challenges organs, presenting comprehensive exploration this cutting-edge approach research.
Язык: Английский
Процитировано
0
Immunobiology, Год журнала: 2025, Номер 230(3), С. 152907 - 152907
Опубликована: Апрель 25, 2025
Macrophage autophagy plays a pivotal role in ischemia cardiomyopathy (ICM). However, the underlying mechanisms and macrophage autophagy-related biomarkers ICM have not been elucidated. Therefore, this study was designed to explore novel for ICM. The genes were downloaded from Human Autophagy Modulator intersected with differentially expressed (DEGs) of GSE46224 identified "limma" package R obtain DEGs. Immune infiltration analysis showed that macrophages dominant immune cells tissue. Then DEGs using weighted gene co-expression network (WGCNA). A total six hub obtained PPI network. All specific diagnostic significance AUCs higher than 0.7, as also validated external dataset GSE116250. RT-qPCR conducted detect mRNA expression levels vivo rat model. Single-cell RNA sequencing performed investigate profiles. Our explored their relative pathways ICM, provided gave new insight into progression mechanism
Язык: Английский
Процитировано
0
International Journal of Legal Medicine, Год журнала: 2025, Номер unknown
Опубликована: Апрель 2, 2025
Cardiovascular diseases are major causes of morbidity and death worldwide, most cardiac deaths related to ischemic injury the myocardium (myocardial infarction). As underlined in current clinical definition classification myocardial infarctions, not all injuries due ischemia: irreversible injury, ending necrosis, can be induced also by various other factors, such as infections, immune disorders, physical chemical agents, trauma. This is supported studies showing that elevated serum levels troponins, a measure damage, common finding non-ischemic types injury. Forensic pathologists confronted with autopsy findings suggestive should therefore realize both forms observed, only natural but non-natural (intoxications, asphyxia, traumatic iatrogenic deaths, others). Distinguishing these different underlying or circumstances critical, determine cause mechanism death, help investigate often challenging medico-legal scenarios. article reviews broad spectrum violent deaths. From this perspective we propose diagnostic approach forensic pathology context.
Язык: Английский
Процитировано
0
Aging, Год журнала: 2024, Номер 16(16), С. 11904 - 11916
Опубликована: Авг. 22, 2024
Background: Adriamycin (ADR) is a widely used chemotherapy drug in clinical practice and it causes toxicity the myocardium affecting its use. miR-432-5p miRNA primarily expressed myocardial cells has protective effect myocardium. We aim to explore of on ADR-caused impaired mitochondrial ATP metabolism endoplasmic reticulum stress (ERs). Method: The primary cardiomyocytes were obtained from neonatal mice ADR was added cells, meanwhile, model constructed through intravenous challenge, expression levels examined. Subsequently, introduced vitro vivo activity synthesis, autophagy, ER stress. bioinformatics analysis performed target miR-432-5p. Results: decreased cardiomyocytes. It also decreases production activates pathway by increasing LC3B, Beclin 1, cleaved caspase 3, induces cardiac toxicity. exogenous supplementation can reduce cardiotoxicity caused ADR, depends down-regulation RTN3 signaling ER. Conclusion: induce low miR-432-5p, activate ER, increase CHOP, RTN3,
Язык: Английский
Процитировано
2
Biosensors and Bioelectronics, Год журнала: 2024, Номер 270, С. 116954 - 116954
Опубликована: Ноя. 17, 2024
Язык: Английский
Процитировано
2