Exploratory miRNA profiling from serum and bone tissue of mice with T1D-induced bone loss DOI Creative Commons

Souad Daamouch,

Andreas B. Diendorfer, Matthias Hackl

и другие.

Frontiers in Endocrinology, Год журнала: 2024, Номер 15

Опубликована: Дек. 24, 2024

Type 1 diabetes (T1D) represents a significant health burden worldwide, with associated complications including bone fragility. Current clinical methods and biomarkers for assessing predicting fracture risk in T1D are limited lack accuracy. MicroRNAs (miRNAs) have emerged as potential T1D-induced loss, although comprehensive profiling studies lacking. Previous investigations indicated link between dysregulated miRNA expression levels impaired T1D. Therefore, this study, we explored differential serum tissue of mice loss using Next Generation Sequencing (NGS). was induced streptozotocin male wild-type mice. Serum tissues were analyzed at 14 weeks age, following the prior characterization mouse model. MiRNA conducted two-independent NGS analyses validated through quantitative RT-PCR. identified miRNAs compared to controls. The first analysis revealed 24 differentially expressed 13 tissue. Especially, miR-136-3p consistently downregulated both However, second presented distinct set miRNAs, miR-206-3p overlapping but exhibiting patterns. Surprisingly, miR-144-5p, miR-19a-3p, miR-21a-5p displayed contrasting regulatory patterns qPCR analyses. Finally, gene network associations pathways involved physiology, TGF-beta, PI3-Akt signaling, osteoclast differentiation humans. In conclusion, our study offers initial insights into also highlights consistency results obtained from sequencing different cohorts. Thus, further investigation is needed better understand complexities before they can be established reproducible

Язык: Английский

microRNAs in Type 1 Diabetes: Roles, Pathological Mechanisms, and Therapeutic Potential DOI Open Access
H. Cho, Se Eun Ha, Rajan Singh

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3301 - 3301

Опубликована: Апрель 2, 2025

Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by the progressive destruction of pancreatic β-cells, leading to insulin deficiency. The primary drivers β-cell in T1D involve autoimmune-mediated processes that trigger inflammation and ultimately loss. Regulatory microRNAs (miRNAs) play crucial role modulating these regulating gene expression through post-transcriptional suppression target mRNAs. Dysregulated miRNAs have been implicated pathogenesis, serving as both potential diagnostic biomarkers therapeutic targets. This review explores T1D, highlighting their involvement mechanisms across rodent models human patients. While current antidiabetic therapies manage symptoms, they do not prevent destruction, leaving patients reliant on lifelong therapy. By summarizing key miRNA profiles diabetic animal patients, this miRNA-based restore function halt or slow progression disease.

Язык: Английский

Процитировано

0
Exploratory miRNA profiling from serum and bone tissue of mice with T1D-induced bone loss DOI Creative Commons

Souad Daamouch,

Andreas B. Diendorfer, Matthias Hackl

и другие.

Frontiers in Endocrinology, Год журнала: 2024, Номер 15

Опубликована: Дек. 24, 2024

Type 1 diabetes (T1D) represents a significant health burden worldwide, with associated complications including bone fragility. Current clinical methods and biomarkers for assessing predicting fracture risk in T1D are limited lack accuracy. MicroRNAs (miRNAs) have emerged as potential T1D-induced loss, although comprehensive profiling studies lacking. Previous investigations indicated link between dysregulated miRNA expression levels impaired T1D. Therefore, this study, we explored differential serum tissue of mice loss using Next Generation Sequencing (NGS). was induced streptozotocin male wild-type mice. Serum tissues were analyzed at 14 weeks age, following the prior characterization mouse model. MiRNA conducted two-independent NGS analyses validated through quantitative RT-PCR. identified miRNAs compared to controls. The first analysis revealed 24 differentially expressed 13 tissue. Especially, miR-136-3p consistently downregulated both However, second presented distinct set miRNAs, miR-206-3p overlapping but exhibiting patterns. Surprisingly, miR-144-5p, miR-19a-3p, miR-21a-5p displayed contrasting regulatory patterns qPCR analyses. Finally, gene network associations pathways involved physiology, TGF-beta, PI3-Akt signaling, osteoclast differentiation humans. In conclusion, our study offers initial insights into also highlights consistency results obtained from sequencing different cohorts. Thus, further investigation is needed better understand complexities before they can be established reproducible

Язык: Английский

Процитировано

0