A Nematode Effector, MiMSP8, Targets U2AF, a Subunit of a Splicing Factor Involved in Host pre‐mRNA Splicing, Thereby Promoting Parasitism DOI
Cong Chen, Wenjun Hu, Chen Chen

и другие.

Plant Cell & Environment, Год журнала: 2025, Номер unknown

Опубликована: Июнь 3, 2025

ABSTRACT Root‐knot nematodes secrete effectors into plant cells to facilitate parasitism. A candidate effector, MiMSP8, of Meloidogyne incognita has been shown localise within the nucleus when transiently expressed in Nicotiana benthamiana leaf cells, but its role is still unknown. We demonstrate that MiMSP8 protein dorsal gland M. juveniles and it can also be detected giant induced by nematodes. Silencing impairs nematode parasitism while overexpression increases susceptibility infection. interacts with SlU2AF35, small subunit U2 snRNP auxiliary factor (U2AF) tomato. This interaction competes SlU2AF65 for binding thereby disrupting formation heterodimers key splicing U2AF. Overexpression tomato hairy roots leads genome‐wide alternative changes involved multiple biological processes. interferes between SlU2AF35 pre‐mRNAs a subset genes. results abnormal gene plants their sensitivity Collectively, our findings reveal deploys nuclear localised effector target component spliceosome disrupt pre‐mRNA promote

Язык: Английский

A Nematode Effector, MiMSP8, Targets U2AF, a Subunit of a Splicing Factor Involved in Host pre‐mRNA Splicing, Thereby Promoting Parasitism DOI
Cong Chen, Wenjun Hu, Chen Chen

и другие.

Plant Cell & Environment, Год журнала: 2025, Номер unknown

Опубликована: Июнь 3, 2025

ABSTRACT Root‐knot nematodes secrete effectors into plant cells to facilitate parasitism. A candidate effector, MiMSP8, of Meloidogyne incognita has been shown localise within the nucleus when transiently expressed in Nicotiana benthamiana leaf cells, but its role is still unknown. We demonstrate that MiMSP8 protein dorsal gland M. juveniles and it can also be detected giant induced by nematodes. Silencing impairs nematode parasitism while overexpression increases susceptibility infection. interacts with SlU2AF35, small subunit U2 snRNP auxiliary factor (U2AF) tomato. This interaction competes SlU2AF65 for binding thereby disrupting formation heterodimers key splicing U2AF. Overexpression tomato hairy roots leads genome‐wide alternative changes involved multiple biological processes. interferes between SlU2AF35 pre‐mRNAs a subset genes. results abnormal gene plants their sensitivity Collectively, our findings reveal deploys nuclear localised effector target component spliceosome disrupt pre‐mRNA promote

Язык: Английский

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