STK19 drives transcription-coupled repair by stimulating repair complex stability, RNA Pol II ubiquitylation, and TFIIH recruitment

Molecular Cell, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Transcription-coupled nucleotide excision repair (TC-NER) efficiently eliminates DNA damage that impedes gene transcription by RNA polymerase II (RNA Pol II). TC-NER is initiated the recognition of lesion-stalled CSB, which recruits CRL4

Язык: Английский

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Coordination of transcription-coupled repair and repair-independent release of lesion-stalled RNA polymerase II

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 17, 2024

Transcription-blocking lesions (TBLs) stall elongating RNA polymerase II (Pol II), which then initiates transcription-coupled repair (TCR) to remove TBLs and allow transcription recovery. In the absence of TCR, eviction lesion-stalled Pol is required for alternative pathways address damage, but mechanism unclear. Using Protein-Associated DNA Damage Sequencing (PADD-seq), this study reveals that p97-proteasome pathway can evict independently repair. Both TCR repair-independent require CSA ubiquitination. However, p97 dispensable in TCR-proficient cells, highlighting repair's prioritization over eviction. Moreover, ubiquitination RPB1-K1268 important both pathways, with USP7's deubiquitinase activity promoting without abolishing release. summary, elucidates fate II, may shed light on molecular basis genetic diseases caused by defects genes.

Язык: Английский

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RNA Polymerase II Activity Control of Gene Expression and Involvement in Disease

Journal of Molecular Biology, Год журнала: 2024, Номер 437(1), С. 168770 - 168770

Опубликована: Авг. 28, 2024

Gene expression is dependent on RNA Polymerase II (Pol II) activity in eukaryotes. In addition to determining the rate of synthesis for all protein coding genes, Pol serves as a platform recruitment factors and regulation co-transcriptional events, from processing chromatin modification remodeling. The transcriptome can be shaped by changes kinetics affecting itself or because alterations events that are responsive coupled with transcription. Genetic, biochemical, structural approaches model organisms have revealed critical insights into how works types regulate it. complexity generally increases organismal complexity. this review, we describe fundamental aspects shape gene expression, discuss recent advances elongation regulated altered function linked human disease aging.

Язык: Английский

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Transcription-coupled repair of DNA–protein crosslinks

Trends in Cell Biology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

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