microRNA-1 Regulates Metabolic Flexibility in Skeletal Muscle via Pyruvate Metabolism

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 10, 2024

Abstract MicroRNA-1 (miR-1) is the most abundant miRNA in adult skeletal muscle. To determine function of miR-1 muscle, we generated an inducible, muscle-specific knockout (KO) mouse. Integration RNA-sequencing (RNA-seq) data from KO muscle with Argonaute 2 enhanced crosslinking and immunoprecipitation sequencing (AGO2 eCLIP-seq) human identified target genes involved glycolysis pyruvate metabolism. The loss induced cancer-like metabolic reprogramming, as shown by higher kinase isozyme M2 (PKM2) protein levels, which promoted glycolysis. Comprehensive bioenergetic phenotyping combined proteomics metabolomics further demonstrated that inflexibility a result oxidation resistance. While genetic reduced endurance exercise performance mice C. elegans, physiological down-regulation expression response to hypertrophic stimulus both humans causes similar reprogramming supports cell growth. Taken together, these identify novel post-translational mechanism metabolism regulation mediated miR-1.

Язык: Английский

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Epigenome–proteome integration in aged skeletal muscle after exercise training

The Journal of Physiology, Год журнала: 2024, Номер 603(1), С. 29 - 31

Опубликована: Сен. 19, 2024

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The risks and rewards of the synergist ablation model in skeletal muscle biology research

AJP Cell Physiology, Год журнала: 2024, Номер 327(3), С. C599 - C600

Опубликована: Июль 29, 2024

Язык: Английский

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