The Plant Cell, Год журнала: 2023, Номер 36(2), С. 223 - 224
Опубликована: Окт. 31, 2023
Язык: Английский
Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(28)
Опубликована: Июль 5, 2024
Bioengineering of plant immune receptors has emerged as a key strategy for generating novel disease resistance traits to counteract the expanding threat pathogens global food security. However, current approaches are limited by rapid evolution in field and may lack durability when deployed. Here, we show that rice nucleotide-binding, leucine-rich repeat (NLR) receptor Pik-1 can be engineered respond conserved family effectors from multihost blast fungus pathogen Magnaporthe oryzae . We switched effector binding response profile Pik NLR its cognate AVR-Pik host-determining factor pathogenicity toward weeping lovegrass 2 (Pwl2) installing putative host target, OsHIPP43, place native integrated heavy metal–associated domain (generating Pikm-1 OsHIPP43 ). This chimeric also responded other PWL alleles diverse isolates. The crystal structure Pwl2/OsHIPP43 complex revealed multifaceted, robust interface cannot easily disrupted mutagenesis, therefore provide durable, broad isolates carrying field. Our findings highlight how targets used bioengineer recognition specificities have more properties compared naturally evolved genes.
Язык: Английский
Процитировано
13
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Ноя. 15, 2024
Nucleotide-binding leucine-rich repeat (NLR) disease resistance genes typically confer against races of a single pathogen. Here, we report that Yr87/Lr85, an NLR gene from Aegilops sharonensis and longissima, confers both P. striiformis tritici (Pst) Puccinia triticina (Pt) cause stripe leaf rust, respectively. Yr87/Lr85 Pst Pt in wheat introgression as well transgenic lines. Comparative analysis the cloned Triticeae shows contains two distinct LRR domains is only found Ae. longissima. Allele mining phylogenetic indicate multiple events flow between species presence/absence variation explaining majority to rust species. The confinement longissima highlight potential these valuable sources for improvement. Leaf are important fungal diseases cultivated they caused by infection different pathogens. authors nucleotide-binding protein encoding diseases.
Язык: Английский
Процитировано
11
Nature Plants, Год журнала: 2024, Номер 10(6), С. 971 - 983
Опубликована: Июнь 19, 2024
Язык: Английский
Процитировано
10
Nature Plants, Год журнала: 2024, Номер 10(6), С. 984 - 993
Опубликована: Июнь 19, 2024
Abstract Wheat blast, caused by the fungus Magnaporthe oryzae , threatens global cereal production since its emergence in Brazil 1985 and recently spread to Bangladesh Zambia. Here we demonstrate that AVR-Rmg8 effector, common wheat-infecting isolates, is recognized gene Pm4 previously shown confer resistance specific races of Blumeria graminis f. sp. tritici cause powdery mildew wheat. We show alleles differ their recognition different alleles, some only seedling leaves but not spikes, making it important select for those function both tissues. This study has identified a recognizing an virulence factor present wheat blast isolates Zambia represents first step towards developing durably resistant cultivars these regions.
Язык: Английский
Процитировано
10
Nature, Год журнала: 2024, Номер 636(8043), С. 654 - 662
Опубликована: Ноя. 13, 2024
Pangenomes are collections of annotated genome sequences multiple individuals a species
Язык: Английский
Процитировано
10
PLoS Pathogens, Год журнала: 2023, Номер 19(9), С. e1011294 - e1011294
Опубликована: Сен. 11, 2023
Plant pathogens secrete proteins called effectors that target host cellular processes to promote disease. Recently, structural genomics has identified several families of fungal share a similar three-dimensional structure despite remarkably variable amino-acid sequences and surface properties. To explore the selective forces underlie sequence variability structurally-analogous effectors, we focused on MAX family are major determinants virulence in rice blast fungus Pyricularia oryzae . Using structure-informed gene annotation, 58 78 effector genes per genome set 120 isolates representing seven host-associated lineages. The expression was primarily restricted early biotrophic phase infection strongly influenced by plant. Pangenome analyses demonstrated extensive presence/absence polymorphism loss events possibly involved range adaptation. However, knock-in experiments did not reveal strong effect phenotypes suggesting other evolutionary mechanisms main drivers losses. displayed high levels standing variation rates non-synonymous substitutions, pointing widespread positive selection shaping molecular diversity effectors. combination these with data revealed acts mostly residues located particular elements at specific positions. By providing comprehensive catalog amino acid polymorphism, identifying diversity, our work will inform future studies aimed elucidating function mode action
Язык: Английский
Процитировано
18
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 26, 2024
Abstract Plants and animals respond to pathogen attack by mounting innate immune responses that require intracellular nucleotide binding leucine-rich repeat (NLR) proteins. These receptors detect infection sensing virulence effector However, the mechanisms which evolve new recognition specificities remain poorly understood. Here we report a plant NLR has evolved capacity bind acting as molecular mimic of target effector, thereby triggering an response. The barley Mildew Locus A 3 (MLA3) confers resistance blast fungus Magnaporthe oryzae recognizing Pwl2. Using structural analysis, show MLA3 acquired Pwl2 through mimicry host HIPP43. We demonstrate amino acids at interface are highly conserved in HIPP43 with Pwl2, required trigger used this discovery bioengineer SR50—an MLA ortholog rye wheat stem rust—by introducing MLA3. This chimeric receptor dual activities, responding effectors from two major cereal pathogens. Collectively, these results provide evidence have sophisticated strategies counteract attack.
Язык: Английский
Процитировано
7
PLoS Genetics, Год журнала: 2025, Номер 21(4), С. e1011653 - e1011653
Опубликована: Апрель 9, 2025
Parasites can counteract host immunity by suppressing nucleotide binding and leucine-rich repeat (NLR) proteins that function as immune receptors. We previously showed a cyst nematode virulence effector SPRYSEC15 (SS15) binds inhibits oligomerisation of helper NLR in the expanded NRC1/2/3 clade preventing intramolecular rearrangements required for NRC into an activated resistosome. Here we examined degree to which from multiple Solanaceae species are sensitive suppression SS15 tested hypotheses about adaptive evolution interface between inhibitor proteins. Whereas all orthologs NRC2 were inhibited SS15, some natural variants NRC1 NRC3 insensitive suppression. Ancestral sequence reconstruction combined with functional assays revealed transitioned ancestral suppressed form one over 19 million years ago. Our analyses evolutionary trajectory receptor against parasite inhibitor, identifying key transitions NLRs this inhibition. This work reveals distinct type gene-for-gene interaction or pathogen immunosuppressors receptors contrasts coevolution AVR effectors
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Янв. 23, 2024
Abstract Bioengineering of plant immune receptors has emerged as a key strategy for generating novel disease resistance traits to counteract the expanding threat pathogens global food security. However, current approaches are limited by rapid evolution in field and may lack durability when deployed. Here, we show that rice nucleotide-binding, leucine-rich repeat (NLR) receptor Pik-1 can be engineered respond conserved family effectors from multihost blast fungus pathogen Magnaporthe oryzae . We switched effector binding response profile Pik NLR its cognate AVR-Pik host-determining factor Pwl2 installing putative host target, OsHIPP43, place native integrated HMA domain (generating Pikm-1 OsHIPP43 ). This chimeric also responded other PWL alleles diverse isolates. The crystal structure Pwl2/OsHIPP43 complex revealed multifaceted, robust interface cannot easily disrupted mutagenesis, therefore provide durable, broad isolates carrying field. Our findings highlight how targets used bioengineer new recognition specificities have more properties compared naturally evolved genes.
Язык: Английский
Процитировано
4
Frontiers in Plant Science, Год журнала: 2024, Номер 15
Опубликована: Окт. 9, 2024
Phytopathogens represent an ongoing threat to crop production and a significant impediment global food security. During the infection process, these pathogens spatiotemporally deploy large array of effectors sabotage host defense machinery and/or manipulate cellular pathways, thereby facilitating colonization infection. However, besides their pivotal roles in pathogenesis, certain effectors, known as avirulence (AVR) can be directly or indirectly perceived by plant resistance (R) proteins, leading race-specific resistance. An in-depth understanding intricate AVR-R interactions is instrumental for genetic improvement crops safeguarding them from diseases. Magnaporthe oryzae ( M . ), causative agent rice blast disease, exceptionally virulent devastating fungal pathogen that induces disease on over 50 monocot species, including economically important crops. Rice- M. pathosystem serves prime model functional dissection AVR with R proteins other target due its scientific advantages economic importance. Significant progress has been made elucidating potential interaction between past two decades. This review comprehensively discusses recent advancements field specific focus multifaceted through corresponding R/target during Furthermore, we deliberated emerging strategies engineering leveraging structural insights gained effectors.
Язык: Английский
Процитировано
4