Efficacy of cleaning, disinfection and sterilization modalities for addressing infectious drug-resistant fungi: A review

Journal of Applied Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 7, 2025

Abstract This is a timely and important review that focuses on the appropriateness of established cleaning, disinfection sterilization methods to safely effectively address infectious fungal drug-resistant pathogens can potentially contaminate reusable medical devices used in healthcare environment order mitigate risk patient infection. The release World Health Organisation (WHO) priority pathogen list (FPPL) 2022 highlighted public health crisis antimicrobial resistance (AMR) clinically relevant species. Contamination with (including those FPPL) are rare events more likely occur due cross-transmission arising from lapses hand-hygiene practices. Established decontaminate single use devices; however, there assumptions destined for semi-critical appropriately cleaned do not harbour biofilms may undermine ability decontamination these type healthcare. International standards dictate manufacturer's instructions must provide appropriate guidance facilities meet safe reprocessing expectations includes addressing drug resistant pathogens. Increased environmental monitoring vigilance surrounding advised including adherence hand hygiene/aseptic practices cleaning encompassing simplification device features ‘ease-of-reach’. There need promote integrated multi-actor hub approach sophisticated challenges future artificial intelligence machine learning improved diagnostics, monitoring/surveillance (such as wastewater-based epidemiology), sterility assurance design. knowledge gap occurrence potential persistence harboured biofilms, particularly ascertaining efficacy high-level devices.

Язык: Английский

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Update on diagnosis and treatment of fungal meningitis: lessons from recent outbreaks

Current Opinion in Infectious Diseases, Год журнала: 2024, Номер 37(6), С. 437 - 442

Опубликована: Сен. 3, 2024

Purpose of review Recently, fungal meningitis outbreaks have occurred in association with neuraxial and epidural anesthesia immunocompetent patients. Herein, we describe the course those outbreaks, their diagnosis, treatment, prognosis, lessons learned. Recent findings Two Fusarium solani during 2022–2023 were associated two distant cities Mexico (Durango Matamoros). The initial etiological agent identification was delayed due to insensitivity cultures. A qPCR validated positive 38% cerebrospinal fluid (CSF) samples from Durango, while BD-Glucan allowed early diagnosis index case Matamoros. Antifungal treatment voriconazole liposomal amphotericin B (L-AmB) recommended. Overall mortality 51%. Once cause confirmed, some patients received fosmanogepix. Summary Fungal filamentous fungi are usually direct inoculation. They result severe presentations high mortality. Early should be suspected, CSF testing screening is Aggressive antifungal based on susceptibility administered as possible. advent molecular diagnostic methods new drugs may allow for timely increasing chances survival.

Язык: Английский

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Clinical Mycology Today: Emerging Challenges and Opportunities

Open Forum Infectious Diseases, Год журнала: 2024, Номер 11(7)

Опубликована: Июнь 27, 2024

Abstract The Mycoses Study Group Education and Research Consortium is a collective of clinicians, researchers, educators with the common goal to advance awareness, diagnosis, management invasive fungal diseases. Clinical Mycology Today, Consortium's biennial meeting, dedicated discussing most pressing contemporary issues facing field clinical mycology, promoting clinical, translational, basic science collaborations, mentoring next generation mycologists. Here, we review current opportunities challenges mycology that arose from discussions at 2022 emphasis on novel host risk factors, emerging resistant pathogens, evolving antifungal pipeline, critical affecting advancement research.

Язык: Английский

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Dispensable genome and segmental duplications drive the genome plasticity in Fusarium solani

Frontiers in Fungal Biology, Год журнала: 2025, Номер 6

Опубликована: Фев. 5, 2025

Fusarium solani is a species complex encompassing large phylogenetic clade with diverse members occupying varied habitats. We recently reported unique opportunistic F. associated unusual dark galls in sugarbeet. assembled the chromosome-level genome of sugarbeet isolate strain SB1 using Oxford Nanopore and Hi-C sequencing. The average size genomes 54 Mb, whereas has larger 59.38 organized into 15 chromosomes. expansion due to high repeats segmental duplications within its three potentially accessory These chromosomes are absent closest reference assembly, vanettenii 77-13-4. Segmental were found but most extensive between two specific chromosomes, suggesting that this may have doubled genes. Further comparison demonstrates inversions syntenic regions an chromosome pan-genome 12 publicly available isolates nearly reached gene saturation, few new genes discovered after addition last genome. Based on orthogroups nucleotide identity, not grouped by lifestyle or origin. analysis further revealed enrichment several enzymes-coding dispensable (accessory + genes) genome, such as hydrolases, transferases, oxidoreductases, lyases, ligases, isomerase, dehydrogenase. evidence presented here suggests plasticity, genetic diversity, adaptive traits driven significant contributions from duplications.

Язык: Английский

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Aseptic Meningitis: Fever, Headache, and a Stiff Neck…Not Looking So Sick

Опубликована: Янв. 1, 2025

Язык: Английский

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Public health trends in neurologically relevant infections: a global perspective

Therapeutic Advances in Infectious Disease, Год журнала: 2024, Номер 11

Опубликована: Янв. 1, 2024

Neuroinfectious diseases represent a growing threat to public health globally. Infections of the central nervous system remain challenging diagnose and treat, partially driven by fact that high proportion emerging pathogens are capable causing neurological disease. Many trends driving emergence novel pathogens, including climate change, ecological degradation, urbanization, global travel, have accelerated in recent years. These circumstances raise concern for potential additional pandemic coming years, necessitating stronger understanding forces give rise spread neuroinvasive commitment infrastructure identify treat these diseases. In this review, we discuss clinical epidemiological features three types significant consequences emblematic key ongoing health. We first dengue viruses context considering environmental factors allow expansion geographic range seasonal population viruses' vector. then review rising prevalence fungal meningitis secondary medical tourism, trend representative highly globalized nature modern healthcare. Lastly, increasing antibiotic-resistant infections intersection antibiotic overuse agricultural settings. Taken together, conditions necessitates recommitment investment focused on local infectious disease surveillance coupled with development therapeutics vaccines pathogens. Such threats also obviate need address root causes diseases, sustained effort anthropogenic change degradation.

Язык: Английский

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Editorial: Emerging fungal pathogens: perspectives

Frontiers in Fungal Biology, Год журнала: 2024, Номер 5

Опубликована: Фев. 14, 2024

EDITORIAL article Front. Fungal Biol., 14 February 2024Sec. Pathogenesis Volume 5 - 2024 | https://doi.org/10.3389/ffunb.2024.1369062

Язык: Английский

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Dispensable genome and segmental duplications drive the genome plasticity inFusarium solani

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 5, 2024

Abstract Fusarium solani is a species complex encompassing large phylogenetic clade with diverse members occupying varied habitats. We recently reported unique opportunistic F. associated unusual dark galls in sugarbeet. assembled the chromosome-level genome of sugarbeet isolate strain SB1 using Oxford Nanopore and Hi-C sequencing. has (59.38 Mb) organized into 15 chromosomes. The expansion due to high repeats massive segmental duplications within its three potentially accessory These chromosomes are absent closest reference assembly, vanettenii 77-13-4. extensive between two suggest that this may have doubled genes. Further comparison demonstrates inversions syntenic regions an chromosome pan-genome 12 publicly available isolates nearly reached gene saturation, few new genes discovered after addition last genome. Based on orthogroups average nucleotide identity, not grouped by lifestyle or origin. analysis further revealed enrichment several enzymes-coding dispensable (accessory + genes) genome, such as hydrolases, transferases, oxidoreductases, lyases, ligases, isomerase, dehydrogenase. evidence presented here suggests plasticity, genetic diversity, adaptive traits driven significant contributions from duplications.

Язык: Английский

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Outcomes in solid organ transplant recipients receiving organs from a donor with Fusarium solani species complex meningitis

Transplant Infectious Disease, Год журнала: 2024, Номер 26(5)

Опубликована: Июль 16, 2024

Abstract Background Five organs (heart, right lung, liver, right, and left kidneys) from a deceased patient were transplanted into five recipients in four US states; the was identified as part of healthcare‐associated fungal meningitis outbreak among patients who underwent epidural anesthesia Matamoros, Mexico. Methods After transplant surgeries occurred, Fusarium solani species complex, pathogen with high case‐mortality rate, cerebrospinal fluid organ donor by metagenomic next‐generation sequencing (mNGS) fungal‐specific polymerase chain reaction plasma mNGS. Results Four received recommended voriconazole prophylaxis; monitored weekly serum (1‐3)‐β‐ d ‐glucan testing. All for signs infection at least 3 months following transplantation. The liver recipient had graft failure, which attributed to an etiology unrelated infection. No DNA sections explanted suggesting that F. complex did not contribute failure. remaining experienced no or symptoms suggestive fusariosis. Conclusion Antifungal prophylaxis may be useful preventing donor‐derived infections donors are found have meningitis. image

Язык: Английский

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An Update on Fungal Disease Outbreaks of Public Health Concern

Infectious Disease Clinics of North America, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

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