Regulatory and retrograde signaling networks in the chlorophyll biosynthetic pathway

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 24, 2025

ABSTRACT Plants, algae and photosynthetic bacteria convert light into chemical energy by means of photosynthesis, thus providing food for most organisms on Earth. Photosynthetic pigments, including chlorophylls (Chls) carotenoids, are essential components that absorb the necessary to drive electron transport in photosynthesis. The biosynthesis Chl shares several steps common with other tetrapyrroles, siroheme, heme phycobilins. Given many tetrapyrrole precursors possess photo‐oxidative properties deleterious macromolecules can lead cell death, (TBS) requires stringent regulation under various developmental environmental conditions. Thanks decades research model plants algae, we now have a deeper understanding regulatory mechanisms underlie synthesis, (i) factors control activity stability TBS enzymes, (ii) transcriptional post‐translational pathway, (iii) complex roles tetrapyrrole‐mediated retrograde signaling from chloroplasts cytoplasm nucleus. Based these new findings, Chls their derivatives will find broad applications synthetic biology agriculture future.

Язык: Английский

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Chlorophyll Degradation and its Physiological Function

Plant and Cell Physiology, Год журнала: 2024, Номер unknown

Опубликована: Авг. 22, 2024

Research on chlorophyll degradation has progressed significantly in recent decades. In the 1990s, structure of linear tetrapyrrole, which is unambiguously a product, was determined. From 2000s until 2010s, major enzymes involved were identified, and pheophorbide oxygenase/phyllobilin pathway established. This encompasses several steps: (1) initial conversion b to 7-hydroxymethyl a; (2) (3) dechelation pheophytin (4) dephytylation (5) opening macrocycle yield red catabolite; (6) catabolite phyllobilins. converts potentially harmful into safe molecules phyllobilins, are stored central vacuole terrestrial plants. The expression chlorophyll-degrading mediated by various transcription factors influenced light conditions, stress, plant hormones. Chlorophyll differently regulated different organs developmental stages initiation induces further enzymes, resulting acceleration degradation. initially considered last reaction senescence; however, plays crucial roles enhancing senescence, degrading chlorophyll-protein complexes, forming photosystem II, maintaining seed quality. Therefore, controlling important agricultural applications.

Язык: Английский

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Identification and functional characterization of the MYB transcription factor GmMYBLJ in soybean leaf senescence

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Янв. 24, 2025

Leaf senescence is an important agronomic trait that significantly influences the quality and yield of soybeans. v-Myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors are considered crucial regulators governing leaf senescence, which can be utilized to improve traits in crops. However, our knowledge regarding functional roles soybean MYBs extremely limited. In this study, GmMYBLJ , a CCA1-like MYB was identified functionally characterized with respect senescence. The protein localized nucleus, high accumulation its transcripts observed nodules embryos. Notably, highly expressed senescent leaves transcriptionally induced by dark or NaCl treatment, as confirmed histochemical GUS staining analysis. Ectopic overexpression Arabidopsis not only led earlier reduced chlorophyll content, increased MDA but also promoted expression several WRKY family senescence-associated genes, such SAG12 ORE1 . Further investigation showed accelerated under darkness response Pst DC3000 infection. Moreover, transgenic plants overexpressing grew faster exhibited salt stress. DAB analysis ROS hairy roots leaves. Collectively, results provided useful information into both age-dependent stress-induced

Язык: Английский

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The Mulberry WRKY Transcription Factor MaWRKYIIc7 Participates in Regulating Plant Drought Stress Tolerance

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1714 - 1714

Опубликована: Фев. 17, 2025

The sericulture industry is an important component of the agricultural industry. Drought stress can cause yellowing, premature ageing, and shrinkage mulberry (Morus alba L.) leaves, greatly damaging their quality restricting high-quality development WRKY transcription factors play roles in plant drought response. In this study, we found that MaWRKYIIc7 TFs, had significantly higher expression levels leaves than other tissues was induced to be expressed under stress. protein located nucleus plasma membrane, its transcriptional activity depends mainly on N-terminal sequence. overexpression Arabidopsis resulted better tolerance. An analysis transient seedlings revealed transgenic presented decreased stomatal opening, MDA content, increased ROS clearance ability, ABA biosynthesis-related genes. Y1H Dual-luc results indicate bind W-boxes positively regulate MaNCED1 MaRD29A, synergistically regulating tolerance mulberry. Overall, our research suggests increase by promoting clearance, adjusting activating signalling pathway.

Язык: Английский

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HLS1 interacts with ATG8 to negatively regulate the ABS3-mediated plant senescence pathway

Cell Reports, Год журнала: 2025, Номер 44(4), С. 115507 - 115507

Опубликована: Апрель 1, 2025

In Arabidopsis, late endosome-localized ABNORMAL SHOOT 3 (ABS3) promotes senescence through a direct interaction with AUTOPHAGY8 (ATG8). However, the molecular mechanisms underlying regulation of ABS3-mediated pathway are not well understood. Here, we report that HOOKLESS 1 (HLS1) acts as negative regulator plant and pathway. We identify localizations HLS1 at plasma membrane endosomes in addition to nucleus. Mechanistically, non-nucleus-localized directly interacts ATG8, attenuates ABS3-ATG8 interaction, inhibits vacuolar degradation ABS3, thereby antagonizing senescence-promoting role ABS3. Additionally, show stability itself is negatively regulated during carbon deprivation-induced senescence. Finally, find homologs both Arabidopsis wheat play conserved regulation. summary, our findings functional link between HLS1, ATG8

Язык: Английский

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AtALMT9 Modulates Leaf Development and Disease Resistance in Arabidopsis thaliana

Plant Cell & Environment, Год журнала: 2025, Номер unknown

Опубликована: Май 20, 2025

ABSTRACT Delaying plant aging and death enhancing immune defenses of have beneficial effects on crop production the continued development agricultural economy. In this study, we focused AtALMT9, which is located in vacuolar membrane. Specifically, investigated its functions Arabidopsis thaliana leaf disease resistance. Our physiological analysis revealed that AtALMT9 negatively regulates response to pathogenic microorganisms. Moreover, RNA‐seq qRT‐PCR analyses indicated aging‐ immunity‐related genes were significantly differentially expressed almt9 mutant plants. Furthermore, impaired accelerated resistance due decreased contents chlorophyll malic acid as well increased levels salicylic jasmonic acid. This study provides new findings molecular mechanism underlying involvements resistance, potentially providing a theoretical basis for productivity.

Язык: Английский

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Methodological pipeline for monitoring post-harvest quality of leafy vegetables

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Ноя. 23, 2023

Abstract Plants are primary source of nutrients for humans. However, the nutritional value vegetables tends to decrease once organ and tissue sinks detached from plant. Minimal processing leafy involves cutting washing before packaging storage. These procedures result in stressful conditions post-harvest disorders senescence-related can also occur. The aim this work is define a methodological pipeline evaluate “quality” changes fresh cut over their shelf-life. At purpose, intra-species variability has been investigated considering two varieties Lactuca sativa (var. longifolia capitata ), showing different susceptibility browning. Since browning mainly depends on phenol oxidation, redox parameters as well activity enzymes involved biosynthesis oxidation have monitored storage time. same time, metabolic lettuce leaves estimated response patterns chemical sensors. obtained sensor outputs were predictive browning-related biological features cultivar-dependent manner. integration results by multivariate approach allowed identification most appropriate quality markers varieties. This proposed subsequent monitoring vegetables.

Язык: Английский

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Endoplasmic reticulum protein ALTERED MERISTEM PROGRAM 1 negatively regulates senescence in Arabidopsis

PLANT PHYSIOLOGY, Год журнала: 2024, Номер 196(1), С. 273 - 290

Опубликована: Май 23, 2024

Abstract Plant senescence is a highly regulated developmental program crucial for nutrient reallocation and stress adaptation in response to environmental cues. Stress-induced age-dependent natural share both overlapping distinct molecular responses regulatory schemes. Previously, we have utilized carbon-deprivation (C-deprivation) assay using Arabidopsis (Arabidopsis thaliana) seedlings investigate regulation. Here conducted comprehensive time-resolved transcriptomic analysis of wild type subjected C-deprivation treatment at multiple time points, unveiling substantial temporal changes gene expression patterns. Moreover, identified ALTERED MERISTEM PROGRAM 1 (AMP1), encoding an endoplasmic reticulum protein, as potential regulator based on its profile. By characterizing loss-of-function alleles overexpression lines AMP1, confirmed role negative plant senescence. Genetic analyses further revealed synergistic interaction between AMP1 the autophagy pathway regulating Additionally, discovered functional association endosome-localized ABNORMAL SHOOT3 (ABS3)-mediated positioned key senescence-promoting transcription factors downstream AMP1. Overall, our findings shed light intricacies transcriptome reprogramming during C-deprivation-induced interplay among endomembrane compartments controlling

Язык: Английский

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Transcriptome-Wide Identification of Dark- and Salt-Induced Senescence-Related NAC Gene Family Members in Alfalfa

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(16), С. 8908 - 8908

Опубликована: Авг. 15, 2024

Leaves are a key forage part for livestock, and the aging of leaves affects biomass quality. Preventing or delaying premature leaf senescence leads to an increase in pasture accumulation improvement alfalfa NAC transcription factors have been reported affect plant growth abiotic stress responses. In this study, 48 genes potentially associated with were identified under dark salt conditions. A phylogenetic analysis divided MsNACs into six subgroups based on similar gene structure conserved motif. These unevenly distributed 26 chromosomes. The results collinearity show that all involved duplication. Some cis-acting elements related hormones screened 2-kb promoter regions MsNACs. Nine MsNAC subjected qRT-PCR quantify their expression Agrobacterium-mediated transient verify functions. indicate Ms.gene031485, Ms.gene032313, Ms.gene08494, Ms.gene77666 might be senescence. Our findings extend understanding regulatory function

Язык: Английский

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Genome-Wide Identification and Analysis of the WRKY Transcription Factor Family Associated with Leaf Senescence in Alfalfa

Plants, Год журнала: 2024, Номер 13(19), С. 2725 - 2725

Опубликована: Сен. 29, 2024

Leaves are the most significant parts of forage crops such as alfalfa. Senescence is terminal stage leaf development and controlled by an integrated myriad endogenous signals environmental stimuli. WRKY transcription factors (TFs) play essential roles in regulating senescence; however, only a few studies on analysis identification TF family Medicago Sativa have been reported. In this study, we identified 198 members from alfalfa (M. sativa L.) cultivar ’XinjiangDaye’ using phylogenetic categorized them into three subfamilies, Groups I, II, III, based their structural characteristics. Group II were further divided five subclasses. addition, several hormone- stress-related cis-acting elements promoter regions MsWRKYs. Furthermore, 14 aging-related MsWRKYs genes previous transcriptome our laboratory selected for RT-qPCR validation expression patterns, subsequently cloned overexpression examination. Finally, MsWRKY5, MsWRKY66, MsWRKY92, MsWRKY141 confirmed to cause yellowing Nicotiana benthaminana transient system. Our findings lay groundwork mechanism M. aging creation new germplasm resources.

Язык: Английский

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