Glutamine induces lateral root initiation, stress responses, and disease resistance in Arabidopsis

PLANT PHYSIOLOGY, Год журнала: 2024, Номер 195(3), С. 2289 - 2308

Опубликована: Март 11, 2024

Abstract The production of glutamine (Gln) from NO3− and NH4+ requires ATP, reducing power, carbon skeletons. Plants may redirect these resources to other physiological processes using Gln directly. However, feeding as the sole nitrogen (N) source has complex effects on plants. Under optimal concentrations, Arabidopsis (Arabidopsis thaliana) seedlings grown have similar primary root lengths, more lateral roots, smaller leaves, higher amounts amino acids proteins compared those NH4NO3. While high levels accumulate in Gln, expression GLUTAMINE SYNTHETASE1;1 (GLN1;1), GLN1;2, GLN1;3 encoding cytosolic GS1 increases GLN2 chloroplastic GS2 decreases. These results suggest that distinct regulating GLN1 gene expression. Notably, an unexpected profile. Compared with NH4NO3, which activates growth-promoting genes, preferentially induces stress- defense-responsive genes. Consistent data, exogenous treatment enhances disease resistance Arabidopsis. induction Gln-responsive including PATHOGENESIS-RELATED1, SYSTEMIC ACQUIRED RESISTANCE DEFICIENT1, WRKY54, WALL ASSOCIATED KINASE1, is compromised salicylic acid (SA) biosynthetic signaling mutants under treatments. Together, partly interact SA pathway trigger plant immunity.

Язык: Английский

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Asymmetry of Motif Conservation Within Their Homotypic Pairs Distinguishes DNA-Binding Domains of Target Transcription Factors in ChIP-Seq Data

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 386 - 386

Опубликована: Янв. 4, 2025

Transcription factors (TFs) are the main regulators of eukaryotic gene expression. The cooperative binding at least two TFs to genomic DNA is a major mechanism transcription regulation. Massive analysis co-occurrence overrepresented pairs motifs for different target studied in ChIP-seq experiments can clarify mechanisms TF cooperation. We categorized from M. musculus and A. thaliana ChIP-seq/DAP-seq according structure their DNA-binding domains (DBDs) into classes. homotypic motifs, using same recognition model each motif. Asymmetric symmetric consist remote close scores. found that asymmetric predominate all murine/plant ‘Basic helix–loop–helix (bHLH)’, leucine zipper (bZIP)’, ‘Tryptophan cluster’ classes murine ‘p53 domain’ ‘Rel homology region’ showed highest enrichment motifs. Pioneer TFs, despite DBD types, have higher significance asymmetry within compared other TFs. Asymmetry CEs promising new feature decrypting

Язык: Английский

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Physiological and biochemical mechanisms in underground vegetable crops for growth and development

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 39 - 53

Опубликована: Янв. 1, 2025

Язык: Английский

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A systematic review to identify target genes that modulate root system architecture in response to abiotic stress

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 17, 2025

Язык: Английский

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MONOPTEROS isoform MP11ir role during somatic embryogenesis in Arabidopsis thaliana

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 21, 2024

Abstract Auxin is crucial for plant morphogenesis, including in vivo and vitro embryo development. Exogenous auxin application necessary inducing embryogenic responses cultured explants of Arabidopsis other plants. Thus, components transport, signaling, metabolism are key to somatic formation. AUXIN RESPONSE FACTOR (ARF) transcription factors, which bind response elements control the auxin-responsive gene expression, vital regeneration. ARFs often repressed by AUXIN/INDOLE-3-ACETIC ACIDs (Aux/IAAs). MONOPTEROS (MP)/ARF5 especially important transition, being highly expressed during embryogenesis; its mutant cannot develop embryos. The MP11ir transcript, an alternatively spliced variant MP/ARF5 , produces a truncated protein missing Phox Bem1p (PB1) domain, ARF-Aux/IAA dimerization. This makes isoform insensitive Aux/IAA repression, suggesting auxin-independent regulation. High levels transcript observed auxin- trichostatin A-dependent induction embryogenesis. Both essential regeneration mpS319 mutant. However, overexpressing (ΔARF5) lacking PB1 domain inhibits embryogenesis, leading callus formation instead Overexpressing ΔARF5 ( mp/arf5 mutant), blocking action with auxin-resistant BODENLOS (BDL) affect expression genes involved biosynthesis, like TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 TAA1 ), TAA1-RELATED TAR1 YUCCA3 YUC3 YUC5 YUC8 may be potential targets and/or MP/ARF5. Consequently, overexpression alters homeostasis endogenous levels, hindering transition.

Язык: Английский

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Elucidating the callus-to-shoot-forming mechanism in Capsicum annuum ‘Dempsey’ through comparative transcriptome analyses

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Май 7, 2024

Abstract Background The formation of shoots plays a pivotal role in plant organogenesis and productivity. Despite its significance, the underlying molecular mechanism de novo regeneration has not been extensively elucidated Capsicum annuum ‘Dempsey’, bell pepper cultivar. To address this, we performed comparative transcriptome analysis focusing on differential expression C. ‘Dempsey’ shoot, callus, leaf tissue. We further investigated phytohormone-related biological processes their interacting genes based transcriptomic across five species. Results provided comprehensive view gene networks regulating shoot revealing strong involvement hypoxia responses oxidative stress. Our revealed significant conservation increase patterns related to auxin defense mechanisms both callus tissues. Consequently, response emerged as critical regulators ‘Dempsey’. Current data also indicated substantial decline linked photosynthesis within regenerative tissues, implying deactivation regulatory system governing Conclusion Coupled with mechanisms, thus considered spatial redistribution play morphogenesis via primordia outgrowth. findings shed light explants, important information for programs, have broader implications precise breeding recalcitrant crops.

Язык: Английский

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The role of auxin-mediated gene activation in the bryophyte, Physcomitrium patens

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 17, 2024

Abstract Perception and response to the hormone auxin is critical plant growth development. Expression of auxin-response genes tightly regulated via known mechanisms both activation repression. Across lineage, gene induction performed by AUXIN-REPSONSE FACTOR (ARF) activating transcription factors. Conversely, AUXIN/INDOLE ACETIC ACID proteins repress expression. Studies gain-of-function constitutive-repression lines ARF loss-of-function mutants have advanced field. Yet, there a need for comparative study aberrant auxin-signaling understand developmental consequences constitutive repression versus absence auxin-mediated induction. Using CRISPR/Cas9 gene-editing tools, we mutated each in model bryophyte, Physcomitrium patens. The resulting septuple mutant line ( arfa sept ) has severe phenotypes diminished ability respond exogenous auxin. However, phenotypic analysis revealed that not as severely affected lines. several demonstrate abolished but basal expression levels are higher Our results suggest auxin-regulated important progression maintained, albeit at reduced levels, ARFs. Highlight Researchers used CRIPSR/Cas9 produce Auxin Response Factor bryophyte patens revealing complete loss on

Язык: Английский

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Tomato SlARF5 participate in the flower organ initiation process and control plant height

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Окт. 23, 2024

Plant height is a critical agronomic trait closely linked to yield, primarily regulated by Gibberellins (GA) and auxins, which interact in complex ways. However, the mechanism underlying their interactions remain incompletely understood. In this study, we identified tomato mutant exhibiting significantly reduced plant height. Through gene cloning bulked segregant analysis (BSA) sequencing, found that corresponds auxin response factor SlARF5/MP. Here, show overexpression of SlARF5/MP enhances Additionally, treatment with GA3 restored wild-type (WT) levels, indicating GA content key influencing We also observed significant upregulation GA-biosynthesis genes, including GA2-oxidases GA20ox3 GA20ox4, as well biosynthesis GA3ox1, SlARF5-overexpressing plants. Furthermore, demonstrated SlARF5 directly binds SlGA2ox3, mediates conversion inactive GA, therebyregulating its expression. Our findings suggest modulates metabolism regulating synthesis ultimately leading alterations

Язык: Английский

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Genome-wide characterization of auxin response factor (ARF) genes in bermudagrass and ectopically functional analysis of CdARF6-B2 gene in Arabidopsis

Physiology and Molecular Biology of Plants, Год журнала: 2024, Номер 30(12), С. 1969 - 1981

Опубликована: Дек. 1, 2024

Язык: Английский

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Organogenesis as the Realization of the Morphogenetic Potential of Callus Cells in In Vitro Conditions

Russian Journal of Developmental Biology, Год журнала: 2024, Номер 55(3), С. 140 - 152

Опубликована: Июнь 1, 2024

Язык: Английский

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