Proteome changes during the germination and early seedling development of carnauba palm (Copernicia prunifera) under skotomorphogenic conditions
Journal of Proteomics,
Год журнала:
2025,
Номер
313, С. 105386 - 105386
Опубликована: Янв. 10, 2025
Язык: Английский
Białka ulegające separacji faz u roślin i metody ich badań
Postępy Biochemii,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 15, 2025
Numerous
scientific
reports
in
recent
years
have
indicated
that
liquid-liquid
phase
separation
(LLPS)
is
an
important
mechanism
allowing
plants
to
identify
various
biotic
and
abiotic
stresses
respond
them.
The
following
paper
reviews
the
methods
currently
used
study
macromolecular
condensates
function
of
proteins
undergoing
LLPS
plants.
Язык: Английский
A PPR Protein RFCD1 Affects Chloroplast Gene Expression and Chloroplast Development in Arabidopsis
Plants,
Год журнала:
2025,
Номер
14(6), С. 921 - 921
Опубликована: Март 15, 2025
Chloroplast
development
is
a
highly
complex
process,
involving
many
regulatory
mechanisms
that
remain
poorly
understood.
This
study
reports
novel
PPR
protein,
RFCD1
(Regulation
Factor
of
Development
1).
Fluorescence
localization
analysis
reveals
the
N-terminal
60
amino
acids
fused
with
GFP
protein
specifically
direct
to
chloroplast.
The
knockout
mutant
embryo-lethal.
RNA
interference
(RNAi)
transgenic
lines
display
chlorosis
phenotypes
and
abnormal
chloroplast
development.
Quantitative
real-time
PCR
(qRT-PCR)
showed
expression
levels
plastid-encoded
polymerase
(PEP)
genes
were
significantly
decreased
in
RNAi
lines.
Furthermore,
blotting
results
RNA-seq
data
processing
plastid
rRNA
was
also
affected
Taken
together,
these
indicate
might
be
involved
gene
processing,
which
essential
for
Arabidopsis.
Язык: Английский
Shedding light on iron nutrition: exploring intersections of transcription factor cascades in light and iron deficiency signaling
Journal of Experimental Botany,
Год журнала:
2024,
Номер
76(3), С. 787 - 802
Опубликована: Авг. 8, 2024
In
the
dynamic
environment
of
plants,
interplay
between
light-dependent
growth
and
iron
nutrition
is
a
recurring
challenge.
Plants
respond
to
low
levels
by
adjusting
physiology
through
enhanced
acquisition
from
rhizosphere
internal
pool
reallocation.
Iron
deficiency
response
assays
gene
co-expression
networks
aid
in
documenting
physiological
reactions
unraveling
gene-regulatory
cascades,
offering
insight
into
hormonal
external
signaling
pathways.
However,
research
directly
exploring
significance
light
remains
limited.
This
review
provides
an
overview
on
regulation
its
cross-connection
with
distinct
signals,
focusing
transcription
factor
cascades
long-distance
signaling.
The
circadian
clock
retrograde
influence
uptake
allocation.
light-activated
shoot-to-root
mobile
ELONGATED
HYPOCOTYL5
(HY5)
affects
homeostasis
responses
roots.
Blue
triggers
formation
biomolecular
condensates
containing
deficiency-induced
protein
complexes.
potential
exploiting
connection
underutilized.
With
climate
change
soil
alkalinity
rise,
there
need
develop
crops
improved
nutrient
use
efficiency
modified
dependencies.
More
needed
understand
leverage
nutrition.
Язык: Английский
Mechanism of nucleus-chloroplast communication by alternative promoter usage and stromules to establish photomorphogenesis in Arabidopsis
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 14, 2024
Abstract
Interorganellar
communication
is
essential
for
maintaining
cellular
and
organellar
functions
adapting
to
dynamic
environmental
changes
in
eukaryotic
cells.
In
plants,
light
triggers
photomorphogenic
development,
including
chloroplast
biogenesis
the
inhibition
of
hypocotyl
elongation,
through
photoreceptors
such
as
red/far-red-sensing
phytochromes
their
downstream
signaling
pathways.
However,
mechanism
interorganellar
crosstalk
underlying
photomorphogenesis
remains
elusive.
Here,
we
investigate
role
light-regulated
alternative
promoter
usage
NUCLEAR
CONTROL
OF
PEP
ACTIVITY
(
NCP
),
a
gene
encoding
phytochrome
component
that
dual-localized
nucleus
chloroplasts.
The
long
transcript
variant
NCP-L
)
upregulated
under
high
red
light,
while
short
NCP-S
predominates
dark
or
low
conditions.
This
transcription
initiation
dependent
on
PHYTOCHROME-INTERACTING
FACTORS
(PIFs).
isoform
primarily
localizes
chloroplasts,
whereas
found
cytoplasm
nucleus.
Notably,
chloroplast-localized
translocates
via
stromules.
Consequently,
NCP-L,
present
both
chloroplasts
nucleus,
initiates
inhibits
growth
during
photomorphogenesis,
nonfunctional
degraded
by
26S
proteasome.
Taken
together,
our
findings
elucidate
mechanisms
which
retrotranslocation
stromules
control
Arabidopsis.
These
provide
insights
into
communication,
orchestrating
organ-specific
developmental
processes
response
fluctuating
environments.
Язык: Английский
PIF transcription factors-versatile plant epigenome landscapers
Frontiers in Epigenetics and Epigenomics,
Год журнала:
2024,
Номер
2
Опубликована: Май 16, 2024
Plants
are
exquisitely
responsive
to
their
local
light
and
temperature
environment
utilizing
these
environmental
cues
modulate
developmental
pathways
adjust
growth
patterns.
This
responsiveness
is
primarily
achieved
by
the
intricate
interplay
between
photoreceptor
phyB
(phytochrome
B)
PIF
(PHYTOCHROME
INTERACTING
FACTORs)
transcription
factors
(TFs),
forming
a
pivotal
signaling
nexus.
PIFs
co-associate
in
photobodies
(PBs)
depending
on
conditions,
can
dissociate
from
PBs
orchestrate
gene
expression.
Until
recently,
mechanisms
governing
epigenome
modifications
subsequent
binding
target
genes
remained
elusive.
mini
review
sheds
emerging
role
of
mediating
reprogramming
recruiting
chromatin
regulators
(CRs).
The
formation
numerous
different
PIF-CR
complexes
enables
precise
temporal
spatial
control
over
regulatory
networks
(GRNs)
plant-environment
interactions.
We
refer
as
landscapers,
while
they
do
not
directly
reprogram
epigenome,
act
critical
sequence-specific
recruitment
platforms
for
CRs.
Intriguingly,
absence
PIFs,
efficacy
largely
compromised
temperature-controlled
processes.
have
thoroughly
examined
composition
function
known
will
explore
also
unanswered
questions
regarding
locations
PIF-mediated
within
genes,
nuclei,
plants.
Язык: Английский