ACS Applied Engineering Materials, Год журнала: 2024, Номер unknown
Опубликована: Дек. 4, 2024
Язык: Английский
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Июнь 15, 2024
Abstract Virus infectivity is traditionally determined by endpoint titration in cell cultures, and requires complex processing steps human annotation. Here we developed an artificial intelligence (AI)-powered automated framework for ready detection of virus-induced cytopathic effect (DVICE). DVICE uses the convolutional neural network EfficientNet-B0 transmitted light microscopy images infected including coronavirus, influenza virus, rhinovirus, herpes simplex vaccinia adenovirus. robustly measures effects (CPE), as shown class activation mapping. Leave-one-out cross-validation different types demonstrates high accuracy viruses, SARS-CoV-2 saliva. Strikingly, exhibits virus specificity, with adenovirus, herpesvirus, SARS-CoV-2. In sum, provides unbiased scores infectious agents causing CPE, can be adapted to laboratory diagnostics, drug screening, serum neutralization or clinical samples.
Язык: Английский
Процитировано
5
Cell Reports Methods, Год журнала: 2023, Номер 3(9), С. 100557 - 100557
Опубликована: Авг. 17, 2023
Despite advances in virological sciences and antiviral research, viruses continue to emerge, circulate, threaten public health. We still lack a comprehensive understanding of how cells individuals remain susceptible infectious agents. This deficiency is part due the complexity viruses, including cell states controlling virus-host interactions. Microscopy samples distinct cellular infection stages multi-parametric, time-resolved manner at molecular resolution increasingly enhanced by machine learning deep learning. Here we discuss state-of-the-art artificial intelligence (AI) augments light electron microscopy research cells. describe current procedures for image denoising, object segmentation, tracking, classification, super-resolution showcase examples AI has improved acquisition analyses data. The power AI-enhanced will help unravel virus mechanisms, develop agents, improve viral vectors.
Язык: Английский
Процитировано
9
Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 501 - 544
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Янв. 16, 2024
Abstract The organisation of human chromosomes reversibly changes in cell division, and irreversibly apoptosis or erythropoiesis by DNA condensation fragmentation processes. Yet, how viral replication the nucleus affects host chromatin remains poorly understood. Here we used dual-color click chemistry to image adenovirus replication, demonstrating compaction during active expansion compartment (VRC). Early-replicated (vDNA) segregated from VRC lost phospho-serine5-RNA Pol-II DNA-binding protein (DBP), while late-replicated vDNA retained RNA Pol-II, besides RNA-splicing DNA-packaging proteins. Depending on assembly 52K, late-stage VRCs gave rise progeny droplet formation with GFP-tagged virion V into 52K biomolecular condensates. study reveals distinct functions early provides insight passive liquid phase separated zones conducive selective genome packaging nascent virions.
Язык: Английский
Процитировано
1
Lab on a Chip, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
The paper presents a Prusa Mini+ 3D printer's adaptation for lab-in-tubing system with an integration of printed modules, capable producing varied monodispersed droplets from multiple Eppendorf tubes desired volumes and frequency.
Язык: Английский
Процитировано
0
ACS Applied Engineering Materials, Год журнала: 2024, Номер unknown
Опубликована: Дек. 4, 2024
Язык: Английский
Процитировано
0