The Future of Pathogen Detection in Aquaculture: Miniature Labs, Field‐Compatible Assays, environmental DNA and RNA, CRISPR and Metatranscriptomics DOI Creative Commons
Jessica Rieder,

Anastasiia Berezenko,

Alexandra Meziti

и другие.

Aquaculture Fish and Fisheries, Год журнала: 2025, Номер 5(3)

Опубликована: Май 5, 2025

ABSTRACT Inland recirculating aquaculture is a thriving food industry providing sustainable and locally sourced high‐quality protein. However, its expansion accompanied by emerging challenges regarding the spread of pathogens diseases. Detection management in remain underdeveloped compared to other animal farming sectors due vast diversity species involved, non‐domesticated limited knowledge pathogens, host responses disease mechanisms. Furthermore, systems are heavily dependent on beneficial bacterial communities for waste product removal water quality maintenance, with opportunistic constituting an inherent component these microbial communities. To enhance potential inland as source protein, it imperative adopt advanced tools pathogen detection monitoring assessing overall health ecosystem. This paper presents overview promising current molecular technological advancements that offer solutions system aquaculture. We focus recently developed point‐of‐care on‐site methods using miniaturized laboratory equipment robust workflows operate independently cold chain logistics. explore methodologies environment rather than through fish assessments. Lastly, we discuss techniques from scientific disciplines aquaculture, including CRISPR‐Cas protocols implementation ‘omics’ approaches comprehensive characterization states. These demonstrate considerable surveillance and, subsequently, early dynamic field. Through better understanding available options, managers scientists can collaborate optimize systems. aims facilitate communication between managers, equipping their facilities.

Язык: Английский

Diversity of sulfur cycling halophiles within the Salton Sea, California’s largest lake DOI Creative Commons

Linton Freund,

Caroline Hung,

Talyssa M. Topacio

и другие.

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 6, 2025

Microorganisms are the biotic foundation for nutrient cycling across ecosystems, and their assembly is often based on availability of environment. Though previous research has explored seasonal lake turnover geochemical within Salton Sea, California's largest lake, microbial community this declining ecosystem been largely overlooked. We collected seawater from a single location Sea at 0 m, 3 4 5 7 9 10 10.5 m depths in August 2021, December April 2022. observed that water column microbiome significantly varied by season (R2 = 0.59, P 0.003). Temperature 0.27, 0.004), dissolved organic matter 0.13, oxygen 0.089, 0.004) were significant drivers changes composition. In addition, several halophilic mixotrophs other extremotolerant bacteria consistently identified samples time points, though relative abundances fluctuated season. found while sulfur genes present all metagenomes, coverages pathway throughout column. Sulfur oxidation incomplete pathways conserved seasons. Our work demonstrates Seawater capacity to metabolize species utilize multiple trophic strategies, such as alternating between chemorganotrophy chemolithoautrophy, survive harsh, fluctuating Together, these results suggest integral ever-changing thus contributes dynamics Sea. Further required understand how environmental implicated relationship Sea's cycle, dust proliferation, respiratory distress experienced local population.

Язык: Английский

Процитировано

1

Characterising Measures of Functional Redundancy in, Microbiome Communities via Relative Entropy DOI Creative Commons
Daniel Fässler, Almut Heinken, Johannes Hertel

и другие.

Computational and Structural Biotechnology Journal, Год журнала: 2025, Номер 27, С. 1482 - 1497

Опубликована: Янв. 1, 2025

Functional redundancy has been hypothesised to be at the core of well-evidenced relation between high ecological microbiome diversity and human health. Here, we conceptualise operationalise functional on a single-trait level for functionally annotated microbial communities, utilising an information-theoretic approach based relative entropy that also allows quantification interdependency across species. Via constraint-based community modelling public faecal metagenomic dataset, demonstrate strength species is dependent specific attributes function under consideration such as rarity occurring interdependencies. Moreover, by integrating metabolome data, highlight measures have correlates in host's metabolome. We further microbiomes sampled from colorectal cancer patients display higher levels species-species interdependencies than those healthy controls. By analysing models inflammatory bowel disease (IBD) study, show although decreased IBD subjects, increased certain metabolites, notably hydrogen sulphide. This finding highlights their potential provide valuable insights beyond diversity. formalise concept communities its usefulness real providing foundation deeper understanding how shapes capacities microbiome.

Язык: Английский

Процитировано

0

The Impact of Antibiotic Therapy on Intestinal Microbiota: Dysbiosis, Antibiotic Resistance, and Restoration Strategies DOI Creative Commons

Gaia Cusumano,

Giancarlo Angeles Flores, Roberto Venanzoni

и другие.

Antibiotics, Год журнала: 2025, Номер 14(4), С. 371 - 371

Опубликована: Апрель 3, 2025

The human gut microbiota—an intricate and dynamic ecosystem—plays a pivotal role in metabolic regulation, immune modulation, the maintenance of intestinal barrier integrity. Although antibiotic therapy is indispensable for managing bacterial infections, it profoundly disrupts microbial communities. Such dysbiosis typified by diminished diversity shifts community structure, especially among beneficial genera (e.g., Bifidobacterium Eubacterium), fosters antibiotic-resistant strains horizontal transfer resistance genes. These alterations compromise colonization resistance, increase permeability, amplify susceptibility to opportunistic pathogens like Clostridioides difficile. Beyond gastrointestinal disorders, emerging evidence associates with systemic conditions, including chronic inflammation, syndrome, neurodegenerative diseases, underscoring relevance microbiota–gut–brain axis. recovery pre-existing communities post-antibiotic highly variable, influenced drug spectrum, dosage, treatment duration. Innovative interventions—such as fecal microbiota transplantation (FMT), probiotics, synbiotics, precision microbiome therapeutics—have shown promise counteracting mitigating its adverse effects. therapies align closely stewardship programs aimed at minimizing unnecessary use preserve curtail spread multidrug-resistant organisms. This review emphasizes pressing need microbiota-centered strategies optimize administration, promote long-term health resilience, alleviate disease burden associated antibiotic-induced dysbiosis.

Язык: Английский

Процитировано

0

Biochar and PGPR Influence Rhizosphere Bacteria and Synergistically Regulate Tomato Seedling Biomass Under Greenhouse Conditions DOI
Zhiqiang Yan,

Yuyuan Xing,

Changli Ma

и другие.

Journal of soil science and plant nutrition, Год журнала: 2025, Номер unknown

Опубликована: Апрель 25, 2025

Язык: Английский

Процитировано

0

The Future of Pathogen Detection in Aquaculture: Miniature Labs, Field‐Compatible Assays, environmental DNA and RNA, CRISPR and Metatranscriptomics DOI Creative Commons
Jessica Rieder,

Anastasiia Berezenko,

Alexandra Meziti

и другие.

Aquaculture Fish and Fisheries, Год журнала: 2025, Номер 5(3)

Опубликована: Май 5, 2025

ABSTRACT Inland recirculating aquaculture is a thriving food industry providing sustainable and locally sourced high‐quality protein. However, its expansion accompanied by emerging challenges regarding the spread of pathogens diseases. Detection management in remain underdeveloped compared to other animal farming sectors due vast diversity species involved, non‐domesticated limited knowledge pathogens, host responses disease mechanisms. Furthermore, systems are heavily dependent on beneficial bacterial communities for waste product removal water quality maintenance, with opportunistic constituting an inherent component these microbial communities. To enhance potential inland as source protein, it imperative adopt advanced tools pathogen detection monitoring assessing overall health ecosystem. This paper presents overview promising current molecular technological advancements that offer solutions system aquaculture. We focus recently developed point‐of‐care on‐site methods using miniaturized laboratory equipment robust workflows operate independently cold chain logistics. explore methodologies environment rather than through fish assessments. Lastly, we discuss techniques from scientific disciplines aquaculture, including CRISPR‐Cas protocols implementation ‘omics’ approaches comprehensive characterization states. These demonstrate considerable surveillance and, subsequently, early dynamic field. Through better understanding available options, managers scientists can collaborate optimize systems. aims facilitate communication between managers, equipping their facilities.

Язык: Английский

Процитировано

0