Current Biology, Год журнала: 2020, Номер 31(1), С. 207 - 213.e4
Опубликована: Окт. 30, 2020
Язык: Английский
Cell Reports, Год журнала: 2024, Номер 43(3), С. 113843 - 113843
Опубликована: Фев. 23, 2024
Whole-body regeneration requires the ability to produce full repertoire of adult cell types. The planarian Schmidtea mediterranea contains over 125 types, which can be regenerated from a stem population called neoblasts. Neoblast fate choice regulated by expression fate-specific transcription factors (FSTFs). How choices are made and distributed across neoblasts versus their post-mitotic progeny remains unclear. We used single-cell RNA sequencing systematically map in S/G
Язык: Английский
Процитировано
19
Development, Год журнала: 2019, Номер 146(17)
Опубликована: Сен. 1, 2019
ABSTRACT Planarians are a group of flatworms. Some planarian species have remarkable regenerative abilities, which involve abundant pluripotent adult stem cells. This makes these worms powerful model system for understanding the molecular and evolutionary underpinnings regeneration. By providing succinct overview taxonomy, anatomy, available tools orchestration regeneration, this Primer aims to showcase both unique assets questions that can be addressed with system.
Язык: Английский
Процитировано
114
Nature Communications, Год журнала: 2019, Номер 10(1)
Опубликована: Апрель 8, 2019
Abstract Regeneration and tissue turnover require new cell production positional information. Planarians are flatworms capable of regenerating all body parts using a population stem cells called neoblasts. The information required for patterning is primarily harbored by muscle cells, which also control contraction. Here we produce an in silico planarian matrisome use recent whole-animal single-cell-transcriptome data to determine that major source extracellular matrix (ECM). No other ECM-secreting, fibroblast-like type was detected. Instead, express core ECM components, including 19 collagen-encoding genes. Inhibition muscle-expressed hemicentin-1 ( hmcn-1 ), encodes highly conserved glycoprotein, results ectopic peripheral localization neoblasts, outside the layer. secretion hmcn-1- dependent maintenance separation indicate functions as connective tissue, raising possibility broad roles adult
Язык: Английский
Процитировано
78
Cell Reports, Год журнала: 2021, Номер 34(9), С. 108803 - 108803
Опубликована: Март 1, 2021
Cell types are the basic building units of multicellular life, with extensive diversities. The evolution cell is a crucial layer comparative biology but thus far not comprehensively studied. We define compendium atlases using single-cell RNA-seq (scRNA-seq) data from seven animal species and construct cross-species cell-type evolutionary hierarchy. present roadmap for origin diversity major categories find that muscle neuron cells conserved types. Furthermore, we identify transcription factor (TF) repertoire specifies categories. Overall, our study reveals conservation divergence during evolution, which will further expand landscape genomics.
Язык: Английский
Процитировано
76
Cell stem cell, Год журнала: 2021, Номер 28(7), С. 1307 - 1322.e5
Опубликована: Апрель 21, 2021
Язык: Английский
Процитировано
75
Nature Genetics, Год журнала: 2022, Номер 54(7), С. 1051 - 1061
Опубликована: Июль 1, 2022
Язык: Английский
Процитировано
62
Cell, Год журнала: 2023, Номер 186(17), С. 3606 - 3618.e16
Опубликована: Июль 21, 2023
Язык: Английский
Процитировано
30
Nature Ecology & Evolution, Год журнала: 2023, Номер 7(12), С. 2108 - 2124
Опубликована: Окт. 19, 2023
Regenerative abilities vary dramatically across animals. Even amongst planarian flatworms, well-known for complete regeneration from tiny body fragments, some species have restricted while others are almost entirely incompetent. Here, we assemble a diverse live collection of 40 to probe the evolution head in group. Combining quantification species-specific head-regeneration with comprehensive transcriptome-based phylogeny reconstruction, show multiple independent transitions between robust whole-body and freshwater species. RNA-mediated genetic interference inhibition canonical Wnt signalling interference-sensitive bypassed all defects, suggesting that pathway is linked emergence defects. Our finding has roles reproductive system model Schmidtea mediterranea raises possibility trade-off egg-laying, asexual reproduction by fission/regeneration drives regenerative trait evolution. Although quantitative comparisons levels, yolk content strategy our remained inconclusive, they revealed divergent planarians. Altogether, study establishes planarians as taxon comparative research presents framework mechanistic abilities.
Язык: Английский
Процитировано
29
Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(26)
Опубликована: Июнь 18, 2024
Germ cells are regulated by local microenvironments (niches), which secrete instructive cues. Conserved developmental signaling molecules act as niche-derived regulatory factors, yet other types of niche signals remain to be identified. Single-cell RNA-sequencing sexual planarians revealed expressing a nonribosomal peptide synthetase (
Язык: Английский
Процитировано
8
Science, Год журнала: 2020, Номер 369(6511), С. 1649 - 1653
Опубликована: Сен. 25, 2020
Schistosome parasites kill 250,000 people every year. Treatment of schistosomiasis relies on the drug praziquantel. Unfortunately, a scarcity molecular tools has hindered discovery new targets. Here, we describe large-scale RNA interference (RNAi) screen in adult
Язык: Английский
Процитировано
62