Cloning, Expression, and Odorant Binding Characteristics of the Chemosensory Protein 1 in Asecodes hispinarum, a Parasitoid of the Coconut Leaf Beetle (Brontispa longissima) DOI
Keming Li, Fuping Lu,

Huazhou Wu

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Май 7, 2025

Abstract Asecodes hispinarum (Hymenoptera: Eulophidae) is an endoparasitic wasp and efficient biological control agent of the coconut leaf beetle, Brontispa longissima, a significant pest more than 20 palm species. Due to its effectiveness against B. A. extensively utilized in management palms such as coconut, betel nut, oil palm. Olfaction plays crucial role insect behaviors finding food, selecting oviposition sites, locating mates. However, molecular mechanisms underlying olfactory recognition have not been reported. In previous study, we identified several olfaction-related genes through transcriptome sequencing bioinformatics analysis. current chemosensory protein gene 1 (AhisCSP1) was cloned, real-time PCR employed assess expression levels AhisCSP1 across different developmental stages adult tissues hispinarum. Additionally, odorant binding characteristics were examined using vitro technology fluorescence competitive assays. The results indicated that expressed at all stages, with higher stage compared other stages. Furthermore, specific antennae, no detected head, thorax, abdomen, legs, or wings. A assay revealed can effectively bound volatiles L-α-terpineol, benzyl alcohol, 3-phenylpropionic acid pentyl ester 2-ethyl-1-hexanol, constants 21.26, 25.42, 27.96, 39.72 µmol/L, respectively. Among four longissima larvae, demonstrated strong capacity isohexadecane, constant 19.76 medium pentadecane, 34.28 weak butyl benzoate methyl eugenol, 53.45µmol/L 58.27µmol/L, These findings suggest may play dual hispinarum’s host location by sensing from both host, longissimi habitat, i.e.

Язык: Английский

Cloning, Expression, and Odorant Binding Characteristics of the Chemosensory Protein 1 in Asecodes hispinarum, a Parasitoid of the Coconut Leaf Beetle (Brontispa longissima) DOI
Keming Li, Fuping Lu,

Huazhou Wu

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Май 7, 2025

Abstract Asecodes hispinarum (Hymenoptera: Eulophidae) is an endoparasitic wasp and efficient biological control agent of the coconut leaf beetle, Brontispa longissima, a significant pest more than 20 palm species. Due to its effectiveness against B. A. extensively utilized in management palms such as coconut, betel nut, oil palm. Olfaction plays crucial role insect behaviors finding food, selecting oviposition sites, locating mates. However, molecular mechanisms underlying olfactory recognition have not been reported. In previous study, we identified several olfaction-related genes through transcriptome sequencing bioinformatics analysis. current chemosensory protein gene 1 (AhisCSP1) was cloned, real-time PCR employed assess expression levels AhisCSP1 across different developmental stages adult tissues hispinarum. Additionally, odorant binding characteristics were examined using vitro technology fluorescence competitive assays. The results indicated that expressed at all stages, with higher stage compared other stages. Furthermore, specific antennae, no detected head, thorax, abdomen, legs, or wings. A assay revealed can effectively bound volatiles L-α-terpineol, benzyl alcohol, 3-phenylpropionic acid pentyl ester 2-ethyl-1-hexanol, constants 21.26, 25.42, 27.96, 39.72 µmol/L, respectively. Among four longissima larvae, demonstrated strong capacity isohexadecane, constant 19.76 medium pentadecane, 34.28 weak butyl benzoate methyl eugenol, 53.45µmol/L 58.27µmol/L, These findings suggest may play dual hispinarum’s host location by sensing from both host, longissimi habitat, i.e.

Язык: Английский

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