Genomic diversity of phages infecting the globally widespread genus Sulfurimonas DOI Creative Commons
Xiaofeng Li, Ruolin Cheng, Chuan‐Xi Zhang

и другие.

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Ноя. 2, 2024

The widespread bacterial genus Sulfurimonas is metabolically versatile and occupies a key ecological niche in different habitats, but its interaction with bacteriophages remains unexplored. Here we systematically investigated the genetic diversity, taxonomy patterns of Sulfurimonas-associated phages based on sequenced microbial genomes metagenomes. High-confidence phage contigs related to were retrieved from various ecosystems, clustered into 61 viral operational taxonomic units across three realms, including Duplodnaviria, Monodnaviria Varidnaviria. Head-tail Caudoviricetes assigned 19 genus-level clusters, majority which distantly known viruses. Notably, diverse double jelly-roll viruses inoviruses also linked Sulfurimonas, representing two commonly overlooked groups. Historical current infections revealed, implying impact evolution host adaptive immunity. Additionally, carrying auxiliary metabolic genes might benefit hosts by compensating or augmenting sulfur metabolism. This study highlights diversity novelty divergent tailless lineages, providing basis for further investigation phage-host interactions within this genus.

Язык: Английский

The response mechanisms of kelp Macrocystis pyrifera holobiont to elevated temperature and CO2 concentration DOI

Tianle Xi,

Yajing Liu, Xiaowen Zhang

и другие.

Journal of Applied Phycology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 13, 2025

Язык: Английский

Процитировано

0

Salinity as a key factor affects viral structure, function, and life strategies in lakes from arid and semi-arid regions DOI

Haojun Lei,

Nicolas Zhou, Jinhong Zhang

и другие.

Journal of Hazardous Materials, Год журнала: 2025, Номер unknown, С. 138075 - 138075

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Candidate Phyla Radiation (CPR) bacteria from hyperalkaline ecosystems provide novel insight into their symbiotic lifestyle and ecological implications DOI Creative Commons
Yu He,

Shiyan Zhuo,

Meng Li

и другие.

Microbiome, Год журнала: 2025, Номер 13(1)

Опубликована: Апрель 7, 2025

Abstract Background Candidate Phyla Radiation (CPR) represents a unique superphylum characterized by ultra-small cell size and symbiotic lifestyle. Although CPR bacteria have been identified in varied environments, their broader distribution, associations with hosts, ecological roles remain largely unexplored. To address these knowledge gaps, serpentinite-like environment was selected as simplified model system to investigate the communities hyperalkaline environments association hosts extreme conditions. Additionally, enzymatic activity, global evolution of CPR-derived genes encoding essential metabolites (e.g., folate or vitamin B 9 ) were analyzed assessed. Results In highly alkaline ecosystem (pH = 10.9–12.4), metagenomic analyses water sediment samples revealed that constituted 1.93–34.8% microbial communities. Metabolic reconstruction 12 high-quality metagenome-assembled genomes (MAGs) affiliated novel taxa from orders UBA6257, UBA9973, Paceibacterales suggests lack complete biosynthetic pathways for amino acids, lipids, nucleotides. Notably, commonly harbored associated cofactor biosynthesis metabolism, including dihydrofolate reductase ( folA ), serine hydroxymethyltransferase glyA methylenetetrahydrofolate folD ). two presumed auxotrophic incapable forming tetrahydrofolate (THF) due absence , potential some harboring genes. The functionality experimentally verified heterologous expression -deletion mutant Escherichia coli MG1655 Δ . Further assessment available n 4,581) proteins synthesis bioactive derivatives and/or genes) present 90.8% examined. It widespread metabolic complementarity between hosts. Conclusions This finding deepens our understanding mechanisms CPR-host symbiosis, providing insight into cofactor-dependent mutualistic interactions. Our observations suggest may contribute organisms indirectly influence biogeochemical processes.

Язык: Английский

Процитировано

0

Engineering microbiomes to enhance macroalgal health, biomass yield, and carbon sequestration DOI Creative Commons
Shailesh Nair, Zenghu Zhang,

Xiaojie Wang

и другие.

Green Carbon, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

1

Discovery of new and variant Potyviruses in Asparagales plants from a Dutch urban botanic garden DOI Creative Commons
Rob J. Dekker, Han Rauwerda, Wim C. de Leeuw

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 10, 2024

Abstract Although viruses play an important role in human health and plant health, most are still undetected. One of the reasons is that they may evolve distinct natural habitats not yet extensively sampled for virus analysis. An example such a habitat urban botanic gardens. We analyzed 25 Asparagales plants with mild to severe disease phenotype from Dutch garden presence known unknown types variants by smallRNA-seq RNA-seq. found all samples evidence (past) Potyviridae presence, mostly Ornithogalum (OV) mosaic (OrMV), as well new Iris variant (IMMV) species Potyvirus. Also, Betaflexivirus, Polerovirus Phenuivirus were detected. Most harbored multiple viruses, 18 out showed three seven 12 13 present four 11 samples. In this study, we describe characteristics newly discovered Potyvirus identify several potyviruses. place these findings context viruses. However, unable link potyviruses any specific phenotype.

Язык: Английский

Процитировано

0

Genomic diversity of phages infecting the globally widespread genus Sulfurimonas DOI Creative Commons
Xiaofeng Li, Ruolin Cheng, Chuan‐Xi Zhang

и другие.

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Ноя. 2, 2024

The widespread bacterial genus Sulfurimonas is metabolically versatile and occupies a key ecological niche in different habitats, but its interaction with bacteriophages remains unexplored. Here we systematically investigated the genetic diversity, taxonomy patterns of Sulfurimonas-associated phages based on sequenced microbial genomes metagenomes. High-confidence phage contigs related to were retrieved from various ecosystems, clustered into 61 viral operational taxonomic units across three realms, including Duplodnaviria, Monodnaviria Varidnaviria. Head-tail Caudoviricetes assigned 19 genus-level clusters, majority which distantly known viruses. Notably, diverse double jelly-roll viruses inoviruses also linked Sulfurimonas, representing two commonly overlooked groups. Historical current infections revealed, implying impact evolution host adaptive immunity. Additionally, carrying auxiliary metabolic genes might benefit hosts by compensating or augmenting sulfur metabolism. This study highlights diversity novelty divergent tailless lineages, providing basis for further investigation phage-host interactions within this genus.

Язык: Английский

Процитировано

0