A year at the forefront of plasmodesmal biology DOI Creative Commons
Andrea Paterlini

Biology Open, Год журнала: 2023, Номер 12(10)

Опубликована: Окт. 15, 2023

ABSTRACT Cell–cell communication is a central feature of multicellular organisms, enabling division labour and coordinated responses. Plasmodesmata are membrane-lined pores that provide regulated cytoplasmic continuity between plant cells, facilitating signalling transport across neighboring cells. Plant development survival profoundly depend on the existence functioning these structures, bringing them to spotlight for both fundamental applied research. Despite rich conceptual translational rewards in sight, however, study plasmodesmata poses significant challenges. This Review will mostly focus research published May 2022 2023 intends short overview recent discoveries, innovations, community resources hypotheses.

Язык: Английский

A PDLP-NHL3 complex integrates plasmodesmal immune signaling cascades DOI Creative Commons
Estee E. Tee, Matthew G. Johnston, Diana Papp

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(17)

Опубликована: Апрель 17, 2023

The plant immune system relies on the perception of molecules that signal presence a microbe threat. This triggers transduction mediates range cellular responses via collection molecular machinery including receptors, small molecules, and enzymes. One response to pathogen is restriction cell-to-cell communication by plasmodesmal closure. We previously found while chitin flg22 trigger specialized signaling cascades in plasma membrane, both execute closure callose synthesis at plasmodesmata. Therefore, pathways ultimately converge or upstream synthesis. To establish hierarchy plasmodesmata characterize points convergence elicitor-triggered signaling, we profiled dependence triggered different elicitors machinery. identified that, like chitin, signals RESPIRATORY BURST OXIDASE HOMOLOGUE D (RBOHD) induce Further, PLASMODESMATA-LOCATED PROTEIN 1 (PDLP1), PDLP5, CALLOSE SYNTHASE (CALS1) are common microbe- salicylic acid (SA)-triggered responses, identifying PDLPs as candidate nexus. understand how relay CALS1, screened for PDLP5 interactors NON-RACE SPECIFIC DISEASE RESISTANCE/HIN1 HAIRPIN-INDUCED-LIKE protein 3 (NHL3), which also required chitin-, flg22- SA-triggered PDLP-mediated activation conclude PDLP-NHL3 complex acts an integrating node cascades, transmitting multiple activate CALS1

Язык: Английский

Процитировано

27
Physiological and molecular mechanisms of nitrogen in alleviating drought stress in Phoebe bournei DOI Creative Commons
Jing An, Hongliang Huo, Qiyuan Liu

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 26, 2025

To explore the mechanisms by which nitrogen alleviates drought stress in Phoebe bournei, this study integrated treatment with exogenous application to assess physiological characteristics and employed transcriptome sequencing decipher transcriptional responses. The results indicated that fertilizer mitigated leaf wilting P. bournei under significantly enhanced dry weight, fresh thickness, chlorophyll content. Furthermore, improved photosynthesis inhibiting stomatal closure, enhancing light energy absorption, accelerating electron transport PSII. 11 photosynthesis-related genes, including PFP, TRY, LQY, FTSH, FRO, CURT, PETF, ATPF, PETA, CRRSP, MEN 17 carbohydrate metabolism-associated such as PWD, GBE1, GAPA, PFKA, RFS, ISA, GLGC, PGK, ALDO, GUX, RX9, MIOX, HCT, BAM, MPFP, ERNI exhibited differential expression response nitrogen. Moreover, modulated plant hormone metabolism, 44 upregulated 14 downregulated differentially expressed genes (DEGs) primarily associated jasmonic acid (JA) synthesis signaling. These findings provide new insights into tolerance of context global climate change.

Язык: Английский

Процитировано

0
Role of PMR4 and PDLP1 in priming of early acting penetration defense by resistance-inducing β-amino acids DOI Creative Commons
Chia-Nan Tao, Jurriaan Ton

iScience, Год журнала: 2024, Номер 27(3), С. 109299 - 109299

Опубликована: Фев. 20, 2024

Highlights•The β-amino acids RBH and BABA prime cell wall defenses in Arabidopsis•BABA-primed defense relies on PDLPs PMR4•BABA via different pathways mechanismsSummaryR-β-homoserine (RBH) β-aminobutyric acid (BABA) induce resistance against the oomycete Hyaloperonospora arabidopsidis (Hpa) Arabidopsis, which is based priming of multiple layers, including early acting penetration at wall. Here, we have examined molecular basis RBH- BABA-primed by papillae Hpa. Three-dimensional reconstruction Hpa-induced confocal microscopy revealed no structural differences between control-, RBH-, BABA-treated plants after Hpa challenge. However, mutations affecting POWDERY MILDEW RESISTANCE 4 or PLASMODESMATA LOCATED PROTEINs (PDLPs) only impaired BABA-induced not RBH-induced resistance. Furthermore, PDLP1 over-expression mimicked primed resistance, while intensity GFP-tagged germinating conidiospores was increased but RBH-primed plants. Our study reveals new regulatory layers immune supports notion that a multifaceted layer can be achieved through seperate pathways.Graphical abstract

Язык: Английский

Процитировано

0
Plasmodesmal closure elicits stress responses DOI Creative Commons
Estee E. Tee, Andrew Breakspear, Diana Papp

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 11, 2024

Abstract Plant cells are connected to their neighbors via plasmodesmata facilitating the exchange of nutrients and signaling molecules. During immune responses, close, but how this contributes towards a full response is unknown. To investigate this, we developed two transgenic lines with which could induce plasmodesmal closure independently elicitors, using over-active CALLOSE SYNTHASE3 allele icals3m C-terminus PDLP1 drive callose deposition at plasmodesmata. Induction increased expression stress responsive genes, salicylic acid accumulation resistance Pseudomonas syringae DC3000. More homogeneous also led starch sugars, decreased leaf growth, as well hypersusceptibility Botrytis cinerea . Based on profile conclude that itself activates signaling, raising questions what signals mediate whether these responses occur in all circumstances when close.

Язык: Английский

Процитировано

0
Confocal Microscopy Analysis of Protein Sorting to Plasmodesmata in <i>Nicotiana benthamiana</i> DOI

Yumin Kan,

Eli Simons, Vitaly Citovsky

и другие.

Journal of Visualized Experiments, Год журнала: 2024, Номер 213

Опубликована: Ноя. 1, 2024

Plasmodesmata are membranous nanopores that connect the cytoplasm of adjacent plant cells and enable cell-to-cell trafficking nutrients, macromolecules, as well invading viruses. play fundamental roles in regulation intercellular communication, contributing to development, environmental responses, interactions with viral pathogens. Discovering plasmodesmal localization or proteins could provide useful functional information about protein is important for understanding mechanisms plant-virus interactions. To facilitate these studies, we describe a protocol confocal microscopy-based analysis different targeting select best marker studying virus-plasmodesmata transport. Specifically, analyses events illustrated using movement (MP) Turnip vein-clearing virus (TVCV), Arabidopsis Plasmodesmata-Localized Protein 5 (PDLP5) Callose-Binding 1 (PDCB1). The data analyzed parallel global visualization plasmodesmata aniline blue staining sampled tissues. These approaches can be easily adapted analyze any cellular pathogen planta.

Язык: Английский

Процитировано

0
A year at the forefront of plasmodesmal biology DOI Creative Commons
Andrea Paterlini

Biology Open, Год журнала: 2023, Номер 12(10)

Опубликована: Окт. 15, 2023

ABSTRACT Cell–cell communication is a central feature of multicellular organisms, enabling division labour and coordinated responses. Plasmodesmata are membrane-lined pores that provide regulated cytoplasmic continuity between plant cells, facilitating signalling transport across neighboring cells. Plant development survival profoundly depend on the existence functioning these structures, bringing them to spotlight for both fundamental applied research. Despite rich conceptual translational rewards in sight, however, study plasmodesmata poses significant challenges. This Review will mostly focus research published May 2022 2023 intends short overview recent discoveries, innovations, community resources hypotheses.

Язык: Английский

Процитировано

0