Unveiling distinct genetic features in multidrug-resistant Escherichia coli isolated from mammary tissue and gut of mastitis induced mice

Heliyon, Год журнала: 2024, Номер 10(5), С. e26723 - e26723

Опубликована: Фев. 21, 2024

is one of the major pathogens causing mastitis in lactating mammals. We hypothesized that

Язык: Английский

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Epidemiology of invasive Escherichia coli disease in adults in England, 2013–2017

Epidemiology and Infection, Год журнала: 2025, Номер 153

Опубликована: Янв. 1, 2025

Extraintestinal pathogenic

Язык: Английский

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Molecular mechanisms of re-emerging chloramphenicol susceptibility in extended-spectrum beta-lactamase-producing Enterobacterales

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Окт. 18, 2024

Infections with Enterobacterales (E) are increasingly difficult to treat due antimicrobial resistance. After ceftriaxone replaced chloramphenicol (CHL) as empiric therapy for suspected sepsis in Malawi 2004, extended-spectrum beta-lactamase (ESBL)-E rapidly emerged. Concurrently, resistance CHL Escherichia coli and Klebsiella spp. decreased, raising the possibility of re-introduction. However, many phenotypically susceptible isolates still carry acetyltransferase (cat) genes. To understand molecular mechanisms stability this re-emerging susceptibility we use a combination genomics, phenotypic assays, experimental evolution, functional assays CAT activity. Here, show that 840 Malawian E. isolates, 31% have discordant genotype-phenotype, select subset 42 in-depth analysis. Stable degradation cat genes by insertion sequences leads re-emergence susceptibility. Our study suggests could be reintroduced reserve agent critically ill patients ESBL-E infections similar settings highlights ongoing challenges inferring from sequence data.

Язык: Английский

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Phytochemicals Controlling Enterohemorrhagic Escherichia coli (EHEC) Virulence—Current Knowledge of Their Mechanisms of Action

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 381 - 381

Опубликована: Янв. 4, 2025

Enterohemorrhagic Escherichia coli (EHEC) is a common pathotype of E. that causes numerous outbreaks foodborne illnesses. EHEC zoonotic pathogen transmitted from animals to humans. Ruminants, particularly cattle, are considered important reservoirs for virulent strains. Humans can become infected with through the consumption contaminated food and water or direct contact O157:H7 one most commonly reported illnesses in developed countries. The formation attaching effacing (A/E) lesions on intestinal epithelium, combined Shiga toxin production, hallmark infection lead lethal hemolytic–uremic syndrome (HUS). For phage-dependent regulation antibiotic treatment contraindicated, as it may exacerbate limiting therapeutic options supportive care. In response this challenge growing threat resistance, phytochemicals have emerged promising antivirulence agents. These plant-derived compounds target bacterial virulence mechanisms without promoting resistance. Therefore, aim study summarize recent knowledge use targeting EHEC. We focused molecular basis their action, principal determinants

Язык: Английский

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Economic and Disease Burden Associated with Invasive Escherichia coli Disease in the United States

Infectious Diseases and Therapy, Год журнала: 2025, Номер unknown

Опубликована: Фев. 8, 2025

Invasive Escherichia coli disease (IED) incidence has increased over recent years among aging populations and rising antimicrobial resistance. Here, we report on a comparative, cross-sectional, retrospective analysis of US patients with IED to quantify IED-related healthcare resource utilization (HCRU), costs, impact health-related quality life (HRQoL). This study included Kaiser Permanente Northwest (KPNW) members aged ≥ 60 enrolled between July 2019 January 2020. Patients were divided into three groups: Group 1 had experienced episode (≤ 3 weeks before enrollment); 2 former (13–18 months was at risk no prior history IED. Data collected from electronic hospital records, patient survey, the EuroQol 5-Dimension 5-Level (EQ-5D-5L) questionnaire. Mean costs adjusted according individual follow-up. Patient characteristics generally consistent across Groups (n = 289), 319), 340). Inpatient hospitalization observed in 84%, 44%, 15% 1, 3, respectively. direct per (per 30-day follow-up) $17,168, $2530, $1094 2, total year following an (Group 2) $35,034 vs. $16,163 at-risk 3. HRQoL poor for IED, mean EQ-5D-5L utility index value 0.25 worst day illness. During 12-month follow-up period, rehospitalization rates number antibiotic prescriptions ~ threefold higher who recovered those risk. These data demonstrate substantial short- long-term impacts HCRU, HRQoL. Additional research is needed further novel prevention strategies.

Язык: Английский

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Epidemiology, Clinical Features, and Antimicrobial Resistance of InvasiveEscherichia ColiDisease in Patients Admitted in Tertiary Care Hospitals

Open Forum Infectious Diseases, Год журнала: 2023, Номер 10(2)

Опубликована: Янв. 27, 2023

Invasive Escherichia coli disease (IED), including bloodstream infection, sepsis, and septic shock, can lead to high hospitalization mortality rates. This multinational study describes the clinical profile of patients with IED in tertiary care hospitals. We applied criteria systemic inflammatory response syndrome (SIRS), or shock hospitalized culture-confirmed E from urine a presumed sterile site. assessed proposed case definition against physician diagnoses. Most (N = 902) were adults aged ≥60 years (76.5%); 51.9%, 25.1%, 23.0% cases community-acquired (CA), hospital-acquired (HA), healthcare-associated (HCA), respectively. The urinary tract was most common source infection (52.3%). Systemic syndrome, identified 77.4%, 65.3%, 14.1% patients, Patients >60 more likely exhibit organ dysfunction than those ≤60 years; this trend not observed for SIRS. case-fatality rate (CFR) 20.0% (60-75 years, 21.5%; ≥75 22.2%), an increase across acquisition settings (HA, 28.3%; HCA, 21.7%; CA, 15.2%). Noticeably, 77.8% initiated antibiotic use on day culture sample collection. A total 65.6% 40.8% isolates resistant ≥1 agent ≥2 drug class(es). 96.1% agreement seen between physician's diagnoses IED. contributes valuable, real-world data about severity. An accepted could promote timely accurate diagnosis inform development novel preventative strategies.

Язык: Английский

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Genome-Based Characterization of Hybrid Shiga Toxin-Producing and Enterotoxigenic Escherichia coli (STEC/ETEC) Strains Isolated in South Korea, 2016–2020

Microorganisms, Год журнала: 2023, Номер 11(5), С. 1285 - 1285

Опубликована: Май 15, 2023

The global emergence of hybrid diarrheagenic E. coli strains incorporating genetic markers from different pathotypes is a public health concern. Hybrids Shiga toxin-producing and enterotoxigenic (STEC/ETEC) are associated with diarrhea hemolytic uremic syndrome (HUS) in humans. In this study, we identified characterized STEC/ETEC isolated livestock feces (cattle pigs) animal food sources (beef, pork, meat patties) South Korea between 2016 2020. were positive for genes STEC ETEC, such as stx (encodes toxins, Stxs) est heat-stable enterotoxins, ST), respectively. belong to diverse serogroups (O100, O168, O8, O155, O2, O141, O148, O174) sequence types (ST446, ST1021, ST21, ST74, ST785, ST670, ST1780, ST1782, ST10, ST726). Genome-wide phylogenetic analysis revealed that these hybrids closely related certain ETEC strains, implying the potential acquisition Stx-phage and/or virulence during hybrids. Particularly, source foods mostly exhibited close relatedness strains. These findings allow further exploration pathogenicity may serve data future comparative studies evolutionary biology.

Язык: Английский

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Safety, Reactogenicity, Immunogenicity, and Dose Selection of 10-Valent Extraintestinal Pathogenic Escherichia coli Bioconjugate Vaccine (VAC52416) in Adults Aged 60–85 Years in a Randomized, Multicenter, Interventional, First-in-Human, Phase 1/2a Study

Open Forum Infectious Diseases, Год журнала: 2023, Номер 10(8)

Опубликована: Авг. 1, 2023

ExPEC10V is a bioconjugate vaccine containing O-antigen polysaccharides of 10 extraintestinal pathogenic Escherichia coli (ExPEC) serotypes. This phase 1/2a study (NCT03819049) assessed the safety, reactogenicity, and immunogenicity (VAC52416) to prevent invasive E disease in elderly adults.The observer-blind, active-controlled design included 28-day screening, vaccination, 181-day follow-up, 1-year follow-up. Participants (60-85 years age) were randomized low dose (antigen range, 4-8 µg), medium (4-16 or high (8-16 µg); 4-valent ExPEC (ExPEC4V); 13-valent pneumococcal conjugate (PCV13). The incidence adverse events (AEs; solicited, day 15; unsolicited, 30; serious AEs, 181) (electrochemiluminescent-based assay [ECL] multiplex opsonophagocytic [MOPA]) assessed. Optimal was determined from safety data through 30 an selection algorithm based on 15 ECL MOPA results.A total 416 participants (median age, 64.0 years; 54.8% female). incidences solicited local systemic AEs were, respectively, 44.2% 39.4% for low-dose, 52.9% 46.1% medium-dose, 57.7% 45.2% high-dose ExPEC10V, 74.1% 48.1% PCV13. Five not related, reported. revealed robust antibody response year 1. Opsonophagocytic killing activity detected against all but serotype O8; this lack O8 linked sensitivity. Based totality data, considered optimal.ExPEC10V well tolerated immunogenic adults O8.

Язык: Английский

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Genomic profiling of extended-spectrum β-lactamase-producing Escherichia coli from Pets in the United Arab Emirates: Unveiling colistin resistance mediated by mcr-1.1 and its probable transmission from chicken meat – A One Health perspective

Journal of Infection and Public Health, Год журнала: 2023, Номер 16, С. 163 - 171

Опубликована: Ноя. 3, 2023

The United Arab Emirates (UAE) has witnessed rapid urbanization and a surge in pet ownership, sparking concerns about the possible transfer of antimicrobial resistance (AMR) from pets to humans environment. This study delves into whole-genome sequencing analysis ESBL-producing E. coli strains healthy cats dogs UAE, which exhibit multidrug (MDR). Additionally, it provides genomic exploration mobile colistin gene mcr-1.1, marking first instance its detection Middle Eastern pets.We investigate 17 UAE using WGS bioinformatics identify genes encoding virulence factors, assign diverse typing schemes isolates, scrutinize presence AMR genes. Furthermore, we characterized plasmid contigs housing mcr-1.1 conducted phylogenomic evaluate their relatedness previously identified isolates.Our unveiled variety factor-encoding within with fimH emerging as most prevalent. Regarding β-lactamase genes, blaCTX group 1 family predominated, CTX-M-15 found 52.9% (9/17) followed by CTX-M-55 29.4% (5/17). These isolates were categorized multiple sequence types (STs), epidemic ST131 being frequent. gene, linked resistance, was confirmed two isolates. belonged ST1011 displayed distinct profiles Phylogenomic revealed close connections between those chicken meat UAE.Our underscores MDR pets. identification mcr-1.1-carrying warrants urgency comprehensive surveillance highlights role companion animals epidemiology. findings underscore significance adopting One Health approach mitigate transmission risks effectively.

Язык: Английский

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Clinical burden of invasive Escherichia coli disease among older adult patients treated in hospitals in the United States

BMC Infectious Diseases, Год журнала: 2023, Номер 23(1)

Опубликована: Авг. 22, 2023

Invasive extraintestinal pathogenic Escherichia coli disease (IED) can lead to severe outcomes, particularly among older adults. However, the clinical burden of IED in U.S. has not been well characterized.IED encounters patients ≥ 60 years old were identified using PINC AI™ Healthcare Database (10/01/2015-03/31/2020) by either a positive E. culture blood or another normally sterile body site and 1 sign systemic inflammatory response syndrome signs sepsis, urine with urinary tract infection sepsis. Medical resource utilization, isolate characteristics descriptively reported during first encounter following year (observation period).Overall, 19,773 included (mean age: 76.8 years; 67.4% female; 78.5% sepsis). Most involved community-onset (94.3%) required hospitalization (96.5%; mean duration: 6.9 days), 32.4% being admitted intensive care unit 3.7 days). isolates resistant antibiotic category (61.7%) 34.4% 3 categories. Following their encounter, 34.8% transferred skilled nursing/intermediate facility, whereas 6.8% had died. During observation period, 36.8% rehospitalized, 2.4% recurrence, in-hospital death increased 10.9%.IED is associated substantial at considerable long-term consequences. Findings demonstrate need for awareness highlight potential benefits prevention.

Язык: Английский

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