Emu-miR-10a-5p in Echinococcus multilocularis-derived-extracellular vesicles alleviates airway inflammation in mice with allergic asthma by inhibiting macrophage M2a polarization through LIF-mediated JAK1–STAT3 signaling DOI Creative Commons

Yunzhuo Xin,

Rou Wen,

Dong Eun Song

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Май 27, 2025

Introduction Parasites and parasite-derived extracellular vesicles (EVs) microRNAs (miRNAs) can protect against inflammatory diseases, such as asthma. M2a macrophages facilitate the development of allergic miR-10a-5p is closely associated with asthma, emu-miR-10a-5p (encapsulated in Echinococcus multilocularis EVs), shares seed-site sequences mature human mouse miRNAs. Methods We purified EVs by centrifugation, characterized via nanoparticle tracking analysis (NTA) transmission electron microscopy (TEM). used MH-S cells to construct polarization model. The gene expression changes after transfect mimics was analyzed through transcriptome sequencing. established a model ovalbumin (OVA)-induced Hematoxylin eosin (H&E), Masson’s trichrome, periodic acid–Schiff (PAS) staining were detect airway inflammation lung tissues. RT-qPCR, flow cytometry Western Blot assays performed validate related genes proteins. Results Here, we observed that E. -derived their encapsulated transcripts inhibited macrophage polarization. also found targeted leukemia inhibitory factor (LIF) mRNA downstream Janus kinase 1 (JAK1)–signal transducer activator transcription 3 (STAT3)-signaling pathway. asthma alleviated pulmonary mice while inhibiting accumulation macrophages. intervention LIF JAK1–STAT3 signaling lungs Discussion These findings suggest might regulate pathway targeting binding cross-species manner, thereby alleviating enhance current understanding mechanisms underlying immune regulation during infection maintenance stability.

Язык: Английский

Crosstalk between the tumor immune microenvironment and metabolic reprogramming in pancreatic cancer: new frontiers in immunotherapy DOI Creative Commons
Jingjing Shi, Xiaoyan Cui, Junlin Wang

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 28, 2025

In recent years, the incidence and mortality of pancreatic cancer (PC) are increasing year by year. The highly heterogeneous nature PC, its strong immune escape ability easy metastasis make it most lethal malignant tumor in world. With rapid development sequencing technology, complex components microenvironment (TME) PC have been gradually revealed. Interactions between stellate cells, tumor-associated fibroblasts, various types cells collectively promote metabolic reprogramming all cells. This further enhances mechanism ultimately induces to become severely resistant chemotherapy immunotherapy. On one hand, achieve re rational utilization glucose, amino acids lipids through reprogramming, which turn accomplishes biosynthesis energy metabolism requirements. Under such conditions, tumorigenesis, proliferation promoted. other (TIME) also undergo leads progression suppression anti-immune responses inhibiting function normal anti-tumor enhancing immunosuppressive aim this review is explore interaction PC. focus summarize specific mechanisms action addition, will immunotherapy resistance future, targeting provide a solid theoretical basis for combination therapies

Язык: Английский

Процитировано

0
Emu-miR-10a-5p in Echinococcus multilocularis-derived-extracellular vesicles alleviates airway inflammation in mice with allergic asthma by inhibiting macrophage M2a polarization through LIF-mediated JAK1–STAT3 signaling DOI Creative Commons

Yunzhuo Xin,

Rou Wen,

Dong Eun Song

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Май 27, 2025

Introduction Parasites and parasite-derived extracellular vesicles (EVs) microRNAs (miRNAs) can protect against inflammatory diseases, such as asthma. M2a macrophages facilitate the development of allergic miR-10a-5p is closely associated with asthma, emu-miR-10a-5p (encapsulated in Echinococcus multilocularis EVs), shares seed-site sequences mature human mouse miRNAs. Methods We purified EVs by centrifugation, characterized via nanoparticle tracking analysis (NTA) transmission electron microscopy (TEM). used MH-S cells to construct polarization model. The gene expression changes after transfect mimics was analyzed through transcriptome sequencing. established a model ovalbumin (OVA)-induced Hematoxylin eosin (H&E), Masson’s trichrome, periodic acid–Schiff (PAS) staining were detect airway inflammation lung tissues. RT-qPCR, flow cytometry Western Blot assays performed validate related genes proteins. Results Here, we observed that E. -derived their encapsulated transcripts inhibited macrophage polarization. also found targeted leukemia inhibitory factor (LIF) mRNA downstream Janus kinase 1 (JAK1)–signal transducer activator transcription 3 (STAT3)-signaling pathway. asthma alleviated pulmonary mice while inhibiting accumulation macrophages. intervention LIF JAK1–STAT3 signaling lungs Discussion These findings suggest might regulate pathway targeting binding cross-species manner, thereby alleviating enhance current understanding mechanisms underlying immune regulation during infection maintenance stability.

Язык: Английский

Процитировано

0