Loss-of-Function RUP-Variants Influence Ubiquitin-Proteasome System and Enhance Carotenoid and Folate Levels in Tomato DOI Creative Commons
Chaitanya Charakana, Suresh K. Gupta,

Anusha Sunkum

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 22, 2025

Abstract REPRESSOR OF UV-B PHOTOMORPHOGENESIS (RUP) negatively regulates UV- B signalling, yet its broader physiological roles in crop plants remain largely unexplored. We compared two tomato accessions bearing truncated RUP proteins— rup-1 and rup-2 ( rup- variants)—with the cultivar Arka Vikas (AV), which harbours native protein. Seedlings of rup -variants exhibited enhanced tolerance to supplemental light. Red ripe (RR) fruits showed significantly elevated carotenoid folate levels AV. Introgression into AV confirmed that increased accumulation is genetically linked truncation. Metabolomic profiling revealed a substantial shift primary metabolic homeostasis, particularly at breaker stage, marked by pronounced reduction sugars amino acids. Proteomic analyses - variants across ripening stages identified significant proportion differentially expressed proteins belonged chaperones ubiquitin-proteasome system (UPS). Upregulation four key enzymes biosynthesis pathway likely contributed lycopene content -variants. Elevated were associated with upregulation C1 metabolism enzymes. Despite widespread reprogramming -variants, hormonal regulatory pathways remained unaltered. Our results suggest modulates during fruit ripening, loss triggers folate/carotenoid levels.

Язык: Английский

Advances in plant photobiology: let’s light it up once again DOI
Sourav Datta, Madhusmita Panigrahy, Kishore C. S. Panigrahi

и другие.

Journal of Experimental Botany, Год журнала: 2025, Номер 76(3), С. 641 - 645

Опубликована: Фев. 7, 2025

Язык: Английский

Процитировано

0
Mechanistic insights on physiological, biochemical, and metabolite profiling of pearl millet cultivars focusing bioactive compounds under elevated UV-B radiation DOI
Pooja Singh, Krishna Kumar Choudhary

Plant Physiology and Biochemistry, Год журнала: 2025, Номер 222, С. 109682 - 109682

Опубликована: Фев. 20, 2025

Язык: Английский

Процитировано

0
Loss-of-Function RUP-Variants Influence Ubiquitin-Proteasome System and Enhance Carotenoid and Folate Levels in Tomato DOI Creative Commons
Chaitanya Charakana, Suresh K. Gupta,

Anusha Sunkum

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 22, 2025

Abstract REPRESSOR OF UV-B PHOTOMORPHOGENESIS (RUP) negatively regulates UV- B signalling, yet its broader physiological roles in crop plants remain largely unexplored. We compared two tomato accessions bearing truncated RUP proteins— rup-1 and rup-2 ( rup- variants)—with the cultivar Arka Vikas (AV), which harbours native protein. Seedlings of rup -variants exhibited enhanced tolerance to supplemental light. Red ripe (RR) fruits showed significantly elevated carotenoid folate levels AV. Introgression into AV confirmed that increased accumulation is genetically linked truncation. Metabolomic profiling revealed a substantial shift primary metabolic homeostasis, particularly at breaker stage, marked by pronounced reduction sugars amino acids. Proteomic analyses - variants across ripening stages identified significant proportion differentially expressed proteins belonged chaperones ubiquitin-proteasome system (UPS). Upregulation four key enzymes biosynthesis pathway likely contributed lycopene content -variants. Elevated were associated with upregulation C1 metabolism enzymes. Despite widespread reprogramming -variants, hormonal regulatory pathways remained unaltered. Our results suggest modulates during fruit ripening, loss triggers folate/carotenoid levels.

Язык: Английский

Процитировано

0