Single-cell transcriptomics unveils multifaceted immune heterogeneity in early-onset versus late-onset cervical cancer
World Journal of Surgical Oncology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Янв. 14, 2025
Early-onset
(EOCC)
and
late-onset
cervical
cancers
(LOCC)
represent
two
clinically
distinct
subtypes,
each
defined
by
unique
clinical
manifestations
therapeutic
responses.
However,
their
immunological
profiles
remain
poorly
explored.
Herein,
we
analyzed
single-cell
transcriptomic
data
from
4
EOCC
LOCC
samples
to
compare
immune
architectures.
Epithelial
cells
in
exhibited
a
notable
dual
phenotype,
characterized
immune-suppressive
properties
driven
elevated
CXCL
production,
alongside
immune-stimulatory
features
linked
heightened
HLA
molecule
expression.
CD4
+
CD8
T
demonstrated
activation
state,
while
NK
diminished
cytotoxicity.
Macrophages
displayed
enhanced
polarization
towards
both
M1
M2
phenotypes,
along
with
dendritic
showing
augmented
antigen-presenting
capacity.
Regarding
cancer-associated
fibroblasts
(CAFs),
was
enriched
inflammatory
CAFs,
whereas
harbored
higher
proportion
of
CAFs.
These
findings
reveal
the
multifaceted
heterogeneity
between
LOCC,
underscoring
imperative
for
age-tailored
immunotherapeutic
strategies.
Язык: Английский
The Current Status of T Cell Receptor (TCR) Repertoire Analysis in Colorectal Cancer
Hiroyuki Takahashi,
Katsuzo Hanaoka,
Hideo Wada
и другие.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2698 - 2698
Опубликована: Март 17, 2025
The
rapid
increase
in
colorectal
cancer
(CRC)
cases
recently
has
highlighted
the
need
to
use
predictive
biomarkers
guide
therapeutic
approaches.
Current
studies
have
focused
on
tumor-infiltrating
lymphocytes
present
tumor
microenvironment
(TME),
which
cytotoxic
T
cell
activation
and
amount
are
associated
with
CRC
patient
prognosis.
receptor
(TCR)
is
essential
for
antigen
recognition
identification,
playing
a
central
role
immunotherapy.
status
reflects
TCR
diversity
or
clonality,
known
as
repertoire.
Accordingly,
analyzing
repertoire
dynamics
may
help
predict
immunological
circumstances
of
TME
timely
way.
In
this
review,
we
summarize
repertoire-related
knowledge,
including
its
potential
CRC.
intratumoral
restricted
patients
compared
healthy
individuals,
well
peripheral
blood.
Patients
deficient
mismatch
repair
display
more
restriction
than
those
proficient
repair.
Importantly,
higher
before
treatment
decrease
following
indicate
good
response
better
clinical
outcome
patients.
future
sequencing
technology
combined
artificial
intelligence-based
analysis
strategy
decision
making.
Язык: Английский
Colorectal Organoids: Models, Imaging, Omics, Therapy, Immunology, and Ethics
Cells,
Год журнала:
2025,
Номер
14(6), С. 457 - 457
Опубликована: Март 19, 2025
Colorectal
epithelium
was
the
first
long-term
3D
organoid
culture
established
in
vitro.
Identification
of
key
components
essential
for
survival
stem
cell
niche
allowed
an
indefinite
propagation
these
cultures
and
modulation
their
differentiation
into
various
lineages
mature
intestinal
epithelial
cells.
While
methods
were
eventually
adapted
to
establish
organoids
from
different
organs,
colorectal
remain
a
pioneering
model
development
new
applications
health
disease.
Several
basic
applicative
aspects
culture,
modeling,
monitoring
testing
are
analyzed
this
review.
We
also
tackle
ethical
problems
biobanking
distribution
precious
research
tools,
frequently
confined
laboratory
origin
or
condemned
destruction
at
end
project.
Язык: Английский
Decoding the genetic and environmental forces in propelling the surge of early-onset colorectal cancer
Chinese Medical Journal,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 18, 2025
Abstract
Early-onset
colorectal
cancer
(EOCRC)
shows
a
different
epidemiological
trend
compared
to
later-onset
cancer,
with
its
incidence
rising
in
most
regions
and
countries
worldwide.
However,
the
reasons
behind
this
remain
unclear.
The
etiology
of
EOCRC
is
complex
could
involve
both
genetic
environmental
factors.
Apart
from
Lynch
syndrome
Familial
Adenomatous
Polyposis,
sporadic
exhibits
broad
spectrum
pathogenic
germline
mutations,
polymorphisms,
methylation
changes,
chromosomal
instability.
Early-life
exposures
risk
factors,
including
lifestyle
dietary
have
been
found
be
associated
risk.
Meanwhile,
specific
chronic
diseases,
such
as
inflammatory
bowel
disease,
diabetes,
metabolic
syndrome,
EOCRC.
Interactions
between
factors
also
explored.
Here
we
present
findings
narrative
review
studies
on
assessment
early-life
exposures,
EOCRC-specific
their
interactions
susceptible
loci.
We
results
genome-wide
association
that
used
perform
Mendelian
randomization
analyses
ascertain
potential
causal
links
Язык: Английский
Genetic Variation and Regulation of MICA Alters Natural Killer Cell-Mediated Immunosurveillance in Early-Onset Colorectal Cancer
medRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 26, 2024
The
incidence
of
colorectal
cancer
(CRC)
among
individuals
under
age
50,
or
early-onset
CRC
(EOCRC),
has
been
rising
over
the
past
few
decades
for
unclear
reasons,
and
etiology
disease
remains
largely
unknown.
Known
genetic
risk
factors
do
not
explain
this
increase,
pointing
to
possible
environmental
as-yet
unidentified
contributors
their
interactions.
Previous
research
linked
variation
on
chromosome
6
increased
risk.
This
region
harbors
multiple
immune
genes,
including
gene
encoding
Major
Histocompatibility
Complex
(MHC)
class
I
polypeptide-related
sequence
A
(MICA).
MICA
is
a
polygenic
ligand
Natural
Killer
Group
2D
receptor
(NKG2D),
expressed
(NK)
cells
other
lymphocytes.
Given
that
intra-tumoral
NK
cell
infiltration
correlates
with
favorable
outcomes,
we
hypothesized
germline
in
Язык: Английский