
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Ноя. 3, 2024
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has shown wastewater (WW) surveillance to be an effective means of tracking the emergence viral lineages which arrive by many routes transmission including via transportation hubs. In Canadian province Ontario, numerous municipal treatment plants (WWTPs) participate in WW infectious disease targets such as SARS-CoV-2 qPCR and whole genome sequencing (WGS). Greater Toronto Airports Authority (GTAA), operator Pearson International Airport (Toronto Pearson), been participating since January 2022. As a major international airport Canada largest national hub, this is ideal location for globally emerging variants concern (VOCs). study, collected from Pearson’s two terminals pooled aircraft sewage was processed WGS using tiled-amplicon approach targeting virus genome. Data generated analyzed monitor trends lineage frequencies. Initial detections were compared between samples, samples surrounding regions, Ontario clinical data published Public Health Ontario. Results enabled early detection VOCs individual mutations On average, novel at preceded 1–4 weeks, up 16 weeks one case. This project illustrates efficacy transitory hubs sets example that could applied other viruses part preparedness strategy provide monitoring on mass scale.
Язык: Английский